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Volumn 12, Issue 14, 1996, Pages 3374-3376

Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide

Author keywords

[No Author keywords available]

Indexed keywords

CHARACTERIZATION; INTERFACES (MATERIALS); OPTICAL WAVEGUIDES; PROTEINS; SPECTROSCOPIC ANALYSIS; SURFACES;

EID: 0030181284     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la9601162     Document Type: Article
Times cited : (47)

References (45)
  • 1
    • 0000741018 scopus 로고
    • See for example: (a) Dessey, R. E. Anal. Chem. 1989, 61, 1079A-1094A. (b) Stephens, D. A.; Bohn, P. W. Anal. Chem. 1989, 61, 386-390. (c) DeGrandpre, M. D.; Burgess, L. W. Appl. Spectrosc. 1990, 44, 273-279. (d) Sutherland, R. M.; Dähne, C.; Place, J. F.; Ringrose, A. S. Clin. Chem. 1984, 30, 1533-1538.
    • (1989) Anal. Chem. , vol.61
    • Dessey, R.E.1
  • 2
    • 0001126624 scopus 로고
    • See for example: (a) Dessey, R. E. Anal. Chem. 1989, 61, 1079A-1094A. (b) Stephens, D. A.; Bohn, P. W. Anal. Chem. 1989, 61, 386-390. (c) DeGrandpre, M. D.; Burgess, L. W. Appl. Spectrosc. 1990, 44, 273-279. (d) Sutherland, R. M.; Dähne, C.; Place, J. F.; Ringrose, A. S. Clin. Chem. 1984, 30, 1533-1538.
    • (1989) Anal. Chem. , vol.61 , pp. 386-390
    • Stephens, D.A.1    Bohn, P.W.2
  • 3
    • 0025383128 scopus 로고
    • See for example: (a) Dessey, R. E. Anal. Chem. 1989, 61, 1079A-1094A. (b) Stephens, D. A.; Bohn, P. W. Anal. Chem. 1989, 61, 386-390. (c) DeGrandpre, M. D.; Burgess, L. W. Appl. Spectrosc. 1990, 44, 273-279. (d) Sutherland, R. M.; Dähne, C.; Place, J. F.; Ringrose, A. S. Clin. Chem. 1984, 30, 1533-1538.
    • (1990) Appl. Spectrosc. , vol.44 , pp. 273-279
    • DeGrandpre, M.D.1    Burgess, L.W.2
  • 4
    • 0021681339 scopus 로고
    • See for example: (a) Dessey, R. E. Anal. Chem. 1989, 61, 1079A-1094A. (b) Stephens, D. A.; Bohn, P. W. Anal. Chem. 1989, 61, 386-390. (c) DeGrandpre, M. D.; Burgess, L. W. Appl. Spectrosc. 1990, 44, 273-279. (d) Sutherland, R. M.; Dähne, C.; Place, J. F.; Ringrose, A. S. Clin. Chem. 1984, 30, 1533-1538.
    • (1984) Clin. Chem. , vol.30 , pp. 1533-1538
    • Sutherland, R.M.1    Dähne, C.2    Place, J.F.3    Ringrose, A.S.4
  • 5
    • 0016571962 scopus 로고
    • Propagation of totally reflected light in an IOW occurs in discrete modes. The theory that describes this behavior is well-known. See: (a) Marcuse, D. Bell Syst. Tech. J. 1975, 54, 1507-1530. (b) Kogelnik, H. In Topics in Applied Physics; 7 - Integrated Optics; Tamir, T., Ed.; Springer-Verlag: Berlin, 1979; Chapter 2.
    • (1975) Bell Syst. Tech. J. , vol.54 , pp. 1507-1530
    • Marcuse, D.1
  • 6
    • 0016571962 scopus 로고
    • Tamir, T., Ed.; Springer-Verlag: Berlin, Chapter 2
    • Propagation of totally reflected light in an IOW occurs in discrete modes. The theory that describes this behavior is well-known. See: (a) Marcuse, D. Bell Syst. Tech. J. 1975, 54, 1507-1530. (b) Kogelnik, H. In Topics in Applied Physics; 7 - Integrated Optics; Tamir, T., Ed.; Springer-Verlag: Berlin, 1979; Chapter 2.
    • (1979) Topics in Applied Physics; 7 - Integrated Optics
    • Kogelnik, H.1
  • 7
    • 85087191082 scopus 로고    scopus 로고
    • note
    • 2b
  • 14
    • 0003373776 scopus 로고
    • Proteins at Interfaces II: Fundamentals and Applications; Horbett, T. A., Brash, J. L., Eds.; American Chemical Society: Washington, DC
    • (e) Lee, J. E.; Saavedra, S. S. In Proteins at Interfaces II: Fundamentals and Applications; Horbett, T. A., Brash, J. L., Eds.; ACS Symposium Series 602; American Chemical Society: Washington, DC, 1995; pp 269-279.
    • (1995) ACS Symposium Series 602 , pp. 269-279
    • Lee, J.E.1    Saavedra, S.S.2
  • 19
    • 85087191700 scopus 로고    scopus 로고
    • note
    • 8
  • 24
    • 0003373776 scopus 로고
    • Proteins at Interfaces II: Fundamentals and Applications; Horbett, T. A., Brash, J. L., Eds.; American Chemical Society: Washington, DC
    • (e) Brash, J. L.; Horbett, T. A. In Proteins at Interfaces II: Fundamentals and Applications; Horbett, T. A., Brash, J. L., Eds.; ACS Symposium Series 602; American Chemical Society: Washington, DC, 1995; pp 1-23.
    • (1995) ACS Symposium Series 602 , pp. 1-23
    • Brash, J.L.1    Horbett, T.A.2
  • 41
    • 85087189743 scopus 로고    scopus 로고
    • note
    • 2.
  • 42
    • 3743113199 scopus 로고    scopus 로고
    • note
    • Ferricyt c from horse heart (Sigma) was dissolved in sodium phosphate buffer (50 mM, pH 7.2), purified on a Sephadex G-25 gel filtration column, and diluted to a final concentration of 50 μM the same buffer.
  • 43
    • 3743060946 scopus 로고    scopus 로고
    • Manuscript submitted for review
    • Under these conditions, a protein surface coverage of approximately 1 monolayer is obtained. Lee, J. E.; Saavedra, S. S. Manuscript submitted for review.
    • Lee, J.E.1    Saavedra, S.S.2
  • 44
    • 0028404270 scopus 로고
    • We investigated the influence of the imaginary part of the refractive index of the protein film, assuming a surface coverage of 1 monolayer. The absorbances at 550 nm, where the molar absorptivity of ferrocyt c is greatest in the spectral region of interest, were calculated using a rigorous wave model (Li, L. J. Opt. Soc. Am. A 1994, 11, 984-991) and the ray optics model. Good agreement was found (a difference of ca. 10%), which is expected because of the extremely weak absorbance of the monolayer film.
    • (1994) J. Opt. Soc. Am. A , vol.11 , pp. 984-991
    • Li, L.1


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