Overproduction and purification of σ(s), the Escherichia coli stationary phase specific sigma transcription factor

Protein Expression and Purification

Volumn 8, Issue 1, 1996, Pages 17-22

  Nguyen, Lam H ^a   Burgess, Richard R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DRUG DERIVATIVE; PRIMER DNA; SARCOSINE; SARKOSYL; SIGMA FACTOR;

ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETIC TRANSCRIPTION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MOLECULAR CLONING; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN CONFORMATION;

CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; PLASMIDS; PROMOTER REGIONS (GENETICS); PROTEIN CONFORMATION; SARCOSINE; SIGMA FACTOR; TRANSCRIPTION, GENETIC;

EID: 0030201338     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0069     Document Type: Article

References (19)

1. 1. Burgess, R. R. (1969) Separation and characterization of the subunits of RNA polymerase. J. Biol. Chem. 244, 2168-2176.
2. 2. Helmann, J. D., and Chamberlin, M. J. (1988) Structure and function of bacterial sigma factors. Annu. Rev. Biochem. 57, 839-872.
3. 3. Lange, R., and Hengge-Aronis, R. (1991) Identification of a central regulator of stationary-phase gene expression in Escherichia coli. Mol. Microbiol. 5, 49-59.
4. 38, is a second principal σ factor of RNA polymerase in stationary phase Escherichia coli. Proc. Natl. Acad. Sci. USA 90, 3511-3515.
5. s, is positively regulated by ppGpp. J. Bacteriol. 175, 7982-7989.
6. 6. Huisman, G., and Kolter, R. (1994) Sensing starvation: Homoserine lactone as signaling molecule in the rpoS response of Escherichia coli. Science 265, 537-539.
7. s-dependent genes in Escherichia coli. J. Bacteriol. 177, 413-422.
8. s subunit of RNA polymerase in Escherichia coli is controlled at the levels of transcription, translation, and protein stability. Genes Dev. 8, 1600-1612.
9. 9. Nguyen, L. H., Jensen, D. B., Thompson, N. E., Gentry, D. R., and Burgess, R. R. (1993) In vitro functional characterization of overproduced Escherichia coli KatF/rpoS gene product. Biochemistry 32, 1112-1117.
10. 10. Lesley, S. A., Brow, M. A. D., and Burgess, R. R. (1991) Use of in vitro protein synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies. J. Biol. Chem. 266, 2632-2638.
11. 11. Mulvey, M. R., and Loewen, P. C. (1989) Nucleotide sequence of katF of Escherichia coli suggests that katF protein is a novel σ transcription factor. Nucleic Acids Res. 17, 9979-9991.
12. 32, the Escherichia coli heat shock transcription factor. Protein Expression Purif. 4, 425-433.
13. 13. Studier, F. W., Rosenberg, A., Dunn, J., and Dubendorff, J. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185, 60-89.
14. 14. Langley, K., Berg, T., Strickland, T., Fenton, D., Boone, T., and Wypych, J. (1987) Recombinant-DNA-derived bovine growth hormone from Escherichia coli. Eur. J. Biochem. 163, 313-321.
15. 15. Gentry, D., and Burgess, R. R. (1990) Overproduction and purification of the ω subunit of Escherichia coli RNA polymerase. Protein Expression Purif. 1, 81-86.
16. 16. Burgess, R. R., and Knuth, M. W. (1996) "Strategies in Protein Purification and Characterization" (Marshak, J., Kadonaga, J., Burgess, R., Knuth, M., Lin, S.-H., and Brennan, W., Eds.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
17. 17. Pace, C. N., Vajdos, F., Fee, L., Grimsley, G., and Gray, T. (1995) How to measure and predict the molar absorption coefficient of a protein. Protein Sci. 4, 2411-2423.
18. s) synthesis in Escherichia coli is subject to postranscriptional regulation. J. Bacteriol. 175, 2150-2153.
19. 19. Jovanovich, S. B., Lesley, S. A., and Burgess, R. R. (1989) In vitro use of monoclonal antibodies in Escherichia coli S-30 extracts to determine the RNA polymerase σ subunit required by a promoter. J. Biol. Chem. 264, 3794-3798.