Partial characterization of a putative 110 kDa myosin from the green alga Chara corallina by in vitro binding of fluorescent F-ACTIN

Cell Biology International

Volumn 20, Issue 5, 1996, Pages 365-373

  Grolig, Franz ^a   Schröder, Jan ^a,b   Sawitzky, Heiko ^a,c   Lange, Ulf ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

^b JOHANNES GUTENBERG UNIVERSITY   (Germany)

^c MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

Actin binding protein; Calmodulin; Chara corallina; F actin; Monoclonal antibody; Myosin; N ethylmaleimide

Indexed keywords

ACTIN BINDING PROTEIN; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; CALCIUM ION; CALMODULIN; F ACTIN; MONOCLONAL ANTIBODY; MYOSIN; N ETHYLMALEIMIDE; POLYPEPTIDE; RHODAMINE;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; GREEN ALGA; IMMUNOBLOTTING; MICROFILAMENT; NONHUMAN; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN LOCALIZATION;

ALGAE; CHARA CORALLINA; CHLOROPHYTA; CORALLINA;

EID: 0030151970     PISSN: 10656995     EISSN: None     Source Type: Journal    
DOI: 10.1006/cbir.1996.0043     Document Type: Article

References (44)

1. ALLAN E, HEPLER PK, 1989. Calmodulin and calcium-binding proteins. Biochem of Plants 15: 455-484.
2. BLUM H, BEIER H, GROSS HJ, 1987. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8: 93-99.
3. BRADFORD MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
4. CHEN JCW, KAMIYA N, 1975. Localization of myosin in the internodal cell of Nitella as suggested by differential treatment with N-ethylmaleimide. Cell Struct Fund 1: 1-9.
5. ESPREAFICIO EM, CHENEY RE, MATTEOLI M, NASCIMENTO AAC, DE CAMILLI PV, LARSON RE, MOOSEKER MS, 1992. Primary structure and cellular localization of chicken brain myosin-V (p190), an unconventional myosin with calmodulin light chains. J Cell Biol 119: 1541-1557.
6. GROLIG F, WAGNER G, 1988. Light-dependent chloroplast reorientation in Mougeotia and Mesotaenium: biased by pigment-regulated plasmalemma anchorage sites to actin filaments? Bot Acta 101: 2-6.
7. 2+-sensitive streaming in the alga Chara: detection of two polypeptides reacting with a monoclonal anti-myosin and their localization in the streaming endoplasm. Eur J Cell Biol 47: 22-31.
8. HARTWIG JH, KWIATHKOWSKI DJ, 1991. Actin-binding proteins. Curr Opin Cell Biol 3: 87-97.
9. HASSON T, MOOSEKER MS, 1995. Molecular motors, membrane movements and physiology: emerging roles for myosins. Curr Biol 7: 587-594.
10. HUG C, JAY YJ, REDDY I, MCNALLY JG, BRIDGMAN PC, ELSON EI, 1995. Capping protein levels influence actin assembly and cell motility in Dictyostelium. Cell 81: 591-600.
11. HYNES TR, BLOCK SM, WHITE BT, SPUDICH JA, 1987. Movement of myosin fragments in vitro: domains involved in force production. Cell 48: 953-963.
12. 2+. Curr Topics Plant Physiol 4: 79-85.
13. KACHAR B, 1985. Direct visualization of organelle movement along actin filaments dissociated from characean algae. Science 227: 1355-1357.
14. KAMIYA N, 1981. Physical and chemical basis of cytoplasmic streaming. Ann Rev Plant Physiol 32: 205-236.
15. KERSEY YM, HEPLER PK, PALEVITZ BA, WESSELS NK, 1976. Polarity of actin filaments in characean algae. Proc Natl Acad Sci USA 73: 165-167.
16. KOHNO T, ISHIKAWA R, NAGATA T, KOHAMA K, SHIMMEN T, 1992. Partial purification of myosin from lily pollen tubes by monitoring with in vitro motility assay. Protoplasma 170: 77-85.
17. KRÖN SM, TOYOSHIMA YY, UYEDA TQP, SPUDICH JA, 1991. Assays for actin sliding movement over myosin-coated surfaces. Meth Enzymol 196: 399-416.
18. LAEMMLI UK, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
