Human fetal brain cells in aggregate culture: A model system to study regulatory processes of the developing human neuropeptide Y (NPY)-producing neuron

International Journal of Developmental Neuroscience

Volumn 14, Issue 4, 1996, Pages 531-539

  Aguila Mansilla, Nelson ^a   Barnea, Ayalla ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Astrocytes; cAMP pathway; Forskolin; NPY production; Phorbol ester; Protein kinase C pathway

Indexed keywords

FORSKOLIN; NEUROPEPTIDE Y; PHORBOL 13 ACETATE 12 MYRISTATE; PROTEIN KINASE C;

ARTICLE; ASTROCYTE; BRAIN CELL; BRAIN FUNCTION; CELL CULTURE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVATION; FETUS; HEMISPHERE; HUMAN; HUMAN CELL; HUMAN TISSUE; MONOLAYER CULTURE; NERVE CELL; NERVOUS SYSTEM DEVELOPMENT; NEUROTRANSMITTER RELEASE; PRIORITY JOURNAL; THINKING;

EID: 0030198923     PISSN: 07365748     EISSN: None     Source Type: Journal    
DOI: 10.1016/0736-5748(95)00088-7     Document Type: Article

References (34)

1. 1. Adrian T. E., Allen J. M., Bloom S. R., Ghatei M. A., Rossor M. N., Roberts G. W., Crow T. J., Tatemoto K. and Polak J. M. (1983) Neuropeptide Y distribution in human brain. Nature 306, 584-586.
2. 2. Allen Y. S., Adrian T. E., Allen J. M., Tatemoto K., Crow T. J., Bloom S. R. and Polak J. M. (1983) Neuropeptide Y distribution in the rat brain. Science 221, 877-879.
3. 3. Barnea A., Anthony E. and Lu G., Cho G. (1993) Morphological differentiation of neuropeptide Y neurons in aggregate cultures of dissociated fetal cortical cells: a model system for glia-neuron paracrine interactions. Brain Res. 625, 313-322.
4. 4. Barnea A., Cho G. (1993) Basic fibroblast growth factor selectively amplifies the functional state of neurons producing neuropeptide Y but not somatostatin in cultures of fetal brain cells: evidence for cooperative interaction with insulin-like growth factor - I. Endocrinology 133, 1895-1898.
5. 5. Barnea A., Cho G., Hajibeigi A., Aguila M. C. and Magni P. (1991) Dexamethasone induced accumulation of neuropeptide Y by aggregating fetal brain cells in culture: a process dependent on the developmental age of the aggregates. Endocrinology 129, 931-938.
6. 6. Barnea A., Cramer O. and Cho G. (1993) Truncated insulin-like growth factor - I. A potent inducer of neuropeptide Y production by aggregate cultures of fetal brain cells. Endocrine J. 1, 11-17.
7. 7. Barnea A., Hajibeigi A., Cho G. and Magni P. (1991) Regulated production and secretion of immunoreactive neuropeptide Y by aggregating fetal brain cells in culture. Neuroendocrinology 54, 7-13.
8. 8. Barnea A., Hajibeigi A., Trant J. M. and Mason J. I. (1990) Expression of steroid metabolizing enzymes by aggregating fetal brain cells in culture: a model for developmental regulation of the progesterone 5α-reductase pathway. Endocrinology 127, 500-502.
9. 9. Beal M. F., Mazurek M. F., Lorenz L. J., Chattha G. K., Ellison D. W. and Martin J. B. (1986) An examination of neuropeptide Y postmortem stability in an animal model simulating human autopsy conditions. Neurosci. Lett. 64, 69-74.
10. 10. Berrettini W. H., Doran A. R., Kelsoe J., Roy A. and Pickar D. (1987) Cerebrospinal fluid neuropeptide Y in depression and schizophrenia. Neuropsychopharmacology 1, 81-83.
11. 11. Chan Palay V. (1987) Somatostatin immunoreactive neurons in the human hippocampus and cortex shown by immunogold/silver intensification on vibratome sections: coexistence with neuropeptide Y neurons, and effects in Alzheimer-type dementia. J comp. Neurol. 260, 201-223.
12. 12. Chan Palay V., Kohler C., Haesler U., Lang W. and Yasargil G. (1986) Distribution of neurons and axons immunoreactive with antisera against neuropeptide Y in the normal human hippocampus. J. comp. Neurol. 248, 360-375.
13. 13. Chronwall B. M., DiMaggio D. A., Massari V. J., Pickel V. M., Ruggiero D. A. and O'Donohue T. L. (1985) The anatomy of neuropeptide-Y-containing neurons in rat brain. Neuroscience 15, 1159-1181.
14. 14. Dawbarn D., Hunt S. P. and Emson P. C. (1984) Neuropeptide Y: regional distribution chromatographic characterization and immunohistochemical demonstration in post mortem human brain. Brain Res. 296, 168-173.
