Production of recombinant SERA proteins of Plasmodium falciparum in Escherichia coli by using synthetic genes

Vaccine

Volumn 14, Issue 11, 1996, Pages 1069-1076

  Sugiyama, Tomohiko ^a,d   Suzue, Kazutomo ^a   Okamoto, Munehiro ^b   Inselburg, Joseph ^c   Tai, Kumiko ^a   Horii, Toshihiro ^a  

^a OSAKA UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY MEDICAL SCHOOL   (Japan)

^c DARTMOUTH MEDICAL SCHOOL   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Humoral immune response; Malaria vaccine candidates; SERA protein of Plasmodium falciparum; Synthetic gene

Indexed keywords

MALARIA VACCINE; RECOMBINANT PROTEIN; SYNTHETIC DNA;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; MALARIA; NONHUMAN; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; RAT;

EID: 0030218608     PISSN: 0264410X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0264-410X(95)00238-V     Document Type: Article

References (26)

1. 1 Bzik, D.J., Li, W-b., Horii, T. and Inselburg, J. Amino acid sequence of the serine-repeat antigen (SERA) of Plasmodium falciparum determined from cloned cDNA. Mol. Biochem. Parasitol. 1988, 30, 279-288
2. 2 Banyal, H.S. and Inselburg, J. Isolation and characterization of parasite-inhibitory Plasmodium falciparum monoclonal antibodies. Am. J. Trop. Med. Hyg. 1985, 34, 1055-1064
3. 3 Perrin, L.H., Merkli, B., Loche, M., Chizzolini, C., Smart, J. and Richle, R. Antimalarial Immunity in Saimiri monkeys: immunization with surface components of asexual blood stages. J. Exp. Med. 1984, 160, 441-451
4. 4 Knapp, B., Hundt, E., Nau, U. and Küpper, H.A. Molecular cloning, genomic structure and localization in a blood stage antigen of Plasmodium falciparum characterized by a serine stretch. Mol. Biochem. Parasitol. 1989, 32, 73-84
5. 5 Delplace, P., Fortier, B., Tronchin, G., Dubremetz, J.-F. and Vernes, A. Localization, biosynthesis, processing and isolation of a major 126 kDa antigen of the parasitophrous vacuole of Plasmodium falciparum. Mol. Biochem. Parasitol. 1987, 23, 193-201
6. 6 Chulay, J.D., Lyon, J.A., Haynes, J.D., Meierovics, A.I., Atkinson, C.T. and Aikawa, M. Monoclonal antibody characterization of Plasmodium falciparum antigens in immune complexes formed when schizonts rupture in the presence of immune serum. J. Immun. 1987, 139, 2768-2774
7. 7 Horii, T., Bzik, D.J. and Inselburg, J. Characterization of antigen-expressing Plasmodium falciparum cDNA clones that are reactive with parasite inhibitory antibodies. Mol. Biochem. Parasitol. 1988, 30, 9-18
8. 8 Debrabant, A. and Delplace, P. Leupeptin alters the proteolytic processing of P126, the major parasitophorous vacuole antigen of Plasmodium falciparum. Mol. Biochem. Parasitol. 1989, 33, 151-158
9. 9 Higgins, D.G., McConnell, D.J. and Sharp, P.M. Malarial proteinase? Nature 1989, 340, 604
10. 10 Eakin, A.E., Higaki, J.N., McKerrow, J.H. and Craik, C.S. Cysteine or serine proteinase? Nature 1989, 342, 132
11. 11 Perkins, M.E. and Ziefer, A. Preferential binding of Plasmodium falciparum SERA and rhoptry proteins to erythrocyte membrane inner leaflet phospholipids, Infect. Immun. 1994, 62, 1207-1212
12. 12 Inselburg, J., Bzik, D.J., Li, W.-B. et al. Protective immunity induced in Aotus monkeys by recombinant SERA proteins of Plasmodium falciparum. Infect. Immun. 1991, 59, 1247-1250
13. 13 Inselburg, J., Bathurst, I.C., Kansopon, J., Barchfeld, G.L., Barr, P.J. and Rossan, R.N. Protective immunity induced in Aotus monkeys by a recombinant SERA protein of Plasmodium falciparum: adjuvant effects on induction of protective immunity. Infect. Immun. 1993, 61, 2041-2047
14. 14 Inselburg, J., Bathurst, I.C., Kansopon, J., Barr, P.J. and Rossan, R. Protective immunity induced in Aotus monkeys by a recombinant SERA protein of Plasmodium falciparum: further studies using SERA 1 and MF75.2 adjuvant. Infect. Immun. 1993, 61, 2048-2052
15. 15 Sano, G., Morimatsu, K. and Horii, T. Purification and characterization of dihydrofolate reductase of Plasmodium falciparum expressed by a synthetic gene in Escherichia coli. Mol. Biochem. Parasitol. 1994, 63, 265-273
16. 16 Studier, F.W., Rosenberg, A.M., Dunn, J.J. and Dubendorff, J.W. Use of T7 RNA ploymerase to direct expression of cloned genes. Meth. Enzymol. 1990, 185, 60-89
17. 17 Morimatsu, K. and Horii, T. Analysis of the DNA binding site of Escherichia coli RecA protein. Adv. Biophys. 1995, 31, 23-48
18. 18 Sambrook, J., Fritsch, E.F. and Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory press, Cold Spring Harbor, New York, 1989
19. 19 Sanger, F., Nicklen, S. and Coulson, A.R. DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA 1977, 74, 5463-5467
20. 20 Trager, W. and Jensen, J.B. Human malaria parasites in continuous culture. Science 1976, 193, 673-675
21. 21 Engvall, E. and Perlmann, P. Enzyme-linked immunosorbent assay, ELISA.III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. J. Immunol. 1972, 109, 129-135
22. 22 Towbin, H., Staehelin, T., and Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gel to nitrocellulose sheets: procedure and some applications. Proc. Natl Acad. Sci. USA 1979, 76, 4350-4354
23. 23 Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685
24. 24 Debrabant, A., Maes, P., Delplace, P., Dubremetz, J.-F., Tartar, A. and Camus, D. Intramolecular mapping of Plasmodium falciparum P126 proteolytic fragments by N-terminal amino acid sequencing. Mol. Biochem. Parasitol. 1992 53, 89-96
25. 25 Wada, K., Wada, Y., Ishibashi, F., Gojobori, T. and Ikemura, T. Codon usage tabulated from the GenBank genetic sequence data. Nucl. Acid Res. 1992, 20, 2111-2118
26. 26 Barr, P.J., Inselburg, J., Green, K.M. et al. Immunogenicity of recombinant Plasmodium falciparum SERA proteins in rodents. Mol. Biochem. Parasitol. 1991 45, 159-170