Diagnosis of human cytomegalovirus infections in the immunocompromised host

Clinical and Diagnostic Virology

Volumn 5, Issue 2-3, 1996, Pages 181-186

  Gerna, Giuseppe ^a   Zavattoni, Maurizio ^a   Percivalle, Elena ^a   Zella, Davide ^a   Torsellini, Maria ^a   Revello, M Grazia ^a  

^a UNIVERSITY OF PAVIA   (Italy)

Author keywords

Competitive PCR; Genome equivalents; HCMV; HCMV antigenemia; HCMV viremia; HCMV infected circulating endothelial cells; Non competitive PCR; Quantitative PCR

Indexed keywords

VIRUS DNA;

ACQUIRED IMMUNE DEFICIENCY SYNDROME; ANTIGEN DETECTION; CYTOMEGALOVIRUS INFECTION; DNA DETERMINATION; HUMAN; HUMAN CYTOMEGALOVIRUS; ORGAN TRANSPLANTATION; PRIORITY JOURNAL; REVIEW; VIREMIA; VIRUS DIAGNOSIS;

CYTOMEGALOVIRUS; HUMAN HERPESVIRUS 5;

EID: 0030150686     PISSN: 09280197     EISSN: None     Source Type: Journal    
DOI: 10.1016/0928-0197(96)00219-X     Document Type: Review

References (25)

