Characterization of DNA sequences encoding a novel isoform of the 55 kDa B regulatory subunit of the type 2A protein serine/threonine phosphatase of Arabidopsis thaliana

Plant Molecular Biology

Volumn 31, Issue 2, 1996, Pages 419-427

  Corum III, John W ^a   Hartung, Andrew J ^a   Stamey, Robin T ^a   Rundle, Sabine J ^a  

^a WESTERN CAROLINA UNIVERSITY   (United States)

Author keywords

Arabidopsis; PP2A regulatory subunit; serine threonine phosphatase

Indexed keywords

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 0030152650     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00021804     Document Type: Article

References (24)

1. Akiyama N, Shima H, Hatano Y, Osawa Y, Sugimura T, Nagao M: cDNA cloning of BRγ, a novel brain-specific isoform of the B regulatory subunit of type-2A protein phosphatase. Eur J Biochem 230: 766-772 (1995).
2. Ariño J, Pérez-Callejón E, Cunillera N, Camps M, Posas F, Ferrer A: Protein phosphatases in higher plants: multiplicity of type 2A phosphatases in Arabidopsis thaliana. Plant Mol Biol 21: 475-485 (1993).
3. Bosch M, Cayla X, Van Hoof C, Hemmings BA, Ozon R, Merlevede W, Goris J: The PR55 and PR65 subunits of protein phosphatase 2A from Xenopus laevis: molecular cloning and developmental regulation of expression. Eur J Biochem 230: 1037-1045 (1995).
4. Casamayor A, Pérez-Callejón E, Pujol G, Ariño J, Ferrer A: Molecular characterization of a fourth isoform of the catalytic subunit of protein phosphatase 2A from Arabidopsis thaliana. Plant Mol Biol 26: 523-528 (1994).
5. Cohen P: The structure and regulation of protein phosphatases. Annu Rev Biochem 58: 453-508 (1989).
6. Evans IM, Fawcett T, Boulter D, Fordham-Skelton AP: A homologue of the 65 kDa regulatory subunit of protein phosphatase 2A in early pea (Pisum sativum L.) embryos. Plant Mol Biol 24: 689-695 (1994).
7. Hatano Y, Shima H, Haneji T, Miura AB, Sugimura T, Nagao M: Expression of PP2A B regulatory subunit β isotype in rat testis. FEBS Lett 324: 71-75 (1993).
8. Healy AM, Zolnierowicz S, Stapleton AE, Goebl M, DePaoli-Roach AA, Pringle JR: CDC55, a Saccharomyces cerevisae gene involved in cellular morphogenesis: identification, characterization, and homology to the B subunit of mammalian type 2A protein phosphatase. Mol Cell Biol 11: 5767-5780 (1991).
9. Hemmings BA, Adams-Pearson C, Maurer F, Müller P, Goris J, Merlevede W, Hofsteenge J, Stone S: α- and β-forms of the 65-kDa subunit of protein phosphatase 2A have a similar 39 amino acid repeating structure. Biochemistry 29: 3166-3173 (1990).
10. Hendrix P, Turowski P, Mayer-Jaekel RE, Goris J, Hofsteenge J, Merlevede W, Hemmings BA: Analysis of subunit isoforms in protein phosphatase 2A holoenzymes from rabbit and Xenopus. J Biol Chem 268: 7330-7337 (1993).
11. Kieber JJ, Rothenberg M, Roman G, Feldmann KA, Ecker JR: CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the Raf family of protein kinases. Cell 72: 427-441 (1993).
12. Lee TH, Tuck C, Kirschner MW: Inhibition of cdc2 activation by INH/PP2A. Mol Biol Cell 5: 323-338 (1994).
13. Lizardi P, Williamson R, Brown D: The size of fibroin messenger RNA and its polyadenylic acid content. Cell 4: 199-205 (1975).
14. Mayer RE, Hendrix P, Cron P, Matthies R, Stone SR, Goris J, Merlevede W, Hofsteenge J, Hemmings BA: Structure of the 55-kDa regulatory subunit of protein phosphatase 2A: evidence for a neuronal specific isoform. Biochem 30: 3589-3597 (1991).
15. Mayer-Jaekel RE, Baumgartner S, Bilbe G, Ohkura H, Glover DM, Hemmings BA: Molecular cloning and developmental expression of the catalytic and 65-kDa regulatory subunits of protein phosphatase 2A in Drosophila. Mol Biol Cell 3: 287-298 (1992).
16. Mayer-Jaekel RE, Ohkura H, Gomes R, Sunkel CE, Baumgartner C, Hemmings BA, Glover DM: The 55 kD regulatory subunit of Drosophila protein phosphatase 2A is required for anaphase. Cell 73: 621-633 (1993).
17. Mayer-Jaekel RE, Hemmings BA: protein phosphatase 2A: a 'ménage à trois' Trends Cell Biol 4: 287-291 (1994).
18. Pearson W, Lipman D: Improved tools for biological sequence comparison. Proc Natl Acid Sci USA 85: 2444-2448 (1988).
19. Pérez-Callejón E, Casamayor A, Pujol G, Clua E, Ferrer A, Ariño J: Identification and molecular characterization of two homologues of protein phosphatase X from Arabidopsis thaliana. Plant Mol Biol 23: 1177-1185 (1993).
20. Rundle SJ, Hartung AJ, Corum JW, O'Neill M: Characterization of a cDNA encoding the 55 kDa B regulatory subunit of Arabidopsis protein phosphatase 2A. Plant Mol Biol 28: 257-266 (1995).
21. Slabas AR, Fordham-Skelton AP, Fletcher D, Martinez-Rivas JM, Swinhoe R, Croy RRD, Evans IM: Characterization of cDNA and genomic clones encoding homologues of the 65 kDa regulatory subunit of protein phosphatase 2A in Arabidopsis thaliana. Plant Mol Biol 26: 1125-1138 (1994).
22. Smits PHM, Smits HL, Minnaar RP, Hemmings BA, Mayer-Jaekel RE, Schuurman R, van der Noordaa J, ter Schegget J: The 55 kDa regulatory subunit of protein phosphatase 2A plays a role in the activation of the HPV16 long control region in human cells with a deletion in the short arm of chromosome 11. EMBO J 11: 4601-4606 (1992).
23. Stamey RT, Rundle SJ: Characterization of a novel isoform of a type 2A serine/threonine protein phosphatase from Arabidopsis thaliana. Plant Physiol in press (1996).
24. Zolnierowicz S, Csortos C, Bondor J, Verin A, Mumby MC, DePaoli-Roach AA: Diversity in the regulatory B-subunits of protein phosphatase 2A: identification of a novel isoform highly expressed in brain. Biochemistry 33: 11858-11867 (1994).