Isolation and characterisation of full-length cDNA clones of the giant taro (Alocasia macroirhiza) trypsin/chymotrypsin inhibitor

Plant Molecular Biology

Volumn 30, Issue 5, 1996, Pages 1035-1039

  Mathews, Anne ^a,b   Llewellyn, Danny J ^a,b   Wu, Yingru ^a,b   Dennis, Elizabeth S ^a,b  

^a CSIRO   (Australia)

^b MURDOCH UNIVERSITY   (Australia)

Author keywords

Giant taro; PCR; Proteinase inhibitor; RACE; Trypsin chymotrypsin inhibitor

Indexed keywords

CHYMOTRYPSIN; COMPLEMENTARY DNA; TRYPSIN INHIBITOR;

AMINO ACID SEQUENCE; ARTICLE; DRUG ANTAGONISM; GENETICS; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLANT; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CHYMOTRYPSIN; CLONING, MOLECULAR; DNA, COMPLEMENTARY; MOLECULAR SEQUENCE DATA; PLANTS; SEQUENCE HOMOLOGY, AMINO ACID; TRYPSIN INHIBITORS;

EID: 0030095410     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00020813     Document Type: Article

References (13)

1. Argall ME, Bradbury JH, Shaw DC: Amino-acid sequence of a trypsin/chymotrypsin inhibitor from giant taro (Alocasia macrorrhiza). Biochim Biophys Acta 1204: 189-194 (1994).
2. Bradbury JH, Holloway WD: Chemistry of tropical root crops: significance for nutrition and agriculture in the Pacific. Australian Centre for International Agricultural Research, Canberra, Australia (1988).
3. Chrispeels MJ, Raikhel NV: Short peptide domains target proteins to plant vacuoles. Cell 68: 613-616 (1992).
4. Frohman MA, Dush MK, Martin GR: Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sei USA 85: 8998-9002 (1988).
5. Hammer BC, Shaw DC, Bradbury JH: Trypsin inhibitors from Colocasia esculents, Alocasia macrorrhiza and Cyrtosperma chamissonis. Phytochem 28: 3019-3026 (1989).
6. Hirai M, Nakamura K, Imai T, Sato T: cDNAs encoding for storage proteins in the tubers of taro (Colocasia esculenta Schott). Jpn J Genet 68: 229-236 (1993).
7. Hilder VA, Gatehouse AMR, Sheerman SE, Barker RF, Boulter D: A novel mechanism of insect resistance engineered into tobacco. Nature 330: 160-163 (1987).
8. Johnson R, Narvaez J, An G, Ryan CA: Expression of proteinase inhibitors I and II in transgenic tobacco plants; effects on natural defence against Manduca sexta. Proc Natl Acad Sei USA 86: 871-875 (1990).
9. Luetke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA: Selection of AUG initiation codons differ in plants and animals. EMBO J 6: 43-48 (1987).
10. Lycett GW, Delauney AJ, Croy RRD: Are plant genes different? FEES Lett 153: 43-46 (1983).
11. Ryan CA: Protease inhibitors in plants: genes for improving defenses against insects and pathogens. Annu Rev Phytopath 28: 425-449 (1990).
12. Stiekema WJ, Heidekamp F, Dirkse \VG, van Beckum J, de Haan P, ten Bosch C, Louwerse JD: Molecular cloning and analysis of four potato tuber mRNAs. Plant Mol Biol 11:255-269(1988).
13. Sumathi S, Pattabiraman TN: Natural plant enzyme inhibitors. V. A trypsin/chymotrypsin inhibitor from Alocasia macrorrhiza tuber. Biochim Biophys Acta 485: 167178 (1977).