pH sensitivity of Schizosaccharomyces pombe: Effect on the cellular phenotype associated with lacZ gene expression

Current Genetics

Volumn 29, Issue 5, 1996, Pages 457-461

  Arndt, Greg M ^a   Atkins, David ^a  

^a RW Johnson Pharmaceutical Res I   (Australia)

Author keywords

lacZ; pH sensitivity; Schizosaccharomyces pombe

Indexed keywords

ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES POMBE; SCHIZOSACCHAROMYCES POMBE;

5 BROMO 4 CHLORO 3 INDOLYL BETA GALACTOSIDE; 5-BROMO-4-CHLORO-3-INDOLYL BETA-GALACTOSIDE; BETA GALACTOSIDASE; GALACTOSIDE; INDOLE DERIVATIVE;

ARTICLE; BIOSYNTHESIS; GENE EXPRESSION; GENETICS; LACTOSE OPERON; METABOLISM; PH; PHENOTYPE; SCHIZOSACCHAROMYCES;

BETA-GALACTOSIDASE; GALACTOSIDES; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; INDOLES; LAC OPERON; PHENOTYPE; SCHIZOSACCHAROMYCES;

EID: 0030119243     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/bf02221514     Document Type: Article

References (23)

1. Alfa C, Fantes P, Hyams J, McLeod M, Warbick E (1993) Experiments with fission yeast: a laboratory course manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
2. Arndt GM, Atkins D, Patrikakis M, Izant JG (1995) Gene regulation by antisense RNA in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet 248:293-300
3. Atkins D, Gerlach WL (1994) Artificial ribozyme and antisense gene expression in Saccharomyces cerevisiae. Antisense Res Dev 4:109-117
4. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidmen JG, Smith JA, Struhl K (1987) Current protocols in molecular biology. Green Publishing Associates and Wiley-Interscience, New York
5. Brickman E, Silhavy TJ, Bassford Jr PJ, Shuman HA, Beckwith JR (1979) Sites within gene lacZ of Escherichia coli for formation of active hybrid β-galactosidase molecules. J Bacteriol 139:13-18
6. Bröker M, Bäuml O (1989) New expression vectors for the fission yeast Schizosaccharomyces pombe. FEBS Lett 248:105-110
7. +-ATPase: ion and metabolite transport in the yeast Schizosaccharomyces pombe. In: Nasim A, Young P, Johnson BF (eds) Molecular biology of the fission yeast. Academic Press, San Diego, California, pp 397-429
8. Grimm C, Kohli J, Murray J, Maundrell K (1988) Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker. Mol Gen Genet 215:81-86
9. Guarente L, Ptashne M (1981) Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae. Proc Natl Acad Sci USA 78:2199-2203
10. Kudla B, Persuy MA, Gaillardin C, Heslot H (1988) Construction of an expression vector for the fission yeast Schizosaccharomyces pombe. Nucleic Acids Res 16:8603-8617
11. Larson GP, Itakura K, Ito H, Rossi JJ (1983) Saccharomyces cerevisiae actin-Escherichia coli lacZ gene fusions: synthetic-oligonucleotide-mediated deletion of the 309 base pair intervening sequence in the actin gene. Gene 22:31-39
12. Losson R, Lacroute F (1983) Plasmids carrying the yeast OMP decarboxytase structural and regulatory genes: transcription regulation in a foreign environment. Cell 32:371-377
13. Maundrell K (1990) nmt1 of fission yeast - a highly transcribed gene completely repressed by thiamine. J Biol Chem 265:10857-10864
14. Maundrell K (1993) Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene 123:127-130
15. Moreno S, Klar A, Nurse P (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol 194:795-823
16. Nasim A, Young P, Johnson BF (1989) Molecular biology of the fission yeast. Academic Press, San Diego, California
17. Nocero M, Isshiki T, Yamamoto M, Hoffman CS (1994) Glucose repression of fbp1 transcription in Schizosaccharomyces pombe is partially regulated by adenylate cyclase activation by a G protein a subunit encoded by gpa2 (git8) Genetics 138:39-45
18. Picard D, Schena M, Yamamoto KR (1990) An inducible vector for both fission and budding yeast. Gene 86:257-261
19. Prentice HL (1992) High-efficiency transformation of Schizosaccharomyces pombe by electroporation. Nucleic Acids Res 20:621
20. Prentice HL, Kingston RE (1992) Mammalian promoter element function in the fission yeast Schizosaccharomyces pombe. Nucleic Acids Res 20:3383-3390
21. Rose M, Casadaban MJ, Botstein D (1981) Yeast genes fused to β-galactosidase in Escherichia coli can be expressed normally in yeast. Proc Natl Acad Sci USA 78:2460-2464
22. Rose MD, Winston F, Heiter P (1990) Methods in yeast genetics: a laboratory course manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York
23. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual Cold Spring Harbor Laboratory, Cold Spring Harbor, New York