Measurement of Neutrophil Content in Brain and Lung Tissue by a Modified Myeloperoxidase Assay

International Journal of Microcirculation-Clinical and Experimental

Volumn 16, Issue 2, 1996, Pages 89-97

  Kuebler, W M ^a   Abels, C ^a   Schuerer, L ^a   Goetz, A E ^b  

^a Institute for Surgical Resarch   (Germany)

^b LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

Author keywords

Brain; Cobra venom factor; Endotoxin; Ischemia reperfusion; Leukocyte; Lung; Microcirculation; Myeloperoxidase assay; Neutrophil

Indexed keywords

COBROTOXIN; ENDOTOXIN; PEROXIDASE; SNAKE VENOM;

ANIMAL; ARTICLE; BRAIN; CYTOLOGY; ENZYMOLOGY; KINETICS; LEUKOCYTE COUNT; LUNG; MALE; METABOLISM; METHODOLOGY; NEUTROPHIL; PH; RABBIT; RAT; REPERFUSION INJURY; REPRODUCIBILITY; SPRAGUE DAWLEY RAT; VASCULARIZATION;

ANIMALS; BRAIN; COBRA VENOMS; ENDOTOXINS; HYDROGEN-ION CONCENTRATION; KINETICS; LEUKOCYTE COUNT; LUNG; MALE; NEUTROPHILS; PEROXIDASE; RABBITS; RATS; RATS, SPRAGUE-DAWLEY; REPERFUSION INJURY; REPRODUCIBILITY OF RESULTS;

EID: 0030098221     PISSN: 01676865     EISSN: None     Source Type: Journal    
DOI: 10.1159/000179155     Document Type: Article

References (47)

