Tritrichomonas foetus: Isolation and characterization of the Golgi complex

Experimental Parasitology

Volumn 83, Issue 2, 1996, Pages 174-183

  Morgado Díaz, José Andrés ^a   Monteiro Leal, Luiz Henrique ^a,b   De Souza, Wanderley ^a,b  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE DARCY RIBEIRO   (Brazil)

Author keywords

deep etching; enzymatic assays; galactosyltransferase; Golgi complex; isolation; parasite; Protozoa; subcellular fractionation; tannic acid; Tritrichomonas faetus

Indexed keywords

5' NUCLEOTIDASE; GALACTOSYLTRANSFERASE; GLUTARALDEHYDE; MALATE DEHYDROGENASE (DECARBOXYLATING); REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE; TANNIN;

ARTICLE; CELL FRACTIONATION; CELL ULTRASTRUCTURE; ELECTRON MICROSCOPY; ENZYME ACTIVITY; ENZYME ASSAY; GOLGI COMPLEX; NONHUMAN; PRIORITY JOURNAL; SUCROSE DENSITY GRADIENT CENTRIFUGATION; TRICHOMONAS FOETUS;

PROTOZOA; TRICHOMONAS; TRITRICHOMONAS; TRITRICHOMONAS FOETUS;

EID: 0030198821     PISSN: 00144894     EISSN: None     Source Type: Journal    
DOI: 10.1006/expr.1996.0064     Document Type: Article

References (37)

