Assessment of the membrane integrity of fresh and stored turkey spermatozoa using a combination of hypo-osmotic stress, fluorescent staining and flow cytometry

Theriogenology

Volumn 46, Issue 1, 1996, Pages 153-163

  Donoghue, A M ^a   Garner, D L ^b   Donoghue, D J ^c   Johnson, L A ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b UNIVERSITY OF NEVADA   (United States)

^c CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

flow cytometry; membrane integrity; osmolarity; poultry; spermatozoa

Indexed keywords

AVES; MELEAGRIS GALLOPAVO;

EID: 0030197533     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0093-691X(96)00150-1     Document Type: Article

References (29)

1. 1. Almlid T, Johnson LA Effect of glycerol level equilibration time and temperature of glycerol addition on post-thaw viability of boar spermatozoa frozen in straws. J Anim Sci 1988;66:2899-2905.
2. 2. Bakst MR, Sexton TJ Fertilizing capacity and ultrastructure of fowl and turkey spermatozoa before and after freezing. J Reprod Fertil 1979;55:1-7.
3. 3. Bakst MR Fertilizing capacity and morphology of fowl and turkey spermatozoa in hypotonie extender. J Reprod Fertil 1980;60:121-127.
4. 4. Bakst MR, Cecil H C, Sexton TJ Modification of the ethidium bromide exclusion procedure for evaluation of turkey sperm. Poultry Sci 1991;70:336-370.
5. 5. Bakst MR, Wishart G, Brillard JP. Oviductal sperm selection, transport, and storage in poultry. Poultry Sci Rev 1994; 5:117-143.
6. 6. Bayyari GR, Cook JR, Harris GC Jr, Macy LB, Slavik MF, Skeeles, JK. The evaluation of chicken spermatozoa using fluorescent staining in a 96-well format. (research note) Poultry Sci. 1990;69:1602-1605.
7. 7. Christensen V L, Bagley LG Efficacy of fertilization in artificially inseminated turkey hens. Poultry Sci. 1989;68:724-729.
8. 8. Donoghue AM, Garner DL, Donoghue DJ, Johnson LA Viability assessment of turkey sperm using fluorescent staining and flow cytometry. Poultry Sci 1995;74:1191-1200.
9. 9. Correa JR, Zavos PM The hypoosmotic swelling test: Its employment as an assay to evaluate the functional integrity of the frozen-thawed bovine sperm membrane. Theriogenology 1994; 42:351-360
10. 10. Ericsson SA, Garner DL, Redelman D, Ahmad K Assessment of the viability and fertilizing potential of cryopreserved bovine spermatozoa using dual fluorescent staining and two flow cytometric systems. Gamete Res 1989;22:355-368.
11. 11. Ericsson SA, Garner DL, Thomas CA, Downing TW, Marshall CE Interrelationships among fluorometric analyses of spermatozoal function, classic semen quality parameters and the fertility of frozen-thawed bovine spermatozoa. Theriogenology 1993;39:1009-1024.
12. 12. Fazano F, Burmeister ML, de Lucio MA, Bottcher Luiz F, Neves PA, Bahamondes L. Correlation between hypoosmotic swelling test and water test to assess human sperm membrane integrity. Andrologia 1993;25:351-353.
13. 13. Fuse H, Ohata S, Sakamoto M, Kazama T, Katayama T Hypoosmotic swelling test with a medium of distilled water. Arch Androl 1993;30:111-116.
14. 14. Garner DL, Pinkel D, Johnson LA, Pace MM Assessment of spermatozoal function using dual fluorescent staining and flow cytometric analysis. Biol Reprod 1986;34:127-138.
15. 15. Garner DL, Johnson LA, Yue ST, Roth B Haugland RP. Dual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide. J Androl 1994;15:620-629.
16. 16. Graham JK, Kunze E, Hammerstedt RH Analysis of sperm cell viability, acrosomal integrity, and mitochondrial function using flow cytometry. Biol Reprod 1990;43:55-64.
17. 17. Haugland RP Handbook of Fluorescent Probes and Research Chemicals. Eugene OR: Molecular Probes, 1989.
18. 18. Jeyendran RS, Van der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJD Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 1984;70:219-228.
19. 19. Lake PE Factors affecting the fertility in poultry, with special reference to artificial insemination. World's Poult Sci J 1983;39:106-117.
20. 20. Lake P E Recent progress in poultry reproduction. World's Poult Sci J 1989;45:53-59.
21. 21. Lomeo AM, Giambersio AM Water-test: a simple method to assess sperm-membrane integrity. Intl J Androl 1991;14:278-282.
22. 22. Luken MR Evaluating optical filter efficiency in a flow cytometer. J Histochem Catacomb 1980;10:1136-1137.
23. 23. SAS Institute. SAS User's Guide Version 5. SAS Institute, Inc. Cary, NC, 1985.
24. 24. Sexton TJ. Comparison of commercial diluents for holding turkey semen 24 hours at 5°C. Poultry Sci 1988;67:131-134.
25. 25. Sexton TJ, Research note: Influence of damaged spermatozoa on the fertility of turkey semen stored for 24 hours at 5°C. Poultry Sci. 1988; 67:1483-1485.
26. 26. Terstappen LW, Michaels RA, Dost R. Loken MR increased light scattering resolution facilitates multidimensional flow cytometric analysis. Cytometery 1990;11:506-512.
27. 27. Thurston RJ. Storage of poultry semen above freezing for 24-48 hours. In: Bakst MR, Wishart GJ (eds) Proceedings of the First International Symposium on Artificial Insemination in Poultry. Poultry Science Association, Savoy, IL, 1995, pp. 107-122.
28. 28. Watson PF, Kunze E, Cramer P, Hammerstedt RH A comparison of critical osmolarity and hydraulic conductivity and its activation energy in fowl and bull spermatozoa. J Androl 1992; 13:131-138.
29. 29. Wishart GJ. Physiological changes in fowl and turkey spermatozoa during in vitro storage. Br Poultry Sci 1989; 30:443-454.