1. Fenner, T.E., Garweg, J., Hufer, F.T., Boehnke, M. and Schmitz, H. (1991) Diagnosis of human cytomegalovirus-induced retinitis in human immunodeficiency virus type-1 infected subjects by using the polymerase chain reaction. J. Clin. Microbiol. 29, 2621-2622.
2. Gerna, G., Baldanti, F., Sarasini, A., Furione, M., Percivalle, E., Revello, M.G. et al. (1994a) Effect of foscarnet induction treatment on quantitation of human cytomegalovirus (HCMV) DNA in peripheral blood polymorphonuclear leukocytes and aqueous humor of AIDS patients with HCMV retinitis. Antimicrob. Agents Chemother. 38, 38-44.
3. Gerna, G., Baldanti, F., Zavattoni, M., Sarasini, A., Percivalle, E. and Revello, M.G. (1992a) Monitoring of ganciclovir sensitivity of multiple human cytomegalovirus strains coinfecting blood of an AIDS patient by an immediate-early antigen plaque assay. Antivirus Res. 19, 333-345.
4. Gerna, G., Furione, M., Baldanti, F. and Sarasini, A. (1994b) Comparative quantitation of human cytomegalovirus DNA in blood leukocytes and plasma of transplant and AIDS patients. J. Clin. Microbiol. 32, 2709-2717.
5. Gerna, G., Revello, M.G., Percivalle, E., Zavattoni, M., Parea, M. and Battaglia, M. (1990) Quantification of human cytomegalovirus viremia by using monoclonal antibodies to different viral proteins. J. Clin. Microbiol. 28, 2681-2688.
6. Gerna, G., Zipeto, D., Parea, M., Revello, M.G., Silini, E., Percivalle, E. et al. (1991a) Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia and DNAemia. J. Infect. Dis. 164, 488-498.
7. Gerna, G., Zipeto, D., Parea, M., Revello, M.G., Silini, E., Percivalle, E. et al. (1991b) Monitoring of human cytomegalovirus (HCMV) infections and DHPG treatment in heart transplant recipients (HTRs) by the polymerase chain reaction (PCR). In: M.P. Landini (Ed.), Progress in Cytomegalovirus Research, Elsevier, Amsterdam, pp. 19-22.
8. Gerna, G., Revello, M.G., Percivalle, E. and Morini, F. (1992b) Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia. J. Clin. Microbiol. 30, 1232-1237.
9. Gerna, G., Furione, M., Baldanti, F., Percivalle, E., Comoli, P. and Locatelli, F. (1995) Quantitation of human cytomegalovirus DNA in bone marrow transplant recipients. Brit. J. Hematol. 91, 674-683.
10. Gleaves, C.A., Smith, T.F., Shuster, E.A. and Pearson, G.R. (1984) Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen. J. Clin. Microbiol. 19, 917-919.
11. Gozlan, J., Salord, J.M., Chovaid, C., Duvivier, C., Picard, O., Meyohas, M.C. et al. (1993) Human cytomegalovirus (HCMV) late-mRNA detection in peripheral blood of AIDS patients: diagnostic value for HCMV disease compared with those of viral culture and HCMV DNA detection. J. Clin. Microbiol. 31, 1943-1945.
12. Grefte, A., Blom, N., Van der Giessen, M., Van Son, W. and The, T.H. (1993) Ultrastructural analysis of circulating cytomegalic cells in patients with active cytomegalovirus infection: evidence for virus production and endothelial origin. J. Infect. Dis. 168, 1110-1118.
13. Grefte, J.M.M., van der Giessen, M., van der Gun, B.T.F., van Son, W.J. and The. T.H. (1991) The predominant viral antigen in peripheral blood leukocytes during an active cytomegalovirus (CMV) infection is the lower matrix protein pp65. In: M.P. Landini (Ed.), Progress in Cytomegalovirus Research, Elsevier, Amsterdam, pp. 233-236.
14. Grefte, J.M.M., van der Gun, B.T.F., Schmolke, S., van der Giessen, M., van Son, W.J., Plachter, B. et al. (1992) The lower matrix protein pp65 is the principal viral antigen present in peripheral blood leukocytes during an active cytomegalovirus infection. J. Gen. Virol. 73, 2923-2932.
15. Grossi, P., Minoli, L., Percivalle, E., Irish, W., Vigano', M. and Gerna, G. (1995) Clinical and virological monitoring of human cytomegalovirus infection in 294 heart transplant recipients. Transplantation 59, 847-851.
16. Koffron, A.J., Mueller, K.H., Kaufman, D.B., Stuart, F.P., Patterson, B. and Abecassis, M.I. (1995) Direct evidence using in situ PCR that the endothelial cell and T-lymphocyte harbor latent MCMV. In: Proc. from The Fifth International Conference 'A Multidisciplinary Approach Towards Controlling Cytomegalovirus Disease'. Scand. J. Infect. Dis 99(suppl.), 61 -62.
17. Locatelli, F., Percivalle, E., Comoli, P., Maccario, R., Zecca, M., Giorgiani, G. et al. (1994) Human cytomegalovirus infection in pediatric patients given allogeneic bone marrow transplantation: role of early antiviral treatment for HCMV antigenemia on patients' outcome. Brit. J. Hematol. 88, 64-71.
18. Percivalle, E., Revello, M.G., Vago, L., Morini, F. and Gerna, G. (1993) Circulating endothelial giant cells permissive for human cytomegalovirus (HCMV) are detected in disseminated HCMV infection with organ involvement. J. Clin. Invest. 92, 663-670.
19. Revello, M.G., Percivalle, E., Di Matteo, A., Morini, F. and Gerna, G. (1992) Nuclear expression of the lower matrix protein of human cytomegalovirus in peripheral blood leukocytes of immunocompromised viraemic patients. J. Gen. Virol. 73, 437-442.
20. Revello, M.G., Percivalle, E., Sarasini, A., Baldanti, F., Furione, M. and Gerna, G. (1994) Diagnosis of human cytomegalovirus infection of the nervous system by pp65 detection in polymorphonuclear leukocytes of cerebrospinal fluid from AIDS patients. J. Infect. Dis. 170, 1275-1279.
21. Revello, M.G., Percivalle, E., Zavattoni, M., Parea, M., Grossi, P. and Gerna, G. (1989) Detection of human cytomegalovirus immediate early antigen in leukocytes as a marker of viremia in immunocompromised patients. J. Med. Virol. 29, 88-93.
22. Saltzman, R.L., Quirk, M.R. and Jordan M.C. (1992) High levels of circulating cytomegalovirus DNA reflect visceral organ disease in viremic immunosuppressed patients other than marrow recipients. J. Clin. Invest. 90, 1832-1838.
23. Van der Bij, W., Torensma, R., Van Son, W.J., Anema, J., Schirm, J., Tegzess, A.M. et al. (1988) Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leukocytes. J. Med. Virol. 25, 179-188.
24. Wolf, D.G. and Spector, S.A. (1993) Early diagnosis of human cytomegalovirus disease in transplant recipients by DNA amplification in plasma. Transplantation 56, 330-334.
25. Zipeto, D., Baldanti, F., Zella, D., Furione, M., Cavicchini, A., Milanesi, G. et al. (1993) Quantification of human cytomegalovirus DNA in peripheral blood polymorphonuclear leukocytes of immunocompromised patients by the polymerase chain reaction. J. Virol. Methods 44, 45-56.