Insulin produces a biphasic response in tetrahymena thermophila by stimulating cell survival and activating proliferation in two separate concentration intervals

Cell Biology International

Volumn 20, Issue 6, 1996, Pages 437-444

  Christensen, Søren T ^a,b   Quie, Helene ^a   Kemp, Kåre ^a   Rasmussen, Leif ^a  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Cell proliferation; Cell survival; Hemin; Insulin; Insulin fragment B22 B30; Tetrahymena thermophila

Indexed keywords

BOVINE INSULIN; HEMIN; INSULIN;

ARTICLE; CELL CULTURE; CELL DENSITY; CELL PROLIFERATION; CELL SURVIVAL; CONTROLLED STUDY; NONHUMAN; TETRAHYMENA THERMOPHILA;

BOVINAE; TETRAHYMENA; TETRAHYMENA THERMOPHILA;

EID: 0030175270     PISSN: 10656995     EISSN: None     Source Type: Journal    
DOI: 10.1006/cbir.1996.0055     Document Type: Article

References (34)

1. CHRISTENSEN ST, 1993. Insulin rescues the unicellular eukaryote Tetrahymena from dying in a complete, synthetic medium. Cell Biol Int 17: 833-838.
2. G-methyl-L-arginine and methylene blue. Cell Biol Int (in press).
3. CHRISTENSEN ST, RASMUSSEN L, 1992. Evidence for growth factors which control cell multiplication in Tetrahymena thermophila. Acta Protozool 31: 215-219.
4. CHRISTENSEN ST, SCHOUSBOE P, GHILADI M, RASMUSSEN L, 1992. Nutritional stress relieved by addition of hemin and phospholipids. J Comp Physiol B 162: 107-110.
5. CHRISTENSEN ST, SCHOUSBOE P, NIELSEN D, SØNDERGAARD G, RASMUSSEN L, 1993. Cell multiplication in Tetrahymena setosa and Tetrahymena thermophila in synthetic nutrient medium. Effects of ethanol, cholesterol and extracellular medium. Acta Protozool 32: 151-156.
6. CHRISTENSEN ST, WHEATLEY DN, RASMUSSEN MI, RASMUSSEN L, 1995. Mechanisms controlling death, survival and proliferation in a model unicellular eukaryote cell system, Tetrahymena thermophila. Cell Death Diff 2: 301-308.
7. CSABA G, 1994. Phylogeny and ontogeny of chemical signalling: origin and development. Int Rev Cytol 155: 1-48.
8. CSABA G, LANTOS T, 1985. Effect of insulin on the glucose uptake of protozoa. Experienta 31: 1097-1098.
9. DEMEYTZ P, VAN OBBERGHEN E, ROTH J, WOLMER A, BRANDENBURG D, 1978. Mapping of the residues responsible for the negative cooperative of the receptor-binding region of insulin. Nature 273: 504-509.
10. 2. Endo 101: 360-364.
11. GAMMELTOFT S, 1984. Insulin receptors: binding kinetics and structure relationships of insulin. Physiol Rev 64: 1321-1378.
12. HOUSELY MD, WAKELAM MJO, MURPHY GJ, GAWLER DJ, PYNE NJ, 1987. Glucagon stimulates adenylate cyclase through GR2 glucagon receptors: a process which can be attenuated by glucagon stimulating inositol phospholipid metabolism through GR1 glucagon receptors. Biochem Soc Trans 15: 21-24.
13. KIKUCHI K, LARNER J, FREER RJ, DAY AR, 1981a. Supplemental material to effect of insulin fragment on biological activity of insulin and desoctapeptide insulin. I. Potentiation of biological activities. J Biol Chem 256: 9443-9446.
14. KIKUCHI K, LARNER J, FREER RJ, DAY AR, 1981b. Supplemental material to effect of insulin fragment on biological activity of insulin and desoctapeptide insulin. II. Potentiation of biological activities. J Biol Chem 256: 9447-9449.
15. KIKUCHI K, LARNER J, FREER RJ, DAY AR, MORRIS H, DELL A, 1980. Studies on the biological activity of degraded insulins and insulin fragments. J Biol Chem 255: 9281-9288.
