α-Ketoglutarate assay based on fluorescence quenching by NADH

Biotechnology Progress

Volumn 12, Issue 3, 1996, Pages 413-416

  Sharma, Ashutosh ^a   Quantrill, Nigel S M ^a  

^a CRANFIELD UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CALCULATIONS; ENZYMES; ESTERS; FLUORESCENCE; OXIDATION; PHOTOCHEMICAL REACTIONS; SOLUTIONS;

COFACTOR CONVERSION; FLUOROPHORE THIONINE; GLUTAMIC DEHYDROGENASE; HUMAN URINE; KETOGLUTARATE; NICOTINAMIDE ADENINE DINUCLEOTIDE; SUBSTRATE INDUCED QUENCHING;

BIOASSAY;

2 OXOGLUTARIC ACID; ALPHA-KETOGLUTARIC ACID; BUFFER; NICOTINAMIDE ADENINE DINUCLEOTIDE; PHENOTHIAZINE DERIVATIVE; THIONINE; WATER;

ARTICLE; CHEMISTRY; FLUORESCENCE POLARIZATION; HUMAN; SOLUTION AND SOLUBILITY; SPECTROFLUOROMETRY; URINE;

BUFFERS; FLUORESCENCE POLARIZATION; HUMANS; KETOGLUTARIC ACIDS; NAD; PHENOTHIAZINES; SOLUTIONS; SPECTROMETRY, FLUORESCENCE; WATER;

EID: 0030131711     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp960027f     Document Type: Article

References (4)

1. Bergmeyer, H. U. Methods of enzymatic analysis; metabolites 2: tri-and dicarboxylic acids, purines, pyrimidines and derivatives, coenzymes, inorganic compounds, 3rd ed.; 1984; Vol. VII, pp 20-24.
2. Sharma, A. Photolytic oxidation of reduced nicotinamide adenine dinucleotide. Spectrochim. Acta A 1992, 48 (6), 893.
3. Sharma, A.; Quantrill, N. S. On the Substrate Induced Quenching of a fluorophore and its applications in enzyme assay. Proc. SPIE 1994a, 2131, 563.
4. Sharma, A.; Quantrill, N. S. Measurement of ethanol using fluorescence quenching. Spectrochim. Acta A 1994b, 50 (6), 1163.