Expression of pat neuronal nitric oxide synthase in Saccharomyces cerevisiae

Journal of Biotechnology

Volumn 48, Issue 1-2, 1996, Pages 37-41

  Jones, Susan ^a   Tasab, Mohammed ^a   Ogden, Jill E ^a   Ballance, D James ^a   Powell, Michael J ^a  

^a Delta Biotechnology Ltd   (United Kingdom)

Author keywords

Nitric oxide synthase; Saccharomyces cerevisiae; Yeast expression

Indexed keywords

NITRIC OXIDE SYNTHASE; COMPLEMENTARY DNA; ENZYME INHIBITOR; RECOMBINANT PROTEIN;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; EXPRESSION VECTOR; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; RAT; SACCHAROMYCES CEREVISIAE; ANIMAL; BIOSYNTHESIS; BIOTECHNOLOGY; ENZYMOLOGY; GENETICS; METABOLISM; NERVE CELL; NUCLEOTIDE SEQUENCE; PLASMID;

SACCHAROMYCES CEREVISIAE;

ANIMALS; BASE SEQUENCE; BIOTECHNOLOGY; DNA, COMPLEMENTARY; ENZYME INHIBITORS; GENE EXPRESSION; NEURONS; NITRIC OXIDE SYNTHASE; PLASMIDS; PROMOTER REGIONS (GENETICS); RATS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

ANTIBODIES; BIOCONVERSION; DNA; ESTERS; PROTEINS; YEAST;

ARGININE; CITRULLINE; NITRIC OXIDE SYNTHASE; SACCHAROMYCES CEREVISIAE;

ENZYMES;

EID: 0030197785     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-1656(96)01394-6     Document Type: Article

References (16)

1. Beggs, J.D. (1978) Multiple-copy yeast plasmid vectors. Nature 275, 104-109.
2. Bredt, D.S. and Snyder, S.H. (1990) Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme. Proc. Natl. Acad. Sci. USA 87, 682-685.
3. Bredt, D.S., Hwang, P.M., Glatt, C.E., Lowenstein, C., Reed, R.R. and Synder, S.H. (1991) Cloned and expressed nitric oxide synthase structurally resembles cytochrome P450 reductase. Nature 351, 714-718.
4. Charles, I.G., Palmer, R.M., Hickery, M.S., Bayliss, M.T., Chubb, A.P., Hall, V.S., Moss, D.W. and Moncada, S. (1993a) Cloning, characterisation and expression of a cDNA encoding an inducible nitric oxide synthase from the human chondrocyte. Proc. Natl. Acad. Sci. USA 90, 11419-11423.
5. Charles, I.G., Chubb, A., Gill, R., Clare, G., Lowe, P.N., Holmes, L.S., Page, M., Keeling, J.G., Moncada, S. and Riveros-Moreno, V. (1993b) Cloning and expression of a rat neuronal nitric oxide synthase coding sequence in a baculovirus/insect cell system. Biochem. Biophys. Res. Commun. 196, 1481-1489.
6. Charles, I.G., Page, M., Foxwell, N., Knowles, R., Holmes, L.S., Chubb, A., Palmer, R.M.J. and Moncada, S. (1993c) Expression of a human inducible NO synthase cDNA in a baculovirus expression system. Endothelium 1, s10.
7. Cousens, D.J., Wilson, M.J. and Hinchliffe, E. (1990) Construction of a regulated PGK expression vector. Nucl. Acids Res. 18, 1308.
8. Garthwaite, J. (1991) Glutamate nitric oxide and cell-cell signalling in the nervous system. Trends Neurosci. 14, 60-67.
9. Geller, D.A., Lowenstein, C.J., Shapiro, R.A., Nussler, A.K., Di Silvio, M., Wang, S.C., Nakayama, D.K., Simmons, R.L., Snyder, S.H. and Billiar, T.R. (1993) Molecular cloning and expression of inducible nitric oxide synthase from human hepatocytes. Proc. Natl. Acad. Sci. USA 90, 3491-3495.
10. Janssens, S.P., Shimouchi, A., Quertermous, T., Bloch, D.B. and Bloch, K.D. (1992) Cloning and expression of a cDNA encoding a human endothelium derived relaxing factor/nitric oxide synthase. J. Biol. Chem. 267, 14519-14522.
11. Lowenstein, C.J. and Snyder, S.H. (1992) Nitric oxide, a novel biological messenger. Cell 70, 705-707.
12. Lyons, C.R., Orloff, G.J. and Cunningham, J.M. (1992) Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line. J. Biol. Chem. 267, 6370-6374.
13. Marsden, P.A., Schappert, K.T., Chen, H.S., Flowers, M., Sundell, C.L., Wilcox, J.N.. Lamas, S. and Michel, T. (1992) Molecular cloning and characterisation of human endothelial nitric oxide synthase. FEBS Lett. 307, 287-293.
14. Nakane, M., Schmidt, H.H.H.W., Pollock, J.S., Forstermann, U. and Murad, F. (1993) Cloned human brain nitric oxide synthase is highly expressed in skeletal muscle. FEBS Lett. 316, 175-180.
15. Nathan, C. (1992) Nitric oxide as a secretory product of mammalian cells. FASEB J. 6, 3051-3064.
16. Steven, J., Cottingham, I.R., Berry, S.J., Chinery, S.A., Goodey, A.R., Courtney, M. and Ballance, D.J. (1991) Purification and characterisation of plasminogen activator inhibitor 2 produced in Saccharomyces cerevisiae. Eur. J. Biochem. 196, 431-438.