메뉴 건너뛰기




Volumn 19, Issue 1, 1996, Pages 68-73

Heterologous expression of full-length and truncated forms of the recombinant guluronate-specific alginate lyase of Klebsiella pneumoniae

Author keywords

Alginase; Alginate; Inclusion bodies; Protein expression

Indexed keywords

ALGAE; BACTERIA; CELLS; CLONING; DEGRADATION; DNA; GENES; MUTAGENESIS; POLYSACCHARIDES; PROTEINS;

EID: 0030200080     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/0141-0229(95)00175-1     Document Type: Article
Times cited : (6)

References (25)
  • 2
    • 0002570955 scopus 로고
    • Polysaccharides for food and pharmaceutical uses
    • (Guiry, M. D. and Blunden, G., Eds.). John Wiley & Sons, Chicester
    • Indergaard, M. and Østgaard, K. Polysaccharides for food and pharmaceutical uses. In: Seaweed Resources in Europe: Uses and Potential (Guiry, M. D. and Blunden, G., Eds.). John Wiley & Sons, Chicester, 1991, 169-184
    • (1991) Seaweed Resources in Europe: Uses and Potential , pp. 169-184
    • Indergaard, M.1    Østgaard, K.2
  • 3
    • 0025328709 scopus 로고
    • Cloning of Pseudomonas aeruginosa algG which controls alginate structure
    • Chitnis, C. E. and Ohman, D. E. Cloning of Pseudomonas aeruginosa algG which controls alginate structure. J. Bacteriol. 1990, 172, 2894-2900
    • (1990) J. Bacteriol. , vol.172 , pp. 2894-2900
    • Chitnis, C.E.1    Ohman, D.E.2
  • 4
    • 0001702531 scopus 로고
    • A study of the constituents of alginic acid by partial hydrolysis
    • Haug, A., Larsen, B., and Smidsrød, O. A study of the constituents of alginic acid by partial hydrolysis. Acta Chem. Scand. 1966, 20, 183-190
    • (1966) Acta Chem. Scand. , vol.20 , pp. 183-190
    • Haug, A.1    Larsen, B.2    Smidsrød, O.3
  • 5
    • 0026556379 scopus 로고
    • Enzymic degradation of alginates
    • Gacesa, P. Enzymic degradation of alginates. Int. J. Biochem. 1992, 24, 545-552
    • (1992) Int. J. Biochem. , vol.24 , pp. 545-552
    • Gacesa, P.1
  • 6
    • 0001171690 scopus 로고
    • Isolation conditions for high yields of protoplasts from Laminaria saccharina and L. digitata (Phaeophyceae)
    • Butler, D. M. Østgaard, K., Boyen, C., Evans, L. V., Jensen, A., and Kloareg, B. Isolation conditions for high yields of protoplasts from Laminaria saccharina and L. digitata (Phaeophyceae). J. Exp. Biol. 1989, 40, 1237-1246
    • (1989) J. Exp. Biol. , vol.40 , pp. 1237-1246
    • Butler, D.M.1    Østgaard, K.2    Boyen, C.3    Evans, L.V.4    Jensen, A.5    Kloareg, B.6
  • 7
    • 0027657485 scopus 로고
    • Production and characterization of guluronate lyase from Klebsiella pneumoniae for applications in seaweed biotechnology
    • Østgaard, K., Knutsen, S. H., Dyrset, N., and Aasen, I. M. Production and characterization of guluronate lyase from Klebsiella pneumoniae for applications in seaweed biotechnology. Enzyme Microb. Technol. 1993, 15, 756-763
    • (1993) Enzyme Microb. Technol. , vol.15 , pp. 756-763
    • Østgaard, K.1    Knutsen, S.H.2    Dyrset, N.3    Aasen, I.M.4
  • 8
    • 0028246137 scopus 로고
    • Development of a fermentation process for production of an alginate G-lyase from Klebsiella pneumoniae
    • Dyrset, N., Lystad, K. Q., and Levine, D. W. Development of a fermentation process for production of an alginate G-lyase from Klebsiella pneumoniae. Appl. Microbiol. Biotechnol. 1994, 41, 523-530
    • (1994) Appl. Microbiol. Biotechnol. , vol.41 , pp. 523-530
    • Dyrset, N.1    Lystad, K.Q.2    Levine, D.W.3
  • 9
    • 0026572241 scopus 로고
    • Development of a process for large-scale chromatographic purification of an alginate lyase from Klebsiella pneumoniae
    • Aasen, I. M., Folkvord, K., and Levine, D. W. Development of a process for large-scale chromatographic purification of an alginate lyase from Klebsiella pneumoniae. Appl. Microbiol. Biotechnol. 1992, 37, 55-60
    • (1992) Appl. Microbiol. Biotechnol. , vol.37 , pp. 55-60
    • Aasen, I.M.1    Folkvord, K.2    Levine, D.W.3
  • 10
    • 0024500497 scopus 로고
    • Molecular cloning and heterologous expression of a Klebsiella pneumoniae gene encoding alginate lyase
    • Caswell, R. C., Gacesa, P., Lutrell, K. E., and Weightman, A. J. Molecular cloning and heterologous expression of a Klebsiella pneumoniae gene encoding alginate lyase. Gene 1989, 75, 127-134
    • (1989) Gene , vol.75 , pp. 127-134
    • Caswell, R.C.1    Gacesa, P.2    Lutrell, K.E.3    Weightman, A.J.4
  • 11
    • 0028180198 scopus 로고
    • Alginate lyase of Klebsiella pneumoniae, subsp. aerogenes: Gene cloning, sequence analysis, and high-level expression in Escherichia coli
