Influence of bacterial age and pH of lysis buffer on type of nucleic acid isolated

Journal of Microbiological Methods

Volumn 26, Issue 1-2, 1996, Pages 133-138

  Uyttendaele, M ^a   Schukkink, R ^b   Van Gemen, B ^b   Debevere, J ^a  

^a GHENT UNIVERSITY   (Belgium)

^b Organon Teknika   (Belgium)

Author keywords

DNA; Isolation; RNA; Silica

Indexed keywords

BUFFER; DNA; RNA;

ARTICLE; BACTERIUM; CAMPYLOBACTER JEJUNI; CELL AGING; DNA DETERMINATION; DNA PURIFICATION; ESCHERICHIA COLI; LISTERIA MONOCYTOGENES; NONHUMAN; PH; PRIORITY JOURNAL; PSEUDOMONAS FLUORESCENS; RNA ANALYSIS; RNA PURIFICATION;

BACTERIA (MICROORGANISMS); CAMPYLOBACTER JEJUNI; ESCHERICHIA; ESCHERICHIA COLI; LISTERIA MONOCYTOGENES; NEGIBACTERIA; POSIBACTERIA; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 0030201067     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/0167-7012(96)00904-9     Document Type: Article

References (7)

1. [1] Soumet, C., Ermel, G., Fach, P. and Colin, P. (1994) Evaluation of different DNA extraction procedures for the detection of Salmonella from chicken products by polymerase chain reaction. Lett. Appl. Microbiol. 19, 294-298.
2. [2] Wallace, D.M. (1987). Large-and small-scale phenol extractions. Methods Enzymol. 152, 33-41.
3. [3] MacDonald, R.J., Swift, G.H., Przybyla, A.E. and Chirgwin, J.M. (1987) Isolation of RNA using guanidinium salts. Methods Enzymol. 152, 219-227.
4. [4] Boom, R., Sol, C.J.A., Salimans, M.M.M., Jansen, C.L., Wertheim-van Dillen, P.M.E. and van der Noordaa, J. (1990) Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28, 495-503.
5. [5] Masters, C.I., Shallcross, J.A. and Mackey, B.M. (1994) Effect of stress treatments on the detection of Listeria monocytogenes and enterotoxigenic Escherichia coli by the polymerase chain reaction. J. Appl. Bact. 77, 73-79.
6. [6] Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal. Biochem. 162, 156-159.
7. [7] Wolcott, M.J. (1991) DNA-based rapid methods for the detection of foodborne pathogens. J. Food Protect. 54, 387-401.