Intact noncovalent dimer of estrogen receptor ligand-binding domain can be detected by electrospray ionization mass spectrometry

Steroids

Volumn 61, Issue 7, 1996, Pages 433-438

  Witkowska, H Ewa ^a   Green, Brian N ^b   Carlquist, Mats ^c   Shackleton, Cedric H L ^a  

^a CHILDREN S HOSPITAL OAKLAND RESEARCH INSTITUTE   (United States)

^b MICROMASS UK LTD   (United Kingdom)

^c Karo Bio   (Sweden)

Author keywords

electrospray ionization mass spectrometry; estrogen receptor; ligand binding domain; noncovalent complex

Indexed keywords

DIMER; ESTROGEN; ESTROGEN RECEPTOR;

ARTICLE; IONIZATION; LIGAND BINDING; MASS SPECTROMETRY; MOLECULAR WEIGHT; PROTEIN CONFORMATION; PROTON TRANSPORT;

EID: 0030200078     PISSN: 0039128X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0039-128X(96)00075-X     Document Type: Article

References (24)

1. 1. Kumar V, Chambon P (1988). The estrogen receptor binds tightly to its responsive element as a ligand-induced homodimer. Cell 55:145-156.
2. 2. Notides AC, Lerner N. Hamilton DE (1981). Positive cooperativety of the estrogen receptor. Proc Natl Acad Sci USA 78:4926-4930.
3. 3. Schwartz JA, Skafar DF (1993). Ligand-mediated modulation of estrogen receptor conformation by estradiol analogs. Biochemistry 32:10109-10115.
4. 4. Ali S, Metzger D, Bornert J-M, Chambon P (1993). Modulation of transcription activation by ligand-dependent phosphorylation of the human oestrogen receptor A/B region. EMBO J 12:1153-1160.
5. 5. Fritsch M, Anderson I, Gorski J (1993). Structural characterization of the trypsinized estrogen receptor. Biochemistry 32:14000-14008.
6. 6. Beekman JM, Allan GF, Tsai SY, Tsai M-J, O'Malley BW (1993). Transcriptional activation by the estrogen receptor requires a conformational change in the ligand binding domain. Mol Endocrinol 7:1266-1274.
7. 7. Martin PM, Berthois Y, Jensen EV (1988). Binding of antiestrogens exposes an occult antigenic determinant in the human estrogen receptor. Proc Natl Acad Sci USA 85:2533-2537.
8. 8. Berthois Y, Pons M, Dussert C, Crastes de Paulet A, Martin P-M (1994). Agonist-antagonist activity of anti-estrogens in the human breast cancer cell line MCF-7: a hypothesis for the interaction with a site distinct from the estrogen binding site. Mol Cell Endocrinol 99:259-268.
9. 9. Pakdel F, Reese JC, Katzenellenbogen BS (1993). Identification of charged residues in an N-terminal portion of the hormone-binding domain of the human estrogen important in transcriptional activity of the receptor. Mol Endocrinol 7:1408-1417.
10. 10. Sabbah M, Gouilleux F, Sola B, Reduilh G, Baulieu E-E (1991). Structural differences between the hormone and antihormone estrogen receptor complexes bound to the hormone response element. Proc Natl Acad Sci USA 88:390-394.
11. 11. Ganem B, Li Y-T, Henion JD (1991). Detection of noncovalent receptor-ligand complexes by mass spectrometry. J Am Chem Soc 113:6294-6296.
12. 12. Ganem B, Li Y-T, Henion JD (1991). Observation of noncovalent enzyme-substrate and enzyme-product complexes by ion-spray mass spectrometry. J Am Chem Soc 113:7818-7819.
13. 13. Baca M, Kent SBH (1992). Direct observation of a ternary complex between the dimeric enzyme HIV-1 protease and a substrate-based inhibitor. J Am Chem Soc 114:3992-3993.
14. 14. Ogorzalek Loo RR, Goodlett DR, Smith RD, Loo JA (1993). Observation of a noncovalent ribonuclease S-protein/S-peptide complex by electrospray ionization mass spectrometry. J Am Chem Soc 115:4391-4392.
15. 15. Tang X-J, Brewer F, Saha S, Chemuhevich I, Ens W, Standing KG (1994). Investigation of protein-protein noncovalent interactions in soybean agglutinin by electrospray ionization time-of flight mass spectrometry. Rapid Commun Mass Spectrom 8:750-754.
16. 16. Light-Wahl KJ, Schwartz BL, Smith RD (1994). Observation of the noncovalent quaternary associations of proteins by electrospray ionization mass spectrometry. J Am Chem Soc 116:5271-5278.
17. 17. Smith VF, Schwartz BL, Randall LL, Smith RD (1996). Electrospray mass spectrometric investigation of the chaperone SecB. Protein Sci 5:488-494.
18. 18. Smith RD, Light-Wahl K (1993). The observation of non-covalent interactions in solution by electrospray ionization mass spectrometry: promise, pitfalls and prognosis. J Biol Mass Spectrom 22:493-501.
19. 19. Smith RD, Loo JA, Edmonds CG, Barinaga CJ, Udseth HR (1990). New developments in biochemical mass spectrometry: electrospray ionization. Anal Chem 62:882-899.
20. 20. McDonnell DP, Nawaz Z, Densmore C, Weigel NL, Pham TA, Clark JH, O'Malley BW (1991). High level expression of biologically active estrogen receptor in Saccharomyces cerevisiae. J Steroid Biochem Mol Biol 39:291-297.
21. 21. Hegy GB, Shackleton HL, Carlquist M, Bonn T, Engström O, Sjohölm P, Witkowska HE (1996). Carboxymethylatoin of the human estrogen receptor ligand binding domain-estradiol complex: HPLC/ESMS peptide mapping showed that cysteine 447 does not react with iodoacetic acid. Steroids 61:367-373.
22. 22. Smith RD, Loo JA, Ogorzalek Loo RR, Busman M, Udseth HR (1991). Principles and practice of electrospray ionization-mass spectrometry for large polypeptides and proteins. Mass Spectrom Rev 10:359-451.
23. 23. Fenn JB (1993). Ion formation from charged droplets: roles of geometry, energy, and time. J Am Soc Mass Spectrom 4:524-535.
24. 24. Chowdhury SK, Katta V, Chait BT (1990). Probing conformational changes in proteins by mass spectrometry. J Am Chem Soc 112: 9012-9013.