Directed Mutagenesis, Ser-56 to Pro, of Bacillus subtilis Phosphatidylserine Synthase Drastically Lowers Enzymatic Activity and Relieves Amplification Toxicity in Escherichia coli

Bioscience, Biotechnology and Biochemistry

Volumn 60, Issue 4, 1996, Pages 630-633

  Saha, Soumitra Kumar ^a   Furukawa, Yutaka ^a   Matsuzaki, Hiroshi ^a   Shibuya, Isao ^a   Matsumoto, Kouji ^a  

^a SAITAMA UNIVERSITY   (Japan)

Author keywords

Bacillus subtilise; Directed mutagenesis; Escherichia coli; Phosphatidylethanolamine; Phosphatidylserine synthase

Indexed keywords

PHOSPHATIDYLSERINE SYNTHASE; PROLINE; SERINE;

ALLELE; AMINO ACID SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

ALLELES; AMINO ACID SEQUENCE; BACILLUS SUBTILIS; CDPDIACYLGLYCEROL-SERINE O-PHOSPHATIDYLTRANSFERASE; CONSERVED SEQUENCE; ESCHERICHIA COLI; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; PROLINE; SERINE;

EID: 0030115872     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.60.630     Document Type: Article

References (26)

1. I. Shibuya. Proa. Lipid Res., 31, 245-299 (1992).
2. A. Ohta and I. Shibuya, J. Bacterial., 132, 434-443 (1977).
3. A. DeChavigny, P. N. Heacock, and W. Dowhan. J. Biol. Client., 266, 5323-5332 (1991).
4. M. Okada, H. Matsuzaki, I. Shibuya, and K. Matsumoto, J, Bacterial., 176, 7456-7461 (1994).
5. A. Dutt and W. Dowhan, J. Bacterial., 147, 535-542 (1981).
6. A. Ohta, K. Waggoner, K. Louie, and W. Dowhan. J. Biol. Cheni., 256. 2219-2225 (1981).
7. C. R. H. Raetz, T. J. Larson, and W. Dowhan. Prae. Natl. Acad. Sei. US A., 74, 1412-1416 (1977).
8. S. K. Saha, S. Nishijima, H. Matsuzaki, I. Shibuya, and K. Matsumoto. Biosei. Biotech. Bioehem., 60. 111-116 (1996).
9. C. Miyazaki, M. Kuroda, A. Ohta, and I. Shibuya. Proc. Natl. Acad. Sei. USA., 82. 7530-7534 (1985).
10. M. Usui, H. Sembongi, H. Matsuzaki, K. Matsumoto, and I. Shibuya. J. Bacterial., 176, 3389-3392 (1994).
11. J. H. Miller, in “Experiments in Molecular Genetics," Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1972.
12. E. Amann, B. Ochs, and K.-J. Abel, Gene, 69. 301-315 (1988).
13. J. W. Dubendorff and F. W. Studier. J. Mol. Biol., 219. 45-59(1991).
14. G. F. Ames, J. Bacterial., 95. 833-843 (1968).
15. I. Shibuya, C. Miyazaki, and A. Ohta, J. Bacterial., 161, 1086-1092 (1985).
16. J. C. Dittmer and R. L. Lester. J. Lipid Res., 5. 126-127 (1964).
17. U. K. Laemmli, Nature (London). 227, 680-685 (1970).
18. J. Kyte and R. F. Doolittle. J. Mol. Biai. 157. 105-132 (1982).
19. J. Gamier, D. J. Osguthorpe, and B. Robson. J. Mat. Biai, 120, 97-120 (1978).
20. R. H. Hjelmstad and R. M. Bell, J. Biai Chem., 265, 1755-1764 (1990).
21. A. Dutt and W. Dowhan, Biochemistry. 24. 1073-1079 (1985).
22. A. S. Gopalakrishnan, Y.-C. Chen, M. Temkin, and W. Dowhan, J. Biai Chem., 261, 1329- 1338 (1986).
23. V. P. Kontinen and H. Tokuda, FEBS Lett., 364. 157-160 (1995).
24. K. Kiyono, K. Miura, Y. Kushima, T. Hikiji, M. Fukushima, I. Shibuya, and A. Ohta. J Bioehem. (Tokyo). 102, 1089-1100 (1987).
25. J. Nikawa, Y. Tsukagoshi, T. Kodaki, and S. Yamashita, Fur. J. Bioehem., 167, 7-12 (1987).
26. J. Nikawa, T. Kodaki, and S. Yamashita, J Biol. Chem., 262, 4876-4881 (1987).