Early trypsin, a female-specific midgut protease in Aedes aegypti: Isolation, amino-terminal sequence determination, and cloning and sequencing of the gene

Insect Biochemistry and Molecular Biology

Volumn 26, Issue 2, 1996, Pages 119-126

  Noriega, Fernando G ^a   Wang, Xiao Yu ^a   Pennington, James E ^a   Barillas Mury, Carolina V ^b   Wells, Michael A ^a  

^a University of Arizona ^*  (United States)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

Gene; Mosquito; Trypsin

Indexed keywords

COMPLEMENTARY DNA; PROTEINASE; TRYPSIN;

AEDES; AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; DIGESTIVE SYSTEM; DNA PROBE; ENZYMOLOGY; FEMALE; GENE; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SEQUENCE HOMOLOGY;

AEDES; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DIGESTIVE SYSTEM; DNA PROBES; DNA, COMPLEMENTARY; ENDOPEPTIDASES; FEMALE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENES, INSECT; MOLECULAR SEQUENCE DATA; SEQUENCE HOMOLOGY, NUCLEIC ACID; TRYPSIN;

AEDES AEGYPTI; ANIMALIA;

EID: 0030087932     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/0965-1748(95)00068-2     Document Type: Article

References (18)

1. Barillas-Mury C. V. and Wells M. A. (1993) Cloning and sequencing of the blood meal-induced late trypsin gene from the mosquito Aedes aegypti and characterization of the upstream regulatory region. Insect Molec. Biol. 2, 7-12.
2. Barillas-Mury C. V., Graf R., Hagedorn H. H. and Wells M. (1991) cDNA and deduced amino acid sequence of a blood meal-induced trypsin from the mosquito, Aedes aegypti. Insect Biochem. 21, 825-831.
3. Barillas-Mury C. V., Noriega F. G. and Wells M. A. (1995) Early trypsin activity is part of the signal transduction system that activates transcription of the late trypsin gene in the midgut of the mosquito Aedes aegypti. Insect Biochem. Molec. Biol. 25, 241-246.
4. Benson S. A. and Taylor R. K. (1984) A rapid small-scale procedure for isolation of phage A DNA. BioTechniques 2, 126-127.
5. Felix C. R., Betschart B., Billingsley P. F. and Freyvogel T. A. (1991) Post-feeding induction of trypsin in the midguts of Aedes aegypti. L. (Diptera: Culicidae) is separable into two cellular phases. Insect Biochem. 21, 197-203.
6. Fisk F. W. (1950) Studies on proteolytic digestion in adult Aedes aegypti mosquitoes. Ann. Entomol. Soc. Am. 43, 555-572.
7. Fourney R. M., Miyakoshi J., Day III R. S. and Paterson M. (1988) Northern blotting: efficient RNA staining and transfer. Focus 10, 5-7.
8. Graf R. and Briegel H. (1988) Monoclonal antibodies as probes for Aedes aegypti trypsin. Insect Biochem. 18, 463-470.
9. Graf R. and Briegel H. (1989) The synthetic pathway of trypsin in the mosquito Aedes aegypti L (Diptera: Culicidae) and in vitro stimulation in isolated midguts. Insect. Biochem. 19, 129-137.
10. Henikoff S. (1984) Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28, 351-359.
11. Kalhok S. E., Tabak L. M., Prosser D. E., Brook W., Downe A. E. R. and White B. N. (1993) Isolation, sequencing and characterization of two cDNA clones coding for-trypsin-like enzymes from the midgut of Aedes aegypti. Insect Molec. Biol. 2, 71-79.
12. Kogan P. H. (1990) Substitute blood meal for investigating and maintaining Aedes aegypti (Diptera: Culicidae). J. Med. Entomol. 27, 709-712.
13. Maniatis T., Fritsch E. F. and Sambrook J. (1982) Molecular Cloning: A Laboratory Manual. Cold Springs Harbor Laboratory, Cold Springs Harbor, New York.
14. Neurath H. (1986) Limited proteolysis, domains and the evolution of protein structure. Chemica Scripta 26B, 221-227.
15. Noriega F. G. and Wells M. A. (1993) A comparison of three methods for isolating RNA from mosquitoes. Insect Molec. Biol. 2, 21-24.
16. Noriega F. G., Barillas-Mury C. V. and Wells M. A. (1994) Dietary control of late-trypsin gene transcription in Aedes aegypti. Insect Biochem. Molec. Biol. 24, 627-631.
17. Ohkuma Y., Horikoshi M, Roeder R. G. and Desplan C. (1990) Engrailed, a homeodomain protein, can repress in vitro transcription by competition with the TATA-box-binding protein transcription factor IID. Proc. Natn. Acad. Sci. U.S.A. 87, 2289-2293.
18. Peterson A. M., Barillas-Mury C. V. and Wells M. A. (1994) Sequence of three cDNAs encoding an alkaline midgut trypsin from Manduca sexta. Insect Biochem. Molec. Biol. 24, 463-471.