Analysis of dystrophin in muscular diseases by two-dimensional gel electrophoresis using agarose gels in the first dimension

Acta Neurologica Belgica

Volumn 96, Issue 2, 1996, Pages 102-107

  Hamano, K ^a   Takeya, T ^a   Iwasaki, N ^a   Okoshi, N ^a   Fukubayashi, T ^a   Kirinoki, M ^a   Yao, Y ^a   Hirabayashi, T ^a   Takita, H ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Becker muscular dystrophy; Duchenne muscular dystrophy; Gel electrophoresis; Infectious muscular disease; Two dimensional

Indexed keywords

DYSTROPHIN;

ADOLESCENT; ADULT; ARTICLE; CHEMISTRY; CHILD; DERMATOMYOSITIS; HUMAN; IMMUNOBLOTTING; INFANT; METABOLISM; MIDDLE AGED; MUSCLE; MUSCULAR DYSTROPHY; MYOSITIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRESCHOOL CHILD; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ADOLESCENT; ADULT; CHILD; CHILD, PRESCHOOL; DERMATOMYOSITIS; DYSTROPHIN; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; IMMUNOBLOTTING; INFANT; MIDDLE AGED; MUSCLES; MUSCULAR DYSTROPHIES; MYOSITIS;

EID: 0030161377     PISSN: 03009009     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. ALBARRACIN C. T., GIBORI G. Prolactin action on luteal protein expression in the corpus luteum. Endocrinology, 1991, 129 : 1821-1830.
2. ARAHATA K., HOFFMAN E. P., KUNKEL L. M., ISHIURA S., TSUKAHARA T. et al. Dystrophin abnormalities by immunofluorescence and immunoblot analyses. Proc. Natl Acad. Sci. USA, 1989, 86 : 7154-7158.
3. BULMAN D. E., MURPHY E. G., ZUBRZYCKA-GAARN E. E., WORTON R. G., RAY P. N. Differentiation of Duchenne and Becker muscular dystrophy phenotypes with amino- and carboxy-terminal antisera specific for dystrophin. Am. J. Hum. Genet., 1991, 48 : 295-304.
4. FRANKE W. W., HEID H. W., GRUND C., WINTER S., FREUDENSTEN C. et al. Antibodies to the major insoluble milk fat globule membrane-associated protein : Specific location in apical regions of lactating epithelial cells. J. Cell Biol., 1981, 89 : 485-494.
5. GEE S. H., BLACHER R. W., DOUVILLE P. J., PROVOST P. R., YURCHENCO P. D., CARBONETTO S. Laminin-binding protein 120 from brain 13 closely related to the dystrophin-associated glycoprotein, dystroglycan, and binds with high affinity to the major heparin binding domain of laminin. J. Biol. Chem., 1993, 268 : 14972-14980.
6. HASHIMOTOK., SHIMIZU T., NONAKA I., MANNEN T. Immunochemical analysis of α-actinin of nemaline myopathy after two-dimensional electrophoresis. J. Neurol. Sci., 1989, 93 : 199-209.
7. HIRABAYASHI T. Two-dimensional gel electrophoresis of chicken skeletal muscle proteins with agarose gels in the first dimension. Anal. Biochem., 1981, 117 : 443-451.
8. HORI S., OHTANI S., SHIMIZU T., IBI T., SAHASHI K. et al. Multiplicity of abnormal dystrophin in Becker muscular dystrophy. A Becker muscular dystrophy gene frequently produced two smaller sizes of dystrophin. J. Neurol. Sci., 1994, 121 : 183-189.
9. HORI S., SUGIURA H., SHIMIZU T., HIRABAYASHI T., OHTANI S. et al. Detection of dystrophin on two-dimensional gel electrophoresis. Biochem. Biophys. Res. Commun., 1989, 161 : 726-731.
10. LAEMMLI U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London), 1970, 227 : 680-685.
11. NAKAMURA M., IMAI H., HIRABAYASHI T. Coordinate accumulation of troponin subunits in chiken breast muscle. Dev. Biol., 1989, 132 : 389-397.
12. NICHOLSON L. V. B., DAVISON K., FALKOUS G., HARWOOD C., O'DONNELL E. et al. Dystrophin in skeletal muscle. I. Western blot analysis using a monoclonal antibody. J. Neurol. Sci., 1989, 94 : 125-136.
13. O'FARRELL P. H. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem., 1975, 250 : 4007-4021.
14. SEWRY C. A., CLERK A., HERCKMATT J. Z., VYSE T., DUBOWITZ V. et al. Dystrophin abnormalities in polymyositis and dermato-myositis. Neuromuscul. Disord., 1991, 1 : 333-339.
15. TOWBIN H., STAEHELIN T., GORDON J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : Procedure and some applications. Proc. Natl. Acad. Sci. USA, 1979, 76 : 4350-4354.
16. VOIT T., STUETTGEN P., CREMER M., GOEBEL H. H. Dystrophin as a diagnostic marker in Duchenne and Becker muscular dystrophy. Correlation of immunofluorescence and western blot. Neuropediatrics, 1991, 22 : 152-162.