19. LANGE U, 1992. Untersuchungen zu Aktin-bindenden Proteinen bei Phaseolus Coccineus und Zea mays, und Sequenzierung eines Gens für ein Aktin-bindendes Protein aus Saccharomyces exiguus. PhD Thesis, Justus-Liebig-University, Giessen, Germany.
20. MCCURDY DW, HARMON AC, 1992. Calcium-dependent protein kinase in the green alga Chara. Planta 188: 54-61.
21. MEEUSEN RE, CANDE WZ, 1979. N-ethylmaleimide-modified heavy meromyosin: a probe for actomyosin interactions. J Cell Biol 82: 57-65.
22. MILLER KG, ALBERTS BM, 1989. F-actin affinity chromatography: Technique for isolating previously unidentified actin-binding proteins. Proc Natl Acad Sci USA 86: 4808-4812.
23. MINEYUKI Y, KATAOKA H, MASUDA Y, NAGAI R, 1995. Dynamic changes in the actin cytoskeleton during the high-fluence rate response of the Mougeotia chloroplast. Protoplasma 185: 222-229.
24. NAGAI R, 1993. Regulation of intracellular movements in plant cells by environmental stimuli. Int Rev Cytol 145: 251-309.
25. PARDEE JD, SPUDICH JA, 1982. Purification of muscle actin. Meth Enzymol 85: 164-182.
26. PARKE J, MILLER C, ANDERTON BH, 1986. Higher plant myosin heavy-chain identified using a monoclonal antibody. Eur J Cell Biol 41: 9-13.
27. POLLARD TD, COOPER JA, 1986. Actin and actin-binding proteins. A critical evaluation of mechanisms and functions. Annu Rev Biochem 55: 987-1035.
28. REEDY MK, 1993. Myosin-actin motors: the partnership goes atomic. Structure 1: 1-5.
29. RUHLANDT G, LANGE U, GROLIG F, 1994. Profilins purified from higher plants bind to actin from cardiac muscle and to actin from a green alga. Plant Cell Physiol 35: 849-854.
30. RUSS U, GROLIG F, WAGNER G, 1991. Changes of cytoplasmic free calcium in the green alga Mougeotia scalaris: monitored with indo-1, and effect on the velocity of the chloroplast movement. Planta 184: 105-112.
31. SAWITZKY H, GROLIG F, 1995. The phragmoplast of the green alga Spirogyra is functionally distinct from the higher plant phragmoplast. J Cell Biol 130: 1359-1371.
32. SCHMIT A-C, LAMBERT, A-M, 1990. Microinjected fluorescent phalloidin in vivo reveals the F-actin dynamics and assembly in higher plant mitotic cells. Plant Cell 2: 129-138.
33. SHIBAOKA H, NAGAI R, 1994. The plant cytoskeleton. Curr Opin Cell Biol 6: 10-15.
34. SHIMMEN T, 1992. The characean cytoskeleton: Dissecting the streaming mechanism. In Menzel D, ed. The Cytoskeleton of the Algae. CRC Press, Boca Raton, 297-314.
35. 2+-controlled cytoplasmic streaming of characean cells. Protoplasma 136: 161-169.
36. TRAAS JA, DOONAN JH, RAWLINS DJ, SHAW PJ, WATTS J, LLOYD CW, 1987. An actin network is present in the cytoplasm throughout the cell cycle of carrot cells and associates with the dividing nucleus. J Cell Biol 105: 387-395.
37. VUGREK O, MENZEL D, 1994. Primary structure and gene organization of an unconventional mini-myosin heavy chain from the green alga Acetabularia. Cell Biol Int 18: 445.
38. 2+, and organization. In: MELKONIAN M, ed. Algal Cell Motility. Chapman & Hall, London, 73-98.
39. WILLIAMSON RE, 1993. Organelle movements. Annu Rev Plant Physiol Plant Mol Biol 44: 181-202.
40. 2+ and cytoplasmic streaming in the alga Chara. Nature 296: 647-651.
41. WOLENSKI JS, 1995. Regulation of calmodulin-binding myosins. Trends Cell Biol 5: 310-316.
42. YAKOTA E, MCDONALD AR, LIU B, SHIMMEN T, PALEVITZ BA, 1995. Localization of a 170 kDa myosin heavy chain in plant cells. Protoplasma 185: 178-187.
43. YAKOTA E, SHIMMEN T, 1994. Isolation and characterization of plant myosin from pollen tubes of lily. Protoplasma 177: 153-162.
44. YAMAMOTO K, KIKUYAMA M, SUTOH-YAMAMOTO N, KAMITSUBO L, 1994. Purification of actin based motor protein from Chara corallina. Proc Japan Acad 70: 175-180.