Site-directed mutagenesis and analysis of second-site revertants indicates a requirement for C-terminal amino acids of PsaB for stable assembly of the photosystem I reaction center complex in chlamydomonas reinhardtii

Photochemistry and Photobiology

Volumn 64, Issue 1, 1996, Pages 46-52

  Lee, Hyeonmoo ^a   Bingham, Scott E ^a   Webber, Andrew N ^a  

^a ARIZONA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; PHOTOSYSTEM I, PSAB SUBUNIT; PROTOZOAL DNA;

AMINO ACID SEQUENCE; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; CHLAMYDOMONAS REINHARDTII; CONFERENCE PAPER; GENETICS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHOTOCHEMISTRY; PHOTOSYNTHESIS; PHOTOSYSTEM I; PROTEIN FOLDING; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CHLAMYDOMONAS REINHARDTII; CONSERVED SEQUENCE; DNA, PROTOZOAN; MEMBRANE PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOTOCHEMISTRY; PHOTOSYNTHETIC REACTION CENTER COMPLEX PROTEINS; PHOTOSYSTEM I PROTEIN COMPLEX; PROTEIN FOLDING; SEQUENCE HOMOLOGY, AMINO ACID;

ANIMALIA; CHLAMYDOMONAS REINHARDTII;

EID: 0030199280     PISSN: 00318655     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1751-1097.1996.tb02420.x     Document Type: Article

References (29)

