Performance of a slow-scan CCD camera for macromolecular imaging in a 400 kV electron cryomicroscope

Micron

Volumn 27, Issue 2, 1996, Pages 129-139

  Sherman, Michael B ^a   Brink, Jacob ^a   Chiu, Wah ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

400 kV; electron cryomicroscope; modulation transfer function; Slow scan CCD camera; spot scan imaging

Indexed keywords

ANIMALIA; BOS TAURUS;

EID: 0030114748     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/0968-4328(96)00018-2     Document Type: Article

References (41)

1. Brink, J. and Chiu, W., 1991. Contrast analysis of cryo-images of n-paraffin recorded at 400kV out to 2.1Å resolution. J. Microsc. 161, 279-295.
2. Brink, J. and Chiu, W., 1994. Applications of a slow-scan CCD camera in protein electron crystallography. J. Struct. Biol. 113, 23-34.
3. Brink, J., Chiu, W. and Dougherty, M., 1992. Computer-controlled spot-scan imaging of crotoxin complex crystals with 400 keV electrons at near atomic resolution. Ultramicroscopy 46, 229-240.
4. Brink, J., Sherman, M. B. and Chiu, W., 1995. Application of a slow-scan CCD camera in protein electron crystallography at 400 kV. In: Proc. 52nd Ann. Mtg. Microsc. Soc. Amer., Kansas City, MI, Bailey, G. W. and Rider, C. L., (eds). San Francisco Press, pp. 8-9.
5. Chiu, W., 1978. Factors in high resolution biological structure analysis by conventional transmission electron microscopy. Scanning Electron Microscopy 1, 569-580.
6. Chiu, W. and Glaeser, R. M., 1980. Evaluation of photographic emulsions for low-exposure imaging. In: Electron Microscopy at Molecular Dimensions. State of the Art and Strategies for the Future, Baumeister, W. and Vogell, W. (eds). Springer-Verlag, Berlin, pp. 194-199.
7. Crowther, R. A., Henderson, R. and Smith, J. M., 1996. MRC Image Processing Programs. J. Struct. Biol. 116, 9-17.
8. Daberkow, I., Herrmann, K.-H., Liu, L. and Rau, W. D., 1991. Performance of electron image converters with YAG single-crystal screen and CCD sensor. Ultramicroscopy 38, 215-223.
9. de Ruijter, W. J., 1995. Imaging properties and applications of slow-scan charge-coupled device cameras suitable for electron microscopy. Micron 26, 247-276.
10. de Ruijter, W. J. and Weiss, J. K., 1992. Methods to measure properties of slow-scan CCD cameras for electron detection. Rev. Sci. Instrum. 63, 4314-4321.
11. de Ruijter, W. J., Mooney, P. E. and Krivanek, O. L., 1993. Signal transfer efficiency of slow-scan CCD cameras. In: Proc. 51st Ann. Mtg. Micros. Soc. Amer., Cincinnati, OH, Bailey, G. W. and Rieder, C. L. (eds). San Francisco Press, pp. 1062-1063.
12. Dierksen, K., Typke, D., Hegerl, R., Koster, A. J. and Baumeister, W., 1992. Towards automatic electron tomography. Ultramicroscopy 40, 71-87.
13. Dierksen, K., Typke, D., Hegerl, R. and Baumeister, W., 1993. Towards automatic electron tomography II. Implementation of autofocus and low-dose procedures. Ultramicroscopy 49, 109-120.
14. Dierksen, K., Typke, D., Hegerl, R., Walz, J., Sackmann, E. and Baumeister, W., 1995. Three-dimensional structure of lipid vesicles embedded in vitreous ice and investigated by automated electron tomography. Biophys. J. 68, 1416-1422.
15. Downing, K. H. and Grano, D., 1982. Analysis of photographic emulsions for electron microscopy of two-dimensional crystalline specimens. Ultramicroscopy 7, 384-404.
16. Faruqi, A. R., Andrews, H. N. and Raeburn, C., 1994. A large area cooled-CCD detector for electron microscopy. Nucl. Instrum. Meth. Phys. Res. A348, 659-663.
17. Faruqi, A. R., Andrews, H. N. and Henderson, R., 1995. A high sensitivity imaging detector for electron microscopy. Nucl. Instrum. Meth. Phys. Res. A367, 408-412.
18. Frieser, H. and Klein, E., 1958. Properties of emulsions with respect to electron irradiation. Z. Angew. Phys. 10, 337-346.
