Recombinant ribosomal P2 protein can unmask anti-ribosomal P autoantibodies from healthy adults

Journal of Laboratory and Clinical Medicine

Volumn 127, Issue 4, 1996, Pages 333-339

  Pan, Zi Jian ^b   Stafford, Haraldine A ^a  

^a OKLAHOMA MEDICAL RESEARCH FOUNDATION   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

AUTOANTIBODY; EPITOPE; HYBRID PROTEIN; IMMUNOGLOBULIN CLASS; IMMUNOGLOBULIN G; PHOSPHOPROTEIN; PHOSPHOPROTEIN P2, RIBOSOMAL; RIBOSOME PROTEIN;

ADULT; AFFINITY CHROMATOGRAPHY; ANTIBODY SPECIFICITY; ARTICLE; BLOOD; FEMALE; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; MALE; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ADULT; ANTIBODY SPECIFICITY; AUTOANTIBODIES; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; HUMANS; IMMUNODOMINANT EPITOPES; IMMUNOGLOBULIN G; IMMUNOGLOBULIN ISOTYPES; MALE; PHOSPHOPROTEINS; RECOMBINANT FUSION PROTEINS; RIBOSOMAL PROTEINS;

EID: 0030115937     PISSN: 00222143     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2143(96)90180-8     Document Type: Article

References (20)

1. Elkon KB, Bonfa E, Brot N. Antiribosomal antibodies in systemic lupus erythematosus. Rheum Dis Clin 1992;18:377-90.
2. Teh LS, Isenberg DA. Antiribosomal P protein antibodies in systemic lupus erythematosus. A reappraisal. Arthritis Rheum 1994;37:307-15.
3. Koren E, Schnitz W, Reichlin M. Concomitant development of chronic active hepatitis and antibodies to ribosomal P proteins in a patient with systemic lupus erythematosus. Arthritis Rheum 1993;36:1325-8.
4. Arnett FC, Reichlin M. Lupus hepatitis: an underrecognized disease feature associated with autoantibodies to ribosomal P. Am J Med 1995;99:465-72.
5. Hulsey M, Golstein R, Scully L, Surbeck W, Reichlin M. Anti-ribosomal P antibodies in systemic lupus erythematosus: a case control study correlating hepatic and renal disease. Clin Immunol Immunopathol 1995;74:252-6.
6. Martin AL, Reichlin M. Fluctuations of antibody to ribosomal "P" proteins correlate with appearance and remission of nephritis in SLE. Lupus (in press).
7. Sato T, Uchiumi T, Ozawa T, Kikuchi M, Nakano M, Arakawa M. Autoantibodies against ribosomal proteins found with high frequency in patients with systemic lupus erythematosus with active disease. J Rheumatol 1991;18:1681-4.
8. Koren E, Koscec M, Reichlin M, Fugate RD, Reichlin MW. Possible role of autoantibodies to ribosomal P-proteins in development of liver disease in patients with systemic lupus erythematosus [Abstract]. Arthritis Rheum 1993;36:S72.
9. Stafford HA, Anderson CJ, Reichlin M. Unmasking of anti-ribosomal P autoantibodies in healthy individuals. J Immunol 1995;155:2754-61.
10. Crow MK, Delgiudice-Asch G, Zehetbauer JB, Lawson JL, Brot N, Weissbach H. Autoantigen-specific T cell proliferation induced by the ribosomal P2 protein in patients with systemic lupus erythematosus. J Clin Invest 1994;94:345-52.
11. Koren E, Reichlin MW, Koscec M, Fugate RD, Reichlin M. Autoantibodies to the ribosomal P proteins react with a plasma membrane-related target on human cells. J Clin Invest 1992;89:1236-41.
12. Tan EM, Cohen AS, Fries JF, et al. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982;25:1271-7.
13. Magsaam J, Gharavi AE, Parnassa AP, Weissbach H, Brot N, Elkon KB. Quantification of lupus anti-ribosome P antibodies using a recombinant P2 fusion protein and determination of the predicted amino acid sequence of the autoantigen in patients' mononuclear cells. Clin Exp Immunol 1989;76:165-71.
14. Hoey T. Expression and purification of lacZ and trpE fusion proteins. In: Ausubel FM, Brent R, Kingston RE, et al., eds. Current protocols in molecular biology. New York: John Wiley and Sons, 1990:16.5.1-6.
15. Moldave K, Sadnik I. Preparation of derived and native ribosomal subunits from rat liver. Methods Enzymol 1979;59: 402-10.
16. Kohsaka H, Yamamoto K, Fujii H, et al. Fine epitope mapping of the human SS-B/La protein. Identification of a distinct autoepitope homologous to a viral gag polyprotein. J Clin Invest 1990;85:1566-74.
17. Itoh Y, Rader MD, Reichlin M. Heterogeneity of the Ro/ SSA antigen and autoanti-Ro/SSA response: evidence of the four antigenically distinct forms. Clin Exp Immunol 1990;81: 45-51.
18. Bonfa E, Llovet R. Elkon K. Immunoblot analysis of IgG subclasses of multiple lupus autoantibodies. J Immunol 1988; 140:2231-6.
19. Avrameas S. Natural autoantibodies: from "horror autotoxicus" to "gnothi seauton". Immunol Today 1991;12:154-9.
20. Friguet B, Chaffotte AF, Djavadi-Ohaniance L, Goldberg ME. Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. J Immunol Methods 1985;77:305-19.