Optimization study of Escherichia coli TB1 cell disruption for cytochrome b5 recovery in a small-scale bead mill

Biotechnology Progress

Volumn 12, Issue 2, 1996, Pages 201-204

  Belo, I ^a   Santos, J A L ^b   Cabral, J M S ^b   Mota, M ^a  

^a UNIVERSITY OF MINHO   (Portugal)

^b INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; ENZYMES; MATHEMATICAL MODELS; OPTIMIZATION; PROTEINS; TOLUENE;

BEAD MILL DISINTEGRATION; CELL DISRUPTION; CELLULAR SUSPENSION CONCENTRATION; CYTOCHROME; LYSOZYME; RESPONSE SURFACE METHODOLOGY; SMALL SCALE BEAD MILL;

CELLS;

CYTOCHROME B5; RECOMBINANT PROTEIN;

ARTICLE; ENZYMOLOGY; ESCHERICHIA COLI; ISOLATION AND PURIFICATION;

CYTOCHROMES B5; ESCHERICHIA COLI; RECOMBINANT PROTEINS;

EID: 0030088489     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp950085l     Document Type: Article

References (21)

1. Baldwin, C. V.; Robinson, C. W. Enhanced Disruption of Candida utilis Using Enzymatic Pretreatment and High-Pressure Homogenization, Biotechnol. Bioeng. 1994, 43, 46-56.
2. Box, G. E. P.; Hunter, W. G.; Hunter, J. S. Statistics for Experimenters. An Introduction to Design, Data Analysis and Model Building; John Wiley & Sons, Inc.: New York, 1978.
3. Bradford, M. A Rapide and Sensitive Method for the Quantification of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. J. Biochem. 1976, 72, 248-254.
4. Currie, J. A.; Dunnill, P.; Lilly, M. D. Release of Protein from Baker's Yeast (Saccharomyces cerevisiae) by Disruption in an Industrial Agitator Mill. Biotechnol. Bioeng. 1972, 14, 725-736.
5. De Smet, M. J.; Kingma, J.; Witholt, B. The Effect of Toluene on the Structure and Permeability of the Outer and Cytoplasmic Membranes of Escherichia coli. Biochim. Biophys. Acta 1978, 506, 64-80.
6. Donald, T. M.; Mao, J. Protein Secretion Systems in Microbial and Mammalian Cells. In Separations Process in Biotechnology, Asenjo, J. A., Ed.; Marcel Dekker: Inc.: New York, 1990; pp 67-94.
7. Félix, H. Permeabilized Cells. Anal. Biochem. 1982, 120, 211-234.
8. Garcia, F. A. P. Cell Wall Disruption. In Recovery Process for Biological Materials; Kennedy, J. F., Cabral, J. M. S., Eds.; J. Wiley: New York, 1993; pp 47-66.
9. Garcia-Ochoa, F.; Santos V. E.; Fritsch, A. P. Nutritional Study of Xanthomonas campestris in Xanthan Gum Production by Factorial Design of Experiments. Enzyme Microb. Technol. 1992, 14, 991-996.
10. Garrido, F.; Banerjee, U. C.; Chisti, Y.; Moo-Young, M. Disruption of a Recombinant Yeast for the Release of β-galactosidase. Bioseparation 1994, 4, 319-328.
11. Goupy, J. La Méthode des Plans d'Experiences, Optimization du Choix des Experiences & de l'Interprétation des Résultats, BORDAS, Paris, 1988.
12. Karim, A.; Kaderbhai, N.; Evans, A.; Harding, V.; Kaderbhai, M. A. Efficient Bacterial Export of a Eukaryotic Cytoplasmic Cytochrome. Bio/Technology 1993, 11, 612-618.
13. Keshavarz, E.; Hoare, M.; Dunnill, P. Biochemical Engineering Aspects of Cell Disruption. In Separations for Biotechnology; Vernall, M. S., Hudson, M. J., Eds.; Ellis Horwood, Ltd.: Chichester, U.K., 1987; pp 62-79.
14. Limon-Lason, J.; Hoare, M.; Orsborn, C. B.; Doyle, D. J.; Dunnill, P. Reactor Properties of a High-Speed Bead Mill for Microbial Cell Rupture. Biotechnol. Bioeng. 1979, 21, 745-774.
15. Philippe, J. Les Méthodes Statistiques en Pharmacie et en Chimie (applications à la recherche à la production et au contrôle) [Statistical Method in Pharmacy and Chemistry (Applications to Research, Production, and Control)]; Masson: Paris, 1967.
16. Rodríguez, M.; Güereca, L.; Valle, F.; Quintero, R.; López-Munguía, A. Penicillin Acylase Extraction by Osmotic Shock. Process Biochem. 1992, 27, 217-223.
17. Santos, J. L.; Belo, I.; Mota, M; Cabral, J. M. S. Freeze/Thawing and Sonication of Escherichia coli TB1 Cells for Cytochrome b5 Recovery. Bioseparation 1996, in press.
18. Sarra, M.; Redin, I.; Ochin, F.; Godia, F.; Casas, C. Application of Factorial Design to the Optimization of Medium Composition in Batch Cultures of Streptomyces lividans TK21 Producing a Hybrid Antibiotic. Biotechnol. Lett. 1993, 15 (6), 559-564.
19. Schütte, H.; Kula, M. Bead Mill Disruption. In Separations Process in Biotechnolology; Asenjo, J. A., Ed.; Marcel Dekker, Inc.: New York, 1990; pp 108-141.
20. van Gaver, D.; Huyghebaert, A. Optimization of a Yeast Cell Disruption with a Newly Designed Bead Mill. Enzyme Microb. Technol. 1990, 13, 665-671.
21. von Bodman, S. B.; Schuler, M. A.; Jollie, D. R.; Sligar, S. G. Synthesis, Bacterial Expression, and Mutagenesis of the Gene Coding for Mammalian Cytochrome b5. Biochemistry 1986, 33, 9443-9447.