19. Glaeser, R. M., 1985. Electron crystallography of biological macromolecules. Ann. Rev. Phys. Chem. 36, 243-275.
20. Goodman, J. W., 1968. Introduction to Fourier Optics, Heffner, H. and Siegman, A. E. (eds). McGraw-Hill Book Co., San Francisco.
21. Henderson, R., Baldwin, J. M., Downing, K. H., Lepault, J. and Zemlin, F., 1986. Structure of purple membrane from Halobacterium halobium: recording, measurement and evaluation of electron micrographs at 3.5Å resolution. Ultramicroscopy 19, 147-178.
22. Ishizuka, K., 1993. Analysis of electron image detection efficiency of slow-scan CCD camera. Ultramicroscopy 52, 7-20.
23. Jeng, T. W. and Chiu, W., 1983. Low dose electron microscopy of the crotoxin complex thin crystal. J. Mol. Biol. 164, 329-346.
24. Jeng, T. W., Talmon, Y. and Chiu, W., 1988. Containment system for the preparation of vitrified-hydrated virus specimens. J. Electron Microsc. Tech. 8, 343-348.
25. Koster, A. J. and de Ruijter, W. J., 1992. Practical autoalignment of transmission electron microscopes. Ultramicroscopy 46, 189-197.
26. Koster, A. J., Chen, H., Sedat, J. W. and Agard, D. A., 1992. Automated microscopy for electron tomography. Ultramicroscopy 46, 207-228.
27. Krivanek, O. L. and Mooney, P. E., 1993. Applications of slow-scan CCD cameras in transmission electron microscopy. Ultramicroscopy 49, 95-108.
28. Kujawa, S. and Krahl, D., 1992. Performance of a low-noise CCD camera adapted to a transmission electron microscope. Ultramicroscopy 46, 395-403.
29. Kuo, I. A. M. and Glaeser, R. M., 1975. Development of methodology for low exposure, high resolution electron microscopy of biological specimens. Ultramicroscopy 1, 53-66.
30. Mooney, P. E., Fan, G. Y., Truong, K. V., Bui, D. B. and Krivanek, O. L., 1990. Slow-scan CCD camera for transmission electron microscopy. In: Proc. XIIth Int. Cong. Electron Microsc., Seattle, WA, Peachey, L. D. and Williams, D. B. (eds). San Francisco Press, Inc., pp. 164-165.
31. Mooney, P. E., de Ruijter, W. J. and Krivanek, O. L., 1993. MTF restoration with slow-scan CCD camera. In: Proc. 51st Ann. Mtg. Microsc. Soc. Am., Cincinnati, OH, Bailey, G. W. and Rieder, C. L. (eds). San Francisco Press, pp. 262-263.
32. Mooney, P. E. and Krivanek, O. L., 1994. Image-coupling methods in CCD cameras for electron microscopy. In: Proc. 52nd Ann. Mtg. Microsc. Soc. Am., New Orleans, LA, Bailey, G. W. and Garratt-Reed, A. J. (eds). San Francisco Press, pp. 406-407.
33. Pan, M. and Crozier, P. A., 1993. Quantitative imaging and diffraction of zeolites using a slow-scan CCD camera. Ultramicroscopy 52, 487-498.
34. Schmid, M. F., Dargahi, R. and Tam, M. W., 1993. SPECTRA: a system for processing electron images of crystals. Ultramicroscopy 48, 251-264.
35. Soejima, T., Sherman, M. B., Schmid, M. F. and Chiu, W., 1993. 4 Å projection map of bacteriophage T4 DNA helix-destabilizing protein (gp32*I) crystal by 400-kV electron cryomicroscopy. J. Struct. Biol. 111, 9-16.
36. Spence, J. C. H., 1988. Experimental High-Resolution Electron Microscopy, Oxford University Press, NY.
37. Thomas, I. M. and Schmid, M. F., 1995. A cross-correlation method for merging electron crystallographic image data. J. Microsc. Soc. Amer. 1, 167-173.
38. Unwin, P. N. T., 1975. Beef liver catalase structure: Interpretation of electron micrographs. J. Mol. Biol. 98, 235-242.
39. Unwin, P. N. T. and Henderson, R., 1975. Molecular structure determination by electron microscopy of unstained crystalline specimens. J. Mol. Biol. 94, 425-440.
40. Valentine, R. C., 1966. The response of photographic emulsions to electrons. In: Advances in Optical and Electron Microscopy, Barer, R. and Cosslett, V. E. (eds). Academic Press, New York, pp. 180-203.
41. Zhou, Z. H., Hardt, S., Wang, B., Sherman, M. B., Jakana, J. and Chiu, W., 1996. CTF determination of images of ice-embedded single particles using a graphics interface. J. Struct. Biol. 116, 216-223.