    • Baron, A. J., Wong, T. Y., Hicks, S. J., Gacesa, P., Willcock, D., and McPherson, M. J. Alginate lyase of Klebsiella pneumoniae, subsp. aerogenes: Gene cloning, sequence analysis, and high-level expression in Escherichia coli Gene 1994, 143, 61-66
    • (1994) Gene , vol.143 , pp. 61-66
    • Baron, A.J.1    Wong, T.Y.2    Hicks, S.J.3    Gacesa, P.4    Willcock, D.5    McPherson, M.J.6
  • 14
    • 0025332826 scopus 로고
    • Plate assay for simultaneous detection of alginate lyases and determination of substrate specificity
    • Gacesa, P. and Wusteman, F. S. Plate assay for simultaneous detection of alginate lyases and determination of substrate specificity. Appl. Environ. Microbiol. 1990, 56, 2265-2267
    • (1990) Appl. Environ. Microbiol. , vol.56 , pp. 2265-2267
    • Gacesa, P.1    Wusteman, F.S.2
  • 15
    • 0017521669 scopus 로고
    • Isolation of a poly-α-L-guluronate lyase from Klebsiella aerogenes
    • Boyd, J. and Turvey, J. R. Isolation of a poly-α-L-guluronate lyase from Klebsiella aerogenes. Carbohydr. Res. 1977, 57, 163-171
    • (1977) Carbohydr. Res. , vol.57 , pp. 163-171
    • Boyd, J.1    Turvey, J.R.2
  • 16
    • 0027572062 scopus 로고
    • End-labelled fluorescent polyguluronate and polymannuronate for the assay of alginate lyases
    • Wusteman, F. S. and Gacesa, P. End-labelled fluorescent polyguluronate and polymannuronate for the assay of alginate lyases. Carbohydr. Res. 1993, 241, 237-244
    • (1993) Carbohydr. Res. , vol.241 , pp. 237-244
    • Wusteman, F.S.1    Gacesa, P.2
  • 17
    • 0141439132 scopus 로고
    • 1H-nmr spectroscopy of alginate: Sequential structure and linkage conformations
    • 1H-nmr spectroscopy of alginate: Sequential structure and linkage conformations. Carbohydr. Res. 1983, 118, 255-260
    • (1983) Carbohydr. Res. , vol.118 , pp. 255-260
    • Grasdalen, H.1
  • 18
    • 0017184389 scopus 로고
    • A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
    • Bradford, M. M. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254
    • (1976) Anal. Biochem. , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 19
    • 0014949207 scopus 로고
    • Cleavage of structural proteins during the assembly of the head of bacteriophage T4
    • Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685
    • (1970) Nature , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 20
    • 0026334930 scopus 로고
    • Substitution of active site His-223 in Pseudomonas aeruginosa elastase and expression of the mutated lasB alleles in Escherichia coli show evidence for autoproteolytic processing of proelastase
    • McIver, K., Kessler, E., and Ohman, D. E. Substitution of active site His-223 in Pseudomonas aeruginosa elastase and expression of the mutated lasB alleles in Escherichia coli show evidence for autoproteolytic processing of proelastase. J. Bacteriol. 1991, 173, 7781-7789
    • (1991) J. Bacteriol. , vol.173 , pp. 7781-7789
    • McIver, K.1    Kessler, E.2    Ohman, D.E.3
  • 21
    • 0020649604 scopus 로고
    • The tac promoter: A functional hybrid derived from the trp and lac promoters
    • deBoer, H. A., Comstock, L. J., and Vasser, M. The tac promoter: A functional hybrid derived from the trp and lac promoters. Proc. Natl. Acad. Sci. 1983, 80, 21-25
    • (1983) Proc. Natl. Acad. Sci. , vol.80 , pp. 21-25
    • DeBoer, H.A.1    Comstock, L.J.2    Vasser, M.3
  • 22
    • 0022719327 scopus 로고
    • pHG165: A pBR322 copy number derivative of pUC8 for cloning and expression
    • Stewart, G. S. A. B., Lubinsky-Mink, S., Jackson, C. G., Cassel, S., and Kuhn, J. pHG165: A pBR322 copy number derivative of pUC8 for cloning and expression. Plasmid 1986, 15, 172-181
    • (1986) Plasmid , vol.15 , pp. 172-181
    • Stewart, G.S.A.B.1    Lubinsky-Mink, S.2    Jackson, C.G.3    Cassel, S.4    Kuhn, J.5
  • 24
    • 0025175246 scopus 로고
    • The heterologous expression and cellular localisation of Klebsiella pneumoniae alginate lyase in Escherichia coli
    • Caswell, R. C. and Gacesa, P. The heterologous expression and cellular localisation of Klebsiella pneumoniae alginate lyase in Escherichia coli. Biochem. Soc. Trans. 1990, 18, 929-930
    • (1990) Biochem. Soc. Trans. , vol.18 , pp. 929-930
    • Caswell, R.C.1    Gacesa, P.2
  • 25
    • 0023046815 scopus 로고
    • A new method for predicting signal sequence cleavage sites
    • von Heijne, G. A new method for predicting signal sequence cleavage sites. Nucleic Acid Res. 1986, 14, 4683-4690
    • (1986) Nucleic Acid Res. , vol.14 , pp. 4683-4690
    • Von Heijne, G.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.