Optimizing the targeted chemical nuclease activity of 1,10- phenanthroline-copper by ligand modification

Bioconjugate Chemistry

Volumn 7, Issue 4, 1996, Pages 413-420

  Gallagher, James ^b   Chen, Chi Hong B ^b   Pan, Clark Q ^b   Perrin, David M ^b   Cho, Young Moon ^b   Sigman, David S ^a  

^a Molecular Biology Institute   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL MODIFICATION; COPPER; DNA; EFFICIENCY; ESCHERICHIA COLI; LIGANDS; PROTEINS; REACTION INTERMEDIATES;

CHEMICAL NUCLEASE; CLEAVAGE SITES; DNA SCISSION; DNA-BINDING PROTEIN; NEOCUPROINE; NUCLEASE ACTIVITY; OLIGONUCLEOTIDE BINDING; PHENANTHROLINE DERIVATIVES; PHENANTHROLINES; RING SUBSTITUENTS;

OLIGONUCLEOTIDES;

ESCHERICHIA COLI;

EID: 0030198135     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc960028t     Document Type: Article

References (34)

1. Lerner, R. A., Benkovic, S. J., and Schultz, P. G. (1991) At the Crossroads of Chemistry and Immunology: Catalytic Antibodies. Science 252, 659-667.
2. Thorn, S. N.; Daniels, R. G., Auditor, M.-T. M., and Hilvert, D. (1995) Large rate accelerations in antibody catalysis by strategic use of haptenic charge. Nature 373, 228-230.
3. Szostak, J. W. (1992) In vitro genetics. TIBS 17, 89-93.
4. Malcolm, B. A., Rosenberg, S., Corey, M. J., Allen, J. S., Baetselier, A. d., and Kirsch, J. D. (1989) Site-Directed Mutagenesis of the Catalytic Residues Asp-52 and Glu-35 of Chicken Egg White Lysozyme. Proc. Natl. Acad. Sci. U.S.A. 86, 133-137.
5. 102 in Serine Protease Catalysis. Science 237, 905-909.
6. Wagner, C., and Benkovic, S. (1990) Site directed mutagenesis: a tool for enzyme mechanism dissection. Trends Biotechnol. 8, 263-270.
7. Sigman, D. S., Bruice, T. W., Mazumder, A., and Sutton, C. L. (1993) Targeted Chemical Nucleases. Acc. Chem. Res. 26, 98-104.
8. Pan, C. Q., Landgraf, R., and Sigman, D. S. (1994) DNA Binding Proteins as Site-Specific Nucleases. Mol. Microbiol. 12, 335-342.
9. Wu, J., Perrin, D. M., Sigman, D. S., and Kaback, H. R. (1995) Helix packing of lactose permease in Escherichia coli studied by site-directed chemical cleavage. Proc. Natl. Acad. Sci. U.S.A. 92, 9186-9190.
10. Sigman, D. S., Mazumder, A., and Perrin, D. M. (1993) Chemical Nucleases. Chem. Rev. 93, 2295-2316.
11. Bruice, T. W., Wise, J., Rosser, D. S. E., and Sigman, D. S. (1991) Conversion of Lambda Phage Cro into an Operator-Specific Nuclease. J. Am. Chem. Soc. 113, 5446-5447.
12. Thederahn, T. B., Kuwabara, M. D., Larsen, T. A., and Sigman, D. S. (1989) Nuclease Activity of 1,10-Phenanthroline-copper: Kinetic Mechanism. J. Am. Chem. Soc. 111, 4941-4946.
13. Graham, D. R., Marshall, L. E., Reich, K. A., and Sigman, D. S. (1980) Cleavage of DNA by Coordination Complexes. Superoxide Formation in the Oxidation by 1,10-Phenanthroline-Cuprous Complexes by Oxygen-Relevance to DNA-cleavage Reaction. J. Am. Chem. Soc. 102, 5419-5421.
14. Marshall, L. E.; Graham, D. R., Reich, K. A., and Sigman, D. S. (1981) Cleavage of Deoxyribonucleic Acid by the 1,10-Phenanthroline-Cuprous Complex. Hydrogen Peroxide Requirement and Primary and Secondary Structure Specificity. Biochemistry 20, 244-250.
15. Reich, K. A., Marshall, L. E., Graham, D. R., and Sigman, D. S. (1981) Cleavage of DNA by the 1,10-Phenanthroline-Copper Ion Complex. Superoxide Mediates the Reaction Dependent on NADH and Hydrogen Peroxide. J. Am. Chem. Soc. 103, 3582-3584.
16. 2+ (phen = 1,10-Phenanthroline). A New Candidate for the Catalytic Photoreduction of Carbon Dioxide. Inorg. Chem. 31, 4542-4546.