15. 15. de Quidt M. E. and Emson P. C. (1986) Distribution of neuropeptide Y-like immunoreactivity in the rat central nervous system - II. Immunohistochemical analysis. Neuroscience 18, 545-618.
16. 16. Frederiksen S. O., Ekman R., Gottfries C. G., Widerlöv E. and Jonsson S. (1991) Reduced concentrations of galanin, arginine vasopressin, neuropeptide Y and peptide YY in the temporal cortex but not in the hypothalamus of brains from schizophrenics. Acta Psychiatr. Scand. 83, 273-277.
17. 17. Gjerris A., Widerlov E., Werdelin L. and Ekman R. (1992) Cerebrospinal fluid concentrations of neuropeptide Y in depressed patients and in controls. J. Psychiatr. Neurosci. 17, 23-27.
18. 18. Higuchi H., Yang H. Y. and Sabol S. L. (1988) Rat neuropeptide Y precursor gene expression. mRNA structure, tissue distribution, and regulation by glucocorticoids, cyclic AMP, and phorbol ester. J. biol. Chem. 263, 6288-6295.
19. 19. Labarca C. and Paigen K. (1980) A simple, rapid, and sensitive DNA assay procedure. A. Biochem. 102, 344-352.
20. 20. Lerchen R. A., Yum D.-Y., Krajcik R. and Minth-Worby C. A. Transcriptional vs. post-transcriptional control of neuropeptide Y gene expression. (1989) Endocrinology 136 (in press).
21. 21. Lotstra F., Schiffmann S. N., Vanderhaeghen J. J. (1989) Neuropeptide Y-containing neurons in the human infant hippocampus. Brain Res. 478, 211-226.
22. 22. Magni P., Barnea A. (1992) Forskolin and phorbol ester stimulation of neuropeptide Y (NPY) production and secretion by aggregating fetal brain cells in culture: evidence for regulation of NPY biosynthesis at transcriptional and post-transcriptional levels. Endocrinology 130, 976-984.
23. 23. Magni P. and Barnea A. (1993) An early and transient period of protein synthesis is required for induction of neuropeptide Y-mRNA by phorbol ester and forskolin in aggregate cultures of fetal brain cells. Mol. Cell. Endocrinol. 94, 205-211.
24. 24. Masood F., Wadhwa S. and Bijlani V. (1993) An immunohistochemical study of neurotransmitter profiles in developing human visual cortex. Int. J. devl Neurosci. 11, 387-397.
25. 25. Mattson M. P. and Rychlik B. (1990) Cell culture of cryopreserved human fetal cerebral cortical and hippocampal neurons: neuronal development and responses to trophic factors. Brain Res. 522, 204-214.
26. 26. Mattson M. P. and Rychlik B. (1991) Comparison of rates of neuronal development and survival in human and rat cerebral cortical cell cultures. Mech. Ageing Dev. 60, 171-187.
27. 27. McCarthy M., Resnik L., Taub F., Stewart R. V. and Dix R. D. (1991) Infection of human neural cell aggregate cultures with a clinical isolate of cytomegalovirus. J. Neuropathol. exp. Neurol. 50, 441-450.
28. 28. Mikkelsen J. D., Larsen P. J., Kruse-Larsen C., O'Hare M. M. and Schwartz T. W. (1993) Immunohistochemical and chromatographic identification of peptides derived from proneuropeptide Y in the human frontal cortex. Brain Res. Bull. 31, 415-425.
29. 29. Pulliam L., Berens M. E. and Rosenblum M. L. (1988) A normal human brain cell aggregate model for neurobiological studies. J. Neurosci. Res. 21, 521-530.
30. 30. Pulliam L., Herndier B. G., Tang N. M. and McGrath M. S. (1991) Human immunodeficiency virus-infected macrophages produce soluble factors that cause histological and neurochemical alterations in cultured human brains. J. Clin. Invest. 87, 503-512.
31. 31. Sabol S. L. and Higuchi H. (1990) Transcriptional regulation of the neuropeptide Y gene by nerve growth factor: antagonism by glucocorticoids and potentiation by adenosine 3′,5′-monophosphate and phorbol ester. Mol. Endocrinol. 4, 384-392.
32. 32. Suburo A. M., Gibson S. J., Moscoso G., Terenghi G. and Polak J. M. (1992) Transient expression of neuropeptide Y and its C-flanking peptide immunoreactivities in the spinal cord and ganglia of human embryos and fetuses. Neuroscience 46, 571-584.
33. 33. Widdowson P. S., Ordway G. A. and Halaris A. E. (1992) Reduced neuropeptide Y concentrations in suicide brain. J. Neurochem. 59, 73-80.
34. 34. Widerlov E., Lindstrom L. H., Wahlestedt C. and Ekman R. (1988) Neuropeptide Y and peptide YY as possible cerebrospinal fluid markers for major depression and schizophrenia, respectively. J. Psychiatr. Res. 22, 69-79.