A one-hybrid system for detecting RNA-protein interactions

Genes to Cells

Volumn 1, Issue 3, 1996, Pages 317-323

  Wilhelm, James E ^a   Vale, Ronald D ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; CAPSID PROTEIN; HYBRID PROTEIN; MESSENGER RNA; N PROTEIN, BACTERIOPHAGE LAMBDA; RNA BINDING PROTEIN; VIRUS PROTEIN;

ARTICLE; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; OPERATOR GENE; REGULATOR GENE; REPORTER GENE; VIRUS CAPSID;

BETA-GALACTOSIDASE; CAPSID; CAPSID PROTEINS; GENES, REPORTER; OPERATOR REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; RNA-BINDING PROTEINS; TERMINATOR REGIONS (GENETICS); TRANSCRIPTION, GENETIC; VIRAL REGULATORY PROTEINS;

EID: 0030089534     PISSN: 13569597     EISSN: 13652443     Source Type: Journal    
DOI: 10.1046/j.1365-2443.1996.25026.x     Document Type: Article

References (29)

1. Brickman E. & Beckwith, J. (1975) Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and phi80 transducing phages. J. Molec. Biol. 96(2), 307-16.
2. Calnan, B.J., Biacalana, S., Hudson, D. & Frankel, A.D. (1991) Analysis of arginine-rich peptides from the HIV Tat protein reveals unusual features of RNA-protein recognition. Genes Dev. 5, 201-210.
3. Cheung, C.T., Niemela, S.L. & Miller, R.H. (1989) One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc. Natl Acad. Sci. USA 86, 2172-2175.
4. Carey, J., Lowary, P.T. & Uhlenbeck, O.C. (1983) Interaction of R17 coat protein with synthetic variants of its ribonucleic acid binding site. Biochemistry 22, 4723-4730.
5. Das, A. (1993) Control of transcription termination by RNAbinding proteins. Annu. Rev. Biochem. 62, 893-930.
6. Franklin, N.C. (1985) Conservation of genome form but not sequence in the transcription antitermination determinants of bacteriophages λ, θ21, and P22. J. Mol. Biol. 181, 75-84.
7. Franklin, N.C. (1989) A plasmid to visualize and assay termination and antitermination of transcription in Escherichia coli. Plasmid 21, 31-42.
8. Franklin, N.C. (1993) Clustered arginine residues of bacteriophage λN protein are essential to antitermination of transcription, but the locale cannot compensate for boxB loop defects. J. Mol. Biol. 231, 343-360.
9. Greenblatt, J. & Li, J. (1981) The NusA gene product of Escherichia coli: its identification and a demonstration that it interacts with the gene N transcription antitermination protein of bacteriophage lambda. J. Mol. Biol. 147, 11-23.
10. Greenblatt, J., Nodwell, J.R. & Mason, S.W. (1993) Transcriptional termination. Nature 364, 401-406.
11. Kjems, J., Calnan, B.J., Frankel, A.D. & Sharp, P.A. (1992) Specific binding of a basic peptide from HIV-1 Rev. EMBO J. 11, 1119-1129.
12. Lazinski,D., Grzadzielska, E. & Das, A. (1989) Sequence-specific recognition of RNA hairpins by bacteriophage antiterminators requires a conserved arginine-rich motif. Cell 59, 207-218.
13. Lowary, P.T. & Uhlenbeck, O.C. (1987) An RNA mutation that increases the affinity of an RNA-protein interaction. Nucl. Acids Res. 15, 10483-10493.
14. MacWilliams, M.P., Celander, D.W. & Gardner, J.F. (1993) Direct genetic selection for a specificRNA-protein interaction. Nucl. Acids Res. 21, 5754-5760.
15. Madhani, H.D. & Guthrie, C. (1994) Multiple roles for U6 snRNA in the splicing pathway. Genes Dev. 8, 1071-86.
16. Mason, S.W., Li, J. & Greenblatt, J. (1992) Host factor requirements for processive antitermination of transcription and suppression of pausing by the N protein of bacteriophage λ. J. Biol. Chem. 267, 19418-19426.
17. McDonald, D., Hope, T.J. & Parslow, T.G. (1992) Posttranscriptional regulation by the human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex proteins through a heterologous RNA binding site. J. Virol. 66, 7232-7238.
18. Miller, J.H. (1992) A Short Course in Bacterial Genetics:A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria, pp. 72-74. Plainview, NY: Cold Spring Harbor Laboratory Press.
19. Nodwell, J.R. & Greenblatt, J. (1991) The nut site of bacteriophage λ is made of RNA and is bound by transcription antitermination factors on the surface of RNA polymerase. Genes Dev. 5, 2141-2151.
20. Nodwell, J.R. & Greenblatt, J. (1993) Recognition of boxA antiterminator RNA by the E. coli antitermination factors NusB and the ribosomal protein S10. Cell 72, 261-268.
21. Romaniuk, P.J., Lowary, P.,Wu, H.N., Stormo, G. & Uhlenbeck, O.C. (1987) RNA binding site of R17 coat protein. Biochemistry 26, 1563-1568.
22. Schneider, D., Tuerk, C. & Gold, L. (1992) Selection of high affinity RNA ligands to the bacteriophage R17 coat protein. J. Mol. Biol. 228, 862-869.
23. Selby, M.J. & Peterlin, B.M. (1990) Trans-activation of Tat via a heterologous RNA binding protein. Cell 62, 769-776.
24. Stripecke, R., Oliveira, C.C., McCarthy, J.E. & Hentze, M.W. (1994) Proteins binding to 50 untranslated region sites: a general mechanism for translational regulation of mRNAs in human and yeast cells. Mol. Cell. Biol. 14, 5898-5909.
25. Tiley, L.S., Madore, S.J., Malim, M.H. & Cullen, B.R. (1992) The VP16 transcription activation domain is functional when targeted to a promoter-proximal RNA sequence. Genes Dev. 6, 2077.
26. Valegard, K., Murray, J.B., Stockley, P.G., Stonehouse, N.J. & Liljas, L. (1994) Crystal structure of an RNA bacteriophage coat protein-operator complex. Nature 371, 623-626.
27. Venkatesan, S., Gerstberger, S.M., Park, H., Holland, S.M. & Nam, Y. (1992) Human immunodeficiency virus type 1 Rev activation can be achieved without Rev-responsive element RNA if Rev is directed to the target as a Rev/MS2 fusion protein which tethers the MS2 operator RNA. J. Virol. 66, 7469-7480.
28. Weeks, K.M., Ampe, C., Schultz, S.C., Steitz, T.A. & Crothers, D.M. (1990) Fragments of HIV-1 Tat protein specifically bind TAR RNA. Science 249, 1281-1285.
29. Whalen, W., Ghosh, B. & Das, A. (1988) NusA protein is necessary and sufficient in vitro for phage λN gene product to suppress a ρ-independent terminator placed downstream of nutL. Proc. Natl Acad. Sci. USA 85, 2494-2498.