1. ARONSON, N. N., AND TOUSTER, J. O. 1974. Isolation of rat liver plasma membrane fragments in isotonic sucrose. In "Methods in Enzymology" (S. Fleischer and L. Parcker, Eds.), Vol. 31, pp. 90-102. Academic Press, New York.
2. BENCHIMOL, M., ELIAS, C. A., AND DE SOUZA, W. 1982. Tritrichomonas foetus: Fine structure of freeze-fractured membranes. Journal of Protozoology 29, 348-353.
3. BENCHIMOL, M., KACHAR, B., AND DE SOUZA, W. 1993. The structural organization of the pathogenic protozoan Tritrichomonas foetus as seen in replicas of quick frozen, freeze-fracture and deep etched cells. Biology of the Cell 77, 289-295.
4. BERGERON, J. J. M., EHRENREICH, J. H., SIEKEVITZ, P., AND PALADE, G. E. 1973. Golgi fractions prepared from rat liver homogenates. II. Biochemical characterization. Journal of Cell Biology 59, 73-88.
5. BLOOM, G. S., AND BRASHEAR, T. A. 1989. A novel 58-kDa protein associates with the Golgi apparatus and micro tubules. Journal of Biological Chemistry 264, 16083-16092.
6. CLUETT, E. B., AND BROWN, J. W. 1992. Adhesion of Golgi by proteinaceous interactions: intercisternal bridges as putative adhesive structures. Journal of Cell Science 103, 773-784.
7. DIAMOND, L. S. 1957. The establishment of various trichomonads of animals and man in axenic cultures. Journal of Parasitology 43, 488-490.
8. DUMPHY, W. G., AND ROTHMAN, J. E. 1985. Compartmental organization of the Golgi stack. Cell 42, 13-24.
9. FARQUHAR, M. G. 1985. Progress in unraveling pathways of Golgi traffic. Annual Review of Cell Biology 1,447-488.
10. FARQUHAR, M. G., AND PALADE, G. E. 1981. The Golgi apparatus (complex) - (1954-1981) - From artifact to center stage. Journal of Cell Biology 91, 77s-103s.
11. FISKE, C. H., AND SUBBAROW, Y. 1925. The colorimetric determination of phosphorus. Journal of Biological Chemistry 66, 375-400.
12. GOTTLIEB, M., AND DWYER, D. 1983. Evidence for distinc 5′-and 3′-nucleotidase activities in the surface membrane fraction of Leishmania donovani promastigotes. Molecular and Biochemical Parasitology 7, 303-317.
13. GRAB, D. J., ITO, S., KARA, U. A. K., AND ROVIS, L. 1984. Glycosyltransferase activities in Golgi complex and Endoplasmic reticulum fractions isolated from African trypanosomes. Journal of Cell Biology 99, 569-577.
14. GRIFFITHS, S., AND SIMON, K. 1986. The trans Golgi network: sorting at the exit site of the Golgi complex. Science 234, 438-443.
15. HONIGBERG, B. M., MATTERN, C. F. T., AND DANIEL, W. A. 1971. Fine structure of the mastigont system in Tritrichomonas foetus (Riedmuller). Journal of Protozoology 18, 183-192.
16. HOWELL, K. E., AND PALADE, G. E. 1982. Hepatic Golgi fractions resolved into membrane and content subtractions. Journal of Cell Biology 92, 822-832.
17. HRDÝ, I., MERTENS, E., AND VAN SCHAFTINGEN, E. 1993. Identification, purification and separation of different isozymes of NADP-specific malic enzyme from Tritrichomonas foetus. Molecular and Biochemical Parasitology 57, 253-260.
18. HSU, V. W., YUAN, L. C., NUCHTERN, J. G., LIPPINCOTT-SCHWARTZ, J., HAMMERLING, G. J., AND KLAUSNER, R. D. 1991. A recycling pathway between the endoplasmic reticulum and the Golgi apparatus for retention of unassembled MHC class I molecules. Nature 352, 441-444.
19. LUCOCQ, J. M., BERGER, E. G., AND WARREN, G. 1989. Mitotic Golgi fragments in HeLa cell and their role in the ressembly pathway. Journal of Cell Biology 109, 463-474.
20. MACHAMER, C. E. 1991. Golgi retention signals: Do membranes hold the key? Trends in Cell Biology 1, 141-144.
21. MASTERS, B. S., WILLIANS, CH., JR., AND KAMIN, H. 1967. The preparation and properties of microsomal TPNH-cytochrome c reductase from pig liver. In "Methods in Enzymology (R. W. Estrabrook and M. E. Pullman, Eds.), Vol. 10, pp. 565-573. Academic Press, New York.
22. MARKWELL, M. A. K., HAAS, S. M., BIEBER, L. L., AND TOLBERT, N. E. 1978. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Annual Biochemistry 87, 206-210.
23. MORRÉ, D. J., HAMILTON, R. L., MOLLENHAUER, H. H., MAHLEY, R. W., CUNNINGHAM, W. P., CHEETHAM, R. D., AND LEQUIRE, V. S. 1970. Isolation of a Golgi apparatus-rich fraction from rat liver. I. Method and morphology. Journal of Cell Biology 44, 484-491.
24. MORRÉ, D. J., MERLIN, L. M., AND KEENAN, T. W. 1969. Localization of glycosyltransferase activities in a Golgi apparatus-rich fraction isolated from rat liver. Biochemical and Biophysical Research Communications 37, 813-819.
25. MORRÉ, D. J., AND MOLLENHAUER, H. H. 1964. Isolation of the Golgi apparatus from plant cells. Journal of Cell Biology 23, 295-305.
26. MULLER, M. 1990. Biochemistry of Trichomonas vaginalis. In "Trichomonads Parasitic in Humans" (B. M. Honigberg, Ed.), pp. 54-83. Springer-Verlag, New York.
27. NILSON, T., HOE, M. H., SLUSAREWICZ, P., RABOUILLE, C., WATSON, R., HUNTE, F., WATZELLE, G., BERGER, E. G., AND WARREN, G. 1994. Kin recognition between medial Golgi enzymes in HeLa cells. EMBO Journal 13, 562-574.
28. NILSON, T., PYPAERT, M., HOE, M. H., SLUSAREWICZ, P., BERGER, E. G., AND WARREN, G. 1993. Overlapping distribution of two glycosyltransferase in the Golgi apparatus of HeLa cells. Journal of Cell Biology 120, 5-13.
29. PFEFFER, S. R., AND ROTHMAN, J. F. 1987. Biosynthetic proteins transport and sorting by the endoplasmic reticulum and Golgi complex. Annual Review of Biochemistry 56, 829-852.
30. PRYDE, J. G. 1994. A group of integral membrane proteins of the rat liver Golgi contains a conserved protein of 100 kDa. Journal of Cell Science. 107, 3425-3436.
31. QUEIROZ, R. C. B., SILVA SANTOS, L. M., BENCHIMOL, M., AND DE SOUZA, W. 1991. Cytochemical localization of enzyme markers in Tritrichomonas foetus. Parasitology Research 77, 561-566.
32. ROTH, J. 1987. Subcellular organization of glycosylation in mammalian cells. Biochemical and Biophysica Acta 906, 405-436.
33. ROTH, J., AND BERGER, J. E. 1982. Immunocytochemical localization of galactosyltransferase in HeLa cells: co-distribution with thiaminepyrophosphatase in transGolgi cisternae. Journal of Cell Biology 93, 223-229.
34. SLUSAREWICZ, P., NILSSON, T., HUI, N., WATSON, R., AND WARREN, G. 1994. Isolation of a matrix that binds medial enzymes. Journal of Cell Biology. 124, 405-413.
35. SUBRAMANIAM, V. N., YUSOFF, A. R., WONG, S. H., LIM, G. B., CHEW, L. M., AND HONG, W. 1992. Biochemical fractionation and characterization of protein from Golgi-enriched membranes. Journal of Biochemical Chemistry 267, 12016-12021.
36. SZTUL, E., KAPLIN, A., SAUCAN, L., AND PALADE, G. 1991. Protein traffic between distinct plasma membrane domains: Isolation and characterization of vesicular carriers involved in transcytosis. Cell 64, 81-89.
37. TRINCHERA, M., AND GHIDONI, R. 1989. Two glycosphingolipid sialyltransferases are localized in different sub-Golgi compartments in rat liver. Journal of Biological Chemistry 64, 15766-15769.