1. Golbeck, J. H. (1992) Structure and function of photosystem I. Anna. Rev. Plant Physiol. Plant Mol. Biol. 43, 293-324.
2. Golbeck, J. H. and D. A. Bryant (1991) Photosystem I. Curr. Top. Bioenerg. 16, 83-177.
3. Golbeck, J. H. (1994) Photosystem I in Cyanobacteria. In The molecular Biology of Cyanobacteria (Edited by D. A. Bryant), pp. 319-360. Kluwer, Dordrecht, the Netherlands.
4. Fish, L. E., U. Kiick and L. Bogorad (1985) Analysis of the two partially homologous P700 chlorophyll a proteins of photosystem I: predictions based on the primary sequences and features shared by other chlorophyll proteins. In Molecular Biology of the Photosynthetic Apparatus (Edited by K. E. Steinback, S. Bonitz, C. J. Amtzen and L. Bogorad), pp. 111-120. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
5. Büttner, M., D.-L. Xie, H. Nelson, W. Pinther, G. Hauska and N. Nelson (1992) Photosynthetic reaction center genes in green sulfur bacteria and photosystem I are related. Proc. Natl. Acad. Sei. USA 89, 8135-8139.
6. Liebl, U., M. Mockensturn-Wilson, J. T. Trost, D. C. Brune, R. E. Blankenship and W. F. J. Vermaas (1993) Single core polypeptide in the reaction center of the photosynthetic bacterium Heliobacillus mobilis: structural implications and relations to other photosystems. Proc. Natl. Acad. Sei. USA 90, 7124-7128.
7. Schubert, W. D., O. Klukas, N. Krauss, W. Saenger, P. Fromme and H. T. Witt (1996) Present state of the crystal structure analysis of photosystem system I at 4.5 A resolution. In Photosynthesis: From Light to Biosphere, Vol. II (Edited by P. Mathis), pp. 3-11. Kluwer, Dordrecht, the Netherlands.
8. Vallon, O. and L. Bogorad (1993) Topological study of PSI-A and PSI-B, the two large subunits of the photosystem I reaction center. Eur. J. Biocliem. 214, 907-915.
9. Webber, A. N., S. E. Bingham and H. Lee (1995) Genetic engineering of thylakoid protein complexes by chloroplast transformation in Chlamydomonas reinhardtii. Photosynth. Res. 44, 191-205.
10. Smart, L. B., P. V. Warren, J. H. Golbeck and L. McIntosh (1993) Mutational analysis of the structure and biogenesis of the photosystem I reaction center in the cyanobacterium Synechocystis sp. PCC 6803. Proc. Natl. Acad. Sei. USA 90, 1132-1136.
11. Webber, A. N., P. B. Gibbs, J. B. Ward and S. E. Bingham (1993) Site-directed mutagenesis of the photosystem I reaction center in chloroplasts: the proline-cysteine motif. J. Dial. Client. 268, 12990-12995.
12. Cui, L., S. E. Bingham, M. Kühn, H. Käss, W. Lubitz and A. N. Webber (1995) Specific mutagenesis of conserved histidines in the helix Vdomain of PsaB impairs assembly of the photosystem I reaction center without altering spectroscopic characteristics of P700. Biochemistry 34, 1549-1558.
13. Kiick, U., Y. Choquet, M. Schneider, M. Dron and P. Bennoun (1987) Structural and transcription analysis of two homologous genes for the P700 chlorophyll a-apoproteins in Chlamydomonas reinhardtii: evidence for in vivo irans-splicing. EMBO J. 6, 2185-2195.
14. Bingham, S. E., R. Xu and A. N. Webber (1991) Transformation of chloroplasts with the psaB gene encoding a polypeptide of the photosystem I reaction center. FEES Lett. 292, 137-140.
15. Goldschmidt-Clermont, M. (1991) Transgenic expression of aminoglycoside adenine transferase in the chloroplast: a selectable marker for site-directed transformation of Chlamydomonas. Nucleic Acids Res. 19, 40831089.
16. Bingham, S. E. and A. N. Webber (1994) Maintenance and expression of heterologous genes in the chloroplast of Chlamydomonas reinhardtii. J. Appl. Phycol. 6, 239-245.
17. Sueoka, N. (1960) Mitotic replication of deoxyribonucleic acid in Chlamydomonas reinhardtii. Proc. Natl. Acad. Sei. USA 46, 83-91.
18. Boynton, J. E., N. W. Gillham, E. H. Harris, J. P. Hosier, A. M. Johnson, A. R. Jones, B. L. Randolph-Anderson, D. Robertson, T. M. Klein, K. B. Shark and J. C. Sanford (1988) Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science 240, 1534-1538.
19. Sambrook, J., E. F. Fritsch and T. Maniatis (1989) Molecular Cloning. Cold Spring Harbor Press, Cold Spring Harbor, NY.
20. Chua, N.-H. and P. Bennoun (1975) Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. Proc. Nail. Acad. Sei. USA 72, 2175-2179.
21. Ikeuchi, M. and Y. Inoue (1988) Plant Cell Pliysiol. 29, 1233-1239.
22. Xu, Q. and P. R. Chitnis (1995) Organization of photosystem I polypeptides; identification of PsaB domains that may interact with PsaD. Plant Physiol. 108, 1067-1075.
23. Krabben, L., H. Käß, E. Schlodder, M. Kuhn, W. Lubitz, H. Su, S. Bingham and A. Webber (1996) Site-directed mutations of psaB for the study of cofactor-protein and protein-protein interactions of photosystem I. In Proceedings of the Xth International Photosynthesis Congress (Edited by P. Setif). (In press)
24. Fromme, P., W.-D. Schubert and N. Kraub (1994) Structure of photosystem I: suggestions on the docking sites for plastocyanin, ferredoxin and coordination of P700. Biochim. Biophys. Acta 1187, 99-105.
25. Shinozaki, K., M. Ohme, M. Tanaka, T. Wakasugi, N. Hayashida, T. Matsubayashi, N. Zaita, J. Chunwongse, J. Obokata, K. Yamaguchi-Shinozaki, C. Ohto, K. Torazawa, B. Y. Meng, M. Sugita, H. Deno, T. Kamogashira, K. Yamada, J. F. Takaiwa, A. Kato, N. Tohdoh, H. Shimada and M. Sugiura (1986) The complete nucleotide sequence of tobacco chloroplast genome: its gene organisation and expression. EMBO J. 5, 2043-2049.
26. Lehmbeck, J., O. F. Rasmussem, G. B. Boojans, B. R. Jepsen, B. M. Stummann and K. W. Henningsen (1986) Sequence of two genes in chloroplast DNA coding for the 84 and 82 kDa polypeptides of the photosystem I complex. Plant Mol. Dial. 7, 3-10.
27. Kirsch, W., P. Seyer and R. G. Hermann (1986) Nucleotide sequence of the clustered genes for two P700 chlorophyll a apoproteins of the photosystem I reaction center and the ribosomal protein S14 of the spinach plastid chromosome. Curr. Genet. 10, 843-855.
28. Ohyama, K., H. Fukuzawa, T. Kohchi, H. Shirai, T. Sano, S. Sano, K. Umesono, Y. Shiki, M. Takeuchi, Z. Chang, S. Aota, H. Inokuchi and H. Ozeki (I986) Chloroplast gene organization deduced from the complete nucleotide sequence of liverwort Marchantia polymorpha. Nature 322, 572-574.
29. Cantrell, A. and D. A. Bryant (1987) Molecular cloning and nucleotide sequence of the psaA and psaB genes of the cyanobacterium Syneclwcoccus sp. PCC 7002. Plant Mol. Biol. 9, 453-468.