S-[2-(4-Azidosalicylamido)ethylthio]-2-thiopyridine: Radioiodinatable, cleavable, photoactivatible cross-linking agent

Bioconjugate Chemistry

Volumn 7, Issue 3, 1996, Pages 380-384

  Ebright, Yon W ^a   Chen, Yan ^a   Kim, Younggyu ^a   Ebright, Richard H ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOSYNTHESIS; COVALENT BONDS; CROSSLINKING; SULFUR COMPOUNDS;

CARBAMOYL; CROSS LINKING; CROSS LINKING AGENTS; DISULPHIDE BONDS; NEAREST-NEIGHBOUR; PHOTO-CROSS-LINKINGS; PROTEIN DOMAINS; RADIOIODINATION; RELATED COMPOUNDS; SITE-SPECIFIC;

PROTEINS;

CROSS LINKING REAGENT; CYCLIC AMP BINDING PROTEIN; CYSTEINE; DIAGNOSTIC AGENT; DISULFIDE; MERCAPTOETHANOL; PYRIDINE DERIVATIVE; RADIOACTIVE IODINE; S (2 (4 AZIDISALICYLAMIDO)ETHYLTHIO) 2 THIOPYRIDINE; S-(2-(4-AZIDISALICYLAMIDO)ETHYLTHIO)-2-THIOPYRIDINE; SALICYLIC ACID DERIVATIVE;

ARTICLE; AUTORADIOGRAPHY; CHEMISTRY; ESCHERICHIA COLI; PHOTOCHEMISTRY; PHOTOLYSIS; THIN LAYER CHROMATOGRAPHY; TIME; ULTRAVIOLET SPECTROPHOTOMETRY;

AUTORADIOGRAPHY; CHROMATOGRAPHY, THIN LAYER; CROSS-LINKING REAGENTS; CYCLIC AMP RECEPTOR PROTEIN; CYSTEINE; DISULFIDES; ESCHERICHIA COLI; IODINE RADIOISOTOPES; MERCAPTOETHANOL; PHOTOCHEMISTRY; PHOTOLYSIS; PYRIDINES; SALICYLATES; SPECTROPHOTOMETRY, ULTRAVIOLET; TIME FACTORS;

EID: 0030135382     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc9600168     Document Type: Article

References (17)

1. Chong, P., and Hodges, R. (1981) A new heterobifunctional cross-linking reagent for the study of biological interactions between proteins. I. Design, synthesis, and characterization. J. Biol. Chem. 256, 5064-5070.
2. Chong, P., and Hodges, R. (1981) A new heterobifunctional cross-linking reagent for the study of biological interactions between proteins. II. Application to the troponin C-troponin I interaction. J. Biol. Chem. 256, 5071-5076.
3. Zecherle, G., Oleinikov, A., and Traut, R. (1992) The C-terminal domain of Escherichia coli ribosomal protein L7/ L12 can occupy a location near the factor-binding domain of the 50S subunit as shown by cross-linking with N-[4-(p-azidosalicylamido)butyl]-3-(2′-pyridylthio)propionamide. Biochemistry 31, 9526-9532.
4. Chen, Y., Ebright, Y., and Ebright, R. (1994) Identification of the target of a transcription activator protein by protein-protein photocrosslinking. Science 265, 90-92.
5. Wilbur, D. S. (1992) Radiohalogenation of proteins: an overview of radionuclides, labeling methods, and reagents for conjugate labeling. Bioconjugate Chem. 3, 433-470.
6. Schulz, G., and Schirmer, R. (1979) Principles of Protein Structure, Springer-Verlag, New York.
7. Lee, M., Gippert, G., Soman, K., Case, D., and Wright, P. (1989) Three-dimensional solution structure of a single zinc finger DNA-binding domain. Science 245, 635-637.
8. Ebright, Y., Chen, Y., Pendergrast, P. S., and Ebright, R. (1992) Incorporation of an EDTA-metal complex at a rationally selected site within a protein: application to EDTA-iron DNA affinity cleaving with catabolite gene activator protein (CAP) and Cro. Biochemistry 31, 10664-10670.
9. Ji, T., and Ji, I. (1982) Macromolecular photoaffinity labelling with radioactive photoactivable heterobifunctional reagents. Anal. Biochem. 121, 286-289.
10. Fraker, P., and Speck, J. (1978) Protein and cell membrane iodinations with a sparingly soluble chloramide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril. Biochem. Biophys. Res. Commun. 80, 849-857.
11. Shepard, E., de Beer, F., von Holt, C., and Hapgood, J. (1988) The use of sulfosuccinimidyl-2-(p-azidosalicylamido)-1,3′-dithiopropionate as a crosslinking reagent to identify cell surface receptors. Anal. Biochem. 168, 306-313.
12. Zhang, X., Gunasekera, A., Ebright, Y., and Ebright, R. (1991) Derivatives of CAP having no solvent-accessible cysteine residues, or having a unique solvent-accessible cysteine residue at amino acid 2 of the helix-tum-helix motif. J. Biomol. Struct. Dyn. 9, 463-473.
13. Kolb, A., Busby, S., Buc, H., Garges, S., and Adhya, S. (1993) Transcriptional regulation by cAMP and its receptor protein. Annu. Rev. Biochem. 62. 749-795.
14. Ebright, R. (1993) Transcription activation at class I CAP-dependent promoters. Mol. Microbiol. 8, 797-802.
15. Zhou, Y., Zhang, X., and Ebright, R. (1993) Identification of the activating region of CAP: isolation and characterization of mutants of CAP specifically defective in transcription activation. Proc. Natl. Acad. Sci. U.S.A. 90, 6081-6085.
16. Niu, W., Zhou, Y., Dong, Q., Ebright, Y., and Ebright, R. (1994) Characterization of the activating region of Escherichia coli catabolite gene activator protein (CAP): I. Saturation and alanine-scanning mutagenesis. J. Mol. Biol. 243, 595-602.
17. Zhou, Y., Merkel, T., and Ebright, R. (1994) Characterization of the activating region of Escherichia coli catabolite gene activator protein (CAP): II. Role at class I and class II CAP-dependent promoters. J. Mol. Biol. 243, 603-610.