17. Ellman, G. L., Courtney, K. D., Andres, V., and Feathersome, R. M. (1961) A New and Rapid Colorimetric Determination of Acetylcholinesterase Activity. Biochem. Pharmacol. 7, 89-95.
18. Chen, C.-h. B., and Sigman, D. S. (1986) Nuclease Activity of 1,10-Phenanthroline-copper: Sequence-specific Targeting. Proc. Natl. Acad. Sci. U.S.A. 83, 7147-7151.
19. Pan, C. Q., Feng, J., Finkel, S. E., Landgraf, R., Johnson, R., and Sigman, D. S. (1994) Structure of the Escherichia coli fis protein-DNA complex probed by protein conjugated with 1,10-phenanthroline copper(I) complex. Proc. Natl. Acad. Sci. U.S.A. 91, 1721-1725.
20. Finkel, S. E., and Johnson, R. C. (1992) The Fis protein: it's not just for DNA inversion anymore. Mol. Microbiol. 6, 3257-3265.
21. Yuan, H. S., Finkel, S. E., Feng, J.-A., Kaczor-Grzeskowiak, M., Johnson, R. C., and Dickerson, R. E. (1991) The molecular structure of wild-type and a mutant Fis protein: Relationship between mutational changes and recombinational enhancer function or DNA binding. Proc. Natl. Acad. Sci. U.S.A. 88, 9558-9562.
22. Feng, J.-A., Yuan, H. S., Finkel, S. E., Johnson, R. C., Kaczor-Grzeskowiak, M., and Dickerson, R. E. (1992) The Interaction of Fis Protein with Its DNA-Binding Sequences. Proteins, Vol. 2, pp 1-9, Adenine Press, Guilderland, NY.
23. Sigman, D. S., Graham, D. R., D'Aurora, V., and Stern, A. M. (1979) Oxygen-dependent Cleavage of DNA by the 1,10-Phenanthroline-cuprous Complex. Inhibition of Escherichia coli DNA Polymerase I. J. Biol. Chem. 254, 12269-12272.
24. Spassky, A., and Sigman, D. S. (1985) Nuclease Activity of 1,10-Phenanthroline-Copper Ion. Conformational Analysis and Footprinting of the lac Operon. Biochemistry 24, 8050-8056.
25. Kuwabara, M. D., and Sigman, D. S. (1987) Footprinting DNA-Protein Complexes in Situ following Gel Retardation Assays Using 1,10-Phenanthroline-Copper Ion: Escherichia coli RNA Polymerase-lac Promoter Complexes. Biochemistry 26, 7234-7238.
26. Thederahn, T., Spassky, A., Kuwabara, M. D., and Sigman, D. S. (1990) Chemical Nuclease Activity of 5-Phenyl-1,10-phenanthroline-Copper Ion Detect Intermediates in Transcription Initiation by E. Coli RNA Polymerase. Biochem. Biophys. Res. Commun. 168, 756-762.
27. Mazumder, A., Perrin, D. M., Watson, K. H., and Sigman, D. S. (1993) A transcription inhibitor specific for unwound DNA in RNA polymerase-promoter open complexes. Proc. Natl. Acad. Sci. U.S.A. 90, 8140-8144.
28. Frantz, B., and O'Halloran, T. V. (1990) DNA Distortion Accompanies Transcriptional Activation by the Metal-Responsive Gene-Regulatory Protein MerR. Biochemistry 29, 4747-4751.
29. Buratowski, S., Sopta, M., Greenblatt, J., and Sharp, P. A. (1991) RNA Polymerase II-associated Proteins are Required for a DNA Conformation Change in the Transcription Initiation Complex. Proc. Natl. Acad. Sci. U.S.A. 88, 7509-7513.
30. Perrin, D. M., Mazumder, A., Sadeghi, F., and Sigman, D. S. (1994) Hybridization of a complementary ribooligonucleotide to the transcription start site of the lac UV-5-Escherichia coli RNA polymerase open complex. Potential for gene-specific inactivation reagents. Biochemistry 33, 3848-3854.
31. Sigman, D. S. (1986) Nuclease Activity of 1,10-Phenanthroline-Copper Ion. Acc. Chem. Res. 19, 180-186.
32. Graham, D. R., and Sigman, D. S. (1984) Zinc Ion in E. coli DNA Polymerase: A Reinvestigation. Inorg. Chem. 23, 4188-4191.
33. Goyne, T. E., and Sigman, D. S. (1987) Nuclease Activity of 1,10-Phenanthroline-Copper Ion. Chemistry of Deoxyribose Oxidation. J. Am. Chem. Soc. 109, 2846-2848.
34. Chu, B. C. F., Wahl, G. M., and Orgel, L. E. (1983) Derivatization of Unprotected Polynucleotides. Nucleic Acids Res. 11, 6513-6529.