Benzamil applications in cystic fibrosis. Primary culture of nasal epithelial cells as an electrophysiological in vitro model;BENZAMIL UND MUKOVISZIDOSE. PRIMARKULTUR DER NASENSCHLEIMHAUT ALS ELEKTROPHYSIOLOGISCHES IN-VITRO-MODELL

HNO

Volumn 44, Issue 4, 1996, Pages 172-177

  Blank, U ^a   Glanz, H ^a   Eistert, B ^a   Fryen, A ^a   Lindemann, H ^a   Huls G ^a   Clauss, W ^a   Weber, W M ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

amiloride; benzamil; cell culture studies; cystic fibrosis; nasal mucosa

Indexed keywords

AMILORIDE DERIVATIVE; BENZAMIL; SODIUM CHANNEL; AMILORIDE; CHLORIDE CHANNEL; DIURETIC AGENT; DRUG DERIVATIVE; SODIUM;

ARTICLE; CELL CULTURE; CONTROLLED STUDY; CYSTIC FIBROSIS; HUMAN; HUMAN CELL; NOSE MUCOSA; SODIUM ABSORPTION; CELL MEMBRANE POTENTIAL; CULTURE TECHNIQUE; DOSE RESPONSE; DRUG EFFECT; ELECTROLYTE BALANCE; IN VITRO STUDY; METABOLISM; PATHOPHYSIOLOGY; PHYSIOLOGY;

AMILORIDE; CHLORIDE CHANNELS; CULTURE TECHNIQUES; CYSTIC FIBROSIS; DIURETICS; DOSE-RESPONSE RELATIONSHIP, DRUG; HUMANS; MEMBRANE POTENTIALS; NASAL MUCOSA; SODIUM; SODIUM CHANNELS; WATER-ELECTROLYTE BALANCE;

EID: 0030115899     PISSN: 00176192     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Bier H (1993) Die Pathogenese der Sinusitis. HNO 41:A18-A19
2. Bootz F, Reuter G, Giebel W, Galic M (1992) Mukoziliare Aktivität von frei transptantiertem respiratorischem Epithel. HNO 40: 300-305
3. Chinet TC (1994) Use of in vivo nasal transepithelial potential difference to evaluate efficacy in CF gene therapy phase 1 trials. Eur Respir J 7: 1917-1920
4. Chinet TC, Fullton JM, Yankaskas JR, Boucher RC, Stutts MJ (1994) Mechanism of sodium hyperabsorption in cultured cystic fibrosis nasal epithelium: a patch-clamp study. Am J Physiol 266: C1061-C1068
5. Clarke LL, Grubb BR, Yankaskas JR, Cotton CU, McKenzie A, Boucher RC (1994) Relationship of a non-cystic fibrosis transmembrane conductance regulator-mediated chloride conductance to organ-level disease in Cftr(-/-) mice. Proc Natl Acad Sci USA 91: 479-483
6. Deitmer T (1992) Wissenswertes über mukoziliaren Transport. HNO 40: 459-463
7. Gruenert DC, Finkbeiner WE, Widdicombe JH (1995) Culture and transformation of human airway epithelial cells. Am J Physiol 268:L347-L360
8. Kleyman TR, Cragoe EJ (1988) Amiloride and its analogs as tools in the study of ion transport. J Membrane Biol 105: 1-21
9. Knowles MR, Church ML, Waltner WE, Gatzy JT, Boucher RC (1992) Amiloride in cystic fibrosis: safety, pharmacokinetics, and efficacy in the treatment of pulmonary disease. In: Cragoe EJJr, Kleyman TR, Simchowitz L (eds) Amiloride and its analogs. Unique cation transport inhibitors VCH, New York, p 301
10. Knowles MR, Olivier KN, Noone PG, Boucher RC (1995) Pharmacologic modulation of salt and water in the airway epithelium in cystic fibrosis. Am J Respir Crit Care Med 151: 65-69
11. + in cystic fibrosis: is it important? TIPS 16: 120-121
12. Middleton PG, Geddes DM, Alton EWFW (1994) Protocols for in vivo measurement of the ion transport defects in cystic fibrosis nasal epithelium. Eur Respir J 7: 2050-2056
13. Noone PG, Olivier KN, Knowles MR (1994) Modulation of the ionic milieu of the airway in health and disease. Annu Rev Med 45: 421-434
14. Riordan JR, Rommens JM, Kerem B-S, Alon N, Rozmahel R, Grzelczak Z, Zielenski J, Lok S, Plavsic N, Chou J-L, Drumm ML, Iannuzzi MC, Collins FS, Tsui L-C (1989) Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245: 1066-1073
15. Scholte BJ, Kansen M, Hoogeveen AT, Willemse R, Rhim JS, Van Der Kamp AWM, Bijman J (1989) Immortalization of nasal polyp epithelial cells from cystic fibrosis patients. Exp Cell Res 182: 559-571
16. + channels. Annu Rev Physiol 53: 509-530
17. Ussing HH, Zerahn K (1951) Active transport of sodium as a source of electric current in the short-circued isolated frog skin. Acta Physiol Scand 23: 110-127
18. + channel, biophysical properties, pharmacology, ontogenesis, and molecular cloning. Proc Natl Acad Sci USA 91: 247-251
19. + transport across leech skin. Am J Physiol 37:R605-R613
20. Weber W-M, Dannenmaier B, Clauss W (1993) Ion transport across leech integument. J Comp Physiol B 163: 153-159
21. Welsh MJ (1987) Electrolyte transport by airway epithelia. Physiol Rev 67: 1143-1184
22. Widdicombe JH (1994) Accumulation of airway mucus in cystic fibrosis. Pulmon Pharmacol 7: 225-233
23. Yankaskas JR, Cotton CU, Knowles MR, Gatzy JT, Boucher RC (1985) Culture of human nasal epithelial cells on collagen matrix supports. A comparison of bioelectric properties of normal and cystic fibrosis epithelia. Am Rev Respir Dis 132: 1281-1287