PCR and sequencing from a single pollen grain

Plant Molecular Biology

Volumn 31, Issue 1, 1996, Pages 189-191

  Petersen, Gitte ^a   Johansen, Bo ^a   Seberg, Ole ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

ITS; PCR; pollen grains; rbcL; sequencing

Indexed keywords

HORDEUM; HORDEUM VULGARE; SECALE CEREALE; SECALE STRICTUM;

EID: 0030130438     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00020620     Document Type: Article

References (11)

1. Asay KH, Hsiao C, Chatterton NJ, Jensen KB, Wang RR: Nucleotide sequence of the internal transcribed spacer region of rDNA in mountain rye, Secale montanum Guss. (Gramineae). Plant Mol Biol 20: 161-162 (1992).
2. Chatterton NJ, Hsiao C, Asay KH, Jensen KB, Wang RR: Nucleotide sequence of the internal transcribed spacer region of rDNA in barley, Hordeum vulgare L. (Gramineae). Plant Mol Biol 20: 165-166 (1992).
3. Gyllensten U, Erlich HA: Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of HLA DQa locus. Proc Natl Acad Sci USA 85: 7652-7656 (1988).
4. Herrmann B, Hummel S (eds): Ancient DNA. Springer-Verlag, New York (1992).
5. Johansen B, Seberg O, Petersen G, Arctander P: Does DAPI detect cytoplasmic DNA? Am J Bot 82: 1215 (1995).
6. Lee SB, Taylor JW: Isolation of DNA from fungal mycelia and single spores. In: Innis M, Gelfand D, Sninsky J, White T (eds) PCR Protocols: A Guide to Methods and Applications, pp. 282-287. Academic Press, San Diego (1990).
7. Long EO, David IB: Repeated genes in eukaryotes. Annu Rev Biochem 49: 727-764 (1980).
8. Saki RK, Gelfand DH, Stoffel S, Scharf ST, Higuchi R, Horn G, Mullis KB, Erlich HA: Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487-491 (1988).
9. Sanger F, Niclen D, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467 (1977).
10. White TJ, Bruns T, Lee S, Taylor J: Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M, Gelfand D, Sninsky J, White T (eds) PCR Protocols: A Guide to Methods and Applications, pp. 315-322. Academic Press, San Diego (1990).
11. Zurawski G, Clegg MT, Brown AHD: The nature of nucleotide sequence divergence between barley and maize chloroplast DNA. Genetics 106: 735-749 (1984).