16. KÕHIDAI L, BARSONY J, ROTH J, MARX SJ, 1992. Rapid effects of insulin on cyclic GMP location in an intact protozoan. Experientia 40: 476-481.
17. KÕHIDAI L, KARSA J, CSABA G, 1994. Effects of hormones on chemotaxis in Tetrahymena: investigations on receptor memory. Microbios 77: 75-85.
18. KOPPELHUS, U, HELLUNG-LARSEN P, LEICK V, 1994. An improved quantitive assay for chemokinesis in Tetrahymena. Biol Bull 187: 8-15.
19. 2) and GPI of Tetrahymena pyriformis. Biosci Prep (in press).
20. LEICK V, GRAVE M, HELLUNG-LARSEN P, 1995. Signal-peptide-induced sensory behaviour in free ciliates; bioassays and cellular mechanisms. In (Saba B, Müller WEG, Eds). Signalling Mechanisms in Protozoa and Invertebrates. Progr Mol Subcell Biol 17: 61-79.
21. LEROITH D, SHILOACH J, ROTH J, LESNIAK MA, 1980. Evolutionary origins of vertebrate hormones: substances similar to mammalian insulins are native to unicellular organisms. Proc Natl Acad Sci USA 77: 6184-6188.
22. MENUELLE P, PLAS C, TAKETOMI S, 1989. Effects of modified insulin B21-B26 fragments on glycogenesis and on insulin-receptor complex fate in cultured fetal hepatocytes. Mol Cell Endocrinol 66: 143-151.
23. MIRMIRA RG, NAKAGAWA SH, TAGER HS, 1991. Importance of the character and configuration of residues B24, B25 and B26 in insulin-receptor interactions. J Biol Chem 266: 1428-1436.
24. MIRMIRA RG, TAGER HS, 1989. Role of the phenylalanine B24 side chain in directing insulin interactions with its receptor. J Biol Chem 264: 6349-6354.
25. NAGAWA SH, TAGER HS, 1987. Role of the COOH-terminal B-chain domain in insulin-receptor interactions. J Biol Chem 262: 12,054-12,058.
26. NG FM, ZHU SQ, CUI DF, FAN L, HUANG YD, ZHANG YS, 1989. Structure and activity of the B-chain of insulin. Biochem Int 18: 373-381.
27. ORIAS E, BRUNS PJ, 1976. Induction and Isolation of mutants in Tetrahymena. In Prescott DM ed. Methods in Cell Biology. Academic Press. New York, 247-282.
28. SAUNDERS DJ, BRANDENBURG D, SHANG-CHUAN C, CHIH-CHEN W, 1982. Insulin chemistry - pathway to understand insulin action. Endeavour 6: 146-152.
29. SCHOUSBOE P, RASMUSSEN L, 1994. Survival of Tetrahymena thermophila at low initial cell densities; effects of lipids and long-chained alcohols. J Euk Microbiol 41: 195-199.
30. SHILOACH J, RUBINOVITZ C, LEROITH D, 1985. Extractions of insulin-related material and other peptide-hormones from Tetrahymena pyriformis. ACS Symp Ser 271: 175-191.
31. SZABLEWSKI L, ANDRESEN PH, FLORIN-CHRISTENSEN J, FLORIN-CHRISTENSEN M, RASMUSSEN L, 1991. Tetrahymena thermophila: growth in synthetic medium in the presence and absence of glucose. J Protozool 38: 62-65.
32. WEITZEL G, EISELE K, GUGLIELMI H, STOCK W, RENNER R, 1971. Structure and activity of insulin. XI. Biologically active synthetic peptides of the insulin sequence B22-B25. Hoppe-Seyler's Z Physiol Chem 352: 1735-1738.
33. WEITZEL G, EISELE K, STOCK W, 1975. Specificity of the arginine-guanidione group in biologically active tetrapeptides of the insulin sequence B22-B25 (Arg-Gly-Phe-Phe). Hoppe-Seyler's Z Physiol Chem 365: 583-590.
34. WHEATLEY DN, CHRISTENSEN ST, SCHOUSBOE P, RASMUSSEN L, 1993. Signalling in cell growth and death: adequate nutrition alone may not be sufficient for ciliates. Cell Biol Int 17: 817-823.