1. Cohnheim J: Über Entzündung und Eiterung. Virchows Arch Pathol Anal Phvsiol 1867;40: 1-79.
2. Kurose I. Granger DN: Evidence implicating xanthine oxidase and neutrophils in reperfu-sion-induced microvascular dysfunction. Ann NY Acad Sei 1994;723:158-179.
3. Heflin AC, Brigham KE: Prevention by granulocyte depletion of increased vascular permeability of sheep lung following endotoxemia. J Clin Invest 1981;68:1253-1260.
4. Andrcwcs FW: Behaviour of the leukocytes in infection and immunity: Lecture II. Lancet 1910;2:8116.
5. 2). Blood 1959;14:303-333.
6. Kueblcr WM. Kuhnlc GEH. Groh J. Goetz AE: Leukocyte kinetics in pulmonary microcirculation: Intravital fluorescence microscopic study. J Appl Physiol 1994:76:65-71.
7. Abels C. Roehrich F. Uhl E. Corvin S. Villrin-gcr A. Dirnagl U. Bacthmann A. Schuercr L: Current evidence on a pathophysiological function of leukocyte/endothelial interactions in cerebral ischemia; in Hartmann A. Yatsu F. Ku-schinsky W (eds): Cerebral Ischemia and Basic Mechanisms. Berlin. Springer. 1994. pp 366-372.
8. Bradley PP. Priebat DA. Christensen RD. Rothstein G: Measurement of cutaneous inflammation: Estimation of neutrophil content with an enzyme marker. J Invest Dermatol 1982:78:206-209.
9. Marklund S: A Simple specific method for the determination of the hemoglobin content of tissue homogenales. Clin Chim Acta 1979:92: 229-234.
10. Bozeman PM. Learn DB. Thomas EL: Assay of the human leukocyte enzymes myeloperoxidase and eosinophil peroxidase. J Immunol Methods 1990:126:125-133.
11. Brown JM, Grosso MA, Terada LS, Bcchler CJ, Toth KM, Whitman GJ, Harken AH, Repine JE: Erythrocytes decrease myocardial hydrogen peroxide levels and reperfusion injury. Am J Physiol 1989:256:H584-H588.
12. Grisham MB. Hernandez LA. Granger DN: Xanthine oxidase and neutrophil infiltration in intestinal ischemia. Am J Physiol 1986;251: G567-G574.
13. Duval DL, Howard D, Mc Calden TA, Billings RE: The delerminalion of myeloperoxidase activity in the liver. Life Sci 1990;47:PL145-PL150.
14. Ormrod DJ, Harrison GL, Miller TF.: Inhibition of neutrophil myeloperoxidase activity by selected tissues. J Pharmacol Methods 1987; 18:137-142.
15. Schierwagcn C, Bvlund-Fellenius AC, Lundberg C: Improved method for quantification of tissue PMN accumulation measured by myeloperoxidase activity. J Pharmacol Methods 1990;23:179-186.
16. Suzuki K. Ota H. Sasagawa S. Sakatani T. Fuji-kura T: Assay method for myeloperoxidase in human polymorphonuclear leukocytes. Anal Biochcm 1983;132:345-352.
17. Mullanc KM, Kraemcr R, Smith B: Myeloperoxidase activity as a quantitative assessment of neutrophil infiltration into ischemic myocardium. J Pharmacol Methods 1985:14:157-167.
18. Cleland WW: The statistical analysis of enzyme kinetic data. Adv Enzvmol Relat Areas Mol Biol 1967:29:1-19.
19. Pulsinelli WA, Brierley JB: A new model of bilateral hemispheric ischemia in the unanesthetized rat. Stroke 1979;10:267-272.
20. Pulsinelli WA, Buchan AM: The four vessel occlusion rat model: Method for complete occlusion of vertebral arteries and control of collateral circulation. Stroke 1988; 19:913-914.
21. Andrews PC. Krinsky NI: The reductive cleavage of myeloperoxidase in half, producing en-zymically active hcmi-myelopcroxidase. J Biol Chem 1981;256:4211-4218.
22. Worthington enzyme manual. Freehold. NJ. Worthington Biochemical Corp. 1972, pp 43-49.
23. Rosen H, Klebanoff SJ: Formation of singlet oxygen by the myeloperoxidase-mediated antimicrobial system. J Biol Chem 1977;252: 4803-4810.
24. Klebanoff SJ: Antimicrobial systems of the polymorphonuclear leukocyte; in Bellanti JA. Dayton DH (eds): Phagocytic cell in host resistance. New York, Raven Press. 1975, pp 45-56.
25. Schultz J. Kaminkcr K: Myeloperoxidase of the leukocyte of normal human blood. I. Content and localization. Arch Biochem Biophys 1962: 96:465-467.
26. Bretz U, Baggliolini M: Biochemical and morphological characterization of azurophilic and specific granules of human neutrophilic polymorphonuclear leukocytes. J Cell Biol I974;63: 251-269.
27. Krawisz JE, Sharon P, Stenson WF: Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in rat and hamster models. Gastroenterology 1984:87:1344-1350.
28. 51Cr-labcled cells and myeloperoxidase. Inflammation 1983:7:247-255.
29. Junod AF: Metabolism, production and release of hormones and mediators in the lung. Am RevRcspir Dis 1975:112:93-108.
30. Murphy S (ed): Astrocytes. Pharmacology and Function. San Diego. Academic Press. 1993.
31. Anderson BO. Brown JM. Shanlcv PF. Bensard DD. Harken AH: Marginating neutrophils are reversiblv adherent to normal lung endothelium. Surgery 1991:109:51-61.
32. Bahrami S, Redl H, Schlag G: Models of endo-toxemia in rodents; in Schlag G, Redl H (eds): Pathophysiology of shock, sepsis, and organ failure. Berlin, Springer. 1993. pp 1019-1030.
33. 5!Cr-labeled red blood cells and RISA. J Appl Physiol 1968:24:79-82.
34. Hogg JC, McLean T, Martin BA, Wiggs B: Erythrocyte transit and neutrophil concentration in the dog lung. J Appl Physiol 1988:65: 1217-1225.
35. Weibel ER: Morphometry of the Human Lung. New York. Academic Press. 1963.
36. Doerschuk CM, Allard MF, Martin BA, Mac-Kenzie A, Autor AP, Hogg JC: Marginated pool of leukocytes in rabbit lungs. J Appl Physiol 1987;63:1806-1815.
37. Saverymuttu SA. Peters AM. Danpure HJ. Reavy HJ. Osman S. Lavender JP: Lung transit of 111-indium labelled granulocytes: relationship to labelling techniques. Scand J Haematol 1983:30:151-160.
38. Forsyth K, Levinsky R: Preparative procedures of cooling and rewarming increase leukocyte integrin expression and function on neutrophils. J Immunol Methods 1990:128:159-163.
39. Worlhcn GS. Schwab B. Elson EL. Downey GP: Mechanics of stimulated neutrophils: Cell stiffening induces retention in capillaries. Science 1989:245:183-186.
40. West JB. Dollery CT. Naimark A: Distribution of blood flow in isolated lung: Relation to vascular and alveolar pressures. J Appl Physiol 1964;19:713-724.
41. Kuebler WM, Kuhnle GEH, Groh J, Sckcll A, Goetz AF.: Alveolar capillary network architecture determines regional leukocyte retention (abstract). Int J Microcirc Clin Exp 1995:14: 357.
42. Groh J. Kuhnle GEH. Ney L. Sckell A. Goetz AE: Effects of isoflurane on regional pulmonary blood flow during one-lung ventilation. Br J Anaesthesia 1995;74:209-216.
43. Kuhnle GEH. Kuebler WM. Groh J. Goetz AE: Effect of blood flow on the leukocyte-cndothc-lium interaction in pulmonary microvessels. Am J Respir Crit Care Med 1995:152:1221-1228.
44. Anderson ML, Smith DS, Nioka S, Subramanian H, Garcia JH, Halsey JH, Chance B: Experimental brain ischemia: Assessment of injury by magnetic resonance spectroscopy and histology. Neurol Res 1990:12:195-204.
45. Barone FC, Hillegass LM, Price WJ, White RF, Lee EV, Feuerstein GZ, Sarau HM, Clark RK, Griswold DE: Polymorphonuclear leukocyte infiltration into cerebral focal ischemic tissue: Myeloperoxidase activity assay and histologic verification. J Neurosci Res 1991:29:336-345.
46. Kiefmann R, Kuhnle G, Kuebler W, Sckell A, Goetz AE: Cobra venom factor induces retention of leukocytes in alveolar capillaries. Int J Microcirc Clin Exp 1994:14:358.
47. Mulligan MS. Polley MJ. Bayer RJ. Nunn MF. Paulson JC. Ward PA: Neutrophil dependent lung injury'. Requirement for P-sclectin (GMP-140) J Clin Invest 1992:90:1600-1607.