Increasing the denaturation temperature during the first cycles of amplification reduces allele dropout from single cells for preimplantation genetic diagnosis

Molecular Human Reproduction

Volumn 2, Issue 3, 1996, Pages 213-218

  Ray, Pierre F ^a   Handyside, Alan H ^a  

^a HAMMERSMITH HOSPITAL   (United Kingdom)

Author keywords

Allele dropout; Polymerase chain reaction; Preimplantation genetic diagnosis; Single cell

Indexed keywords

GLOBIN; PRIMER DNA;

ALLELE; ARTICLE; BETA THALASSEMIA; CYSTIC FIBROSIS; EMBRYO DEVELOPMENT; FEMALE; FERTILIZATION IN VITRO; GENETICS; HETEROZYGOTE; HUMAN; LYMPHOCYTE; METHODOLOGY; MUTATION; PHYSIOLOGY; POLYMERASE CHAIN REACTION; PREGNANCY; REPRODUCIBILITY; TEMPERATURE;

ALLELES; BETA-THALASSEMIA; CYSTIC FIBROSIS; DNA PRIMERS; EMBRYONIC DEVELOPMENT; FEMALE; FERTILIZATION IN VITRO; GLOBINS; HETEROZYGOTE; HUMANS; LYMPHOCYTES; MUTATION; POLYMERASE CHAIN REACTION; PREGNANCY; REPRODUCIBILITY OF RESULTS; TEMPERATURE;

EID: 0030089526     PISSN: 13609947     EISSN: None     Source Type: Journal    
DOI: 10.1093/molehr/2.3.213     Document Type: Article

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