Rapid Removal of Unincorporated Label and Proteins from DNA Sequencing Reactions

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 5, Issue 2, 1996, Pages 177-180

  Kaczorowski, Tadeusz ^a   Sektas, Marian ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Radiolabeling; Sequenase 2.0; SPEL 6; Wizard resin

Indexed keywords

ADENOSINE TRIPHOSPHATE; DNA; OLIGONUCLEOTIDE; PHOSPHORUS; PROTEIN;

ARTICLE; DNA SEQUENCE; ISOLATION AND PURIFICATION; ISOTOPE LABELING; METHODOLOGY;

ADENOSINE TRIPHOSPHATE; DNA; ISOTOPE LABELING; OLIGONUCLEOTIDES; PHOSPHORUS RADIOISOTOPES; PROTEINS; SEQUENCE ANALYSIS, DNA;

EID: 0030125189     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02789066     Document Type: Article

References (4)

1. Sambrook, J., Fritsch, E. F., and Maniatis T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
2. Kaczorowski, T. and Szybalski, W. (1994) Assembly of 18-nucleotide primers by ligation of three hexamers: sequencing of large genomes by primer walking. Anal. Biochem. 221, 127-135.
3. Kaczorowski, T. and Szybalski, W. (1994) Rapid removal of proteins from DNA sequencing mixtures using a strong cationic exchanger. BioTechniques 17, 34.
4. Kaczorowski, T., Furmanek, B., and Sektas, M. (1994) A method for removal radioactive nucleotides from electrophoretic buffers. BioTechniques 16, 1030,1031.