Expression and characterization of the human erythrocyte anion exchanger in a baculovirus/Sf-9 cell system

Protein Expression and Purification

Volumn 7, Issue 1, 1996, Pages 1-11

  Dale, William E ^a   Textor, Jacquelyn A ^a   Mercer, Robert W ^a   Simchowitz, Louis ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALIA; AUTOGRAPHA CALIFORNICA NUCLEOPOLYHEDROVIRUS; INSECTA; MYTHIMNA UNIPUNCTA; UNIDENTIFIED BACULOVIRUS;

EID: 0030087575     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0001     Document Type: Article

References (46)

1. 1. Knauf, P. A. (1979) Erythrocyte anion exchange and the band 3 protein: Transport kinetics and molecular structure. Curr. Top. Membr. Transp. 12, 249-363.
2. 2. Passow, H. (1986) Molecular aspects of band 3 protein-mediated anion transport across the red blood cell membrane. Rev. Physiol. Biochem. Pharmacol. 103, 61-203.
3. 3. Jennings, M. L. (1985) Kinetics and mechanism of anion transport in red blood cells. Annu. Rev. Physiol. 47, 519-533.
4. 4. Kopito, R. R., and Lodish, H. F. (1985) Primary structure and transmembrane orientation of the murine anion exchange protein. Nature 316, 234-238.
5. 5. Cox, J. V., and Lazarides, E. (1988) Alternative primary structures in the transmembrane domain of the chicken erythroid anion transporter. Mol. Cell. Biol. 8, 1327-1335.
6. 6. Kim, H. R., Yew, N. S., Ansorge, W., Voss, H., Schwager, C., Vennstrom, B., Zenke, M., and Engel, J. D. (1988) Two different mRNAs are transcribed from a single genomic locus encoding the chicken erythrocyte anion transport proteins (band 3). Mol. Cell. Biol. 8, 4416-4424.
7. 7. Lux, S. E., John, K. M., Kopito, R. R., and Lodish, H. F. (1989) Cloning and characterization of band 3, the human erythrocyte anion-exchange protein (AE1). Proc. Natl. Acad. Sci. USA 86, 9089-9093.
8. 8. Tanner, M. J., Martin, P. G., and High, S. (1988) The complete amino acid sequence of the human erythrocyte membrane anion-transport protein deduced from the cDNA sequence. Biochem. J. 256, 703-712.
9. 9. Kopito, R. R. (1990) Molecular biology of the anion exchanger gene family. Int. Rev. Cytol. 123, 177-199.
10. 10. Morgan, M., Hanke, P., Grygorczyk, R., Tintschl, A., Fasold, H., and Passow, H. (1985) Mediation of anion transport in oocytes of Xenopus laevis by biosynthetically inserted band 3 protein from mouse spleen erythroid cells. EMBO J. 4, 1927-1931.
11. 11. Grygorczyk, R., Schwarz, W., and Passow, H. (1987) Potential dependence of the "electrically silent" anion exchange across the plasma membrane of Xenopus oocytes mediated by the band-3 protein of mouse red blood cells. J. Membr. Biol. 99, 127-136.
12. 12. Bartel, D., Lepke, S., Layh-Schmitt, G., Legrum, B., and Passow, H. (1989) Anion transport in oocytes of Xenopus laevis induced by expression of mouse erythroid band 3 protein-encoding cRNA and of a cRNA derivative obtained by site-directed mutagenesis at the stilbene disulfonate binding site. EMBO J. 8, 3601-3609.
13. 13. Zhang, R., Alper, S. L., Thorens, B., and Verkman, A. S. (1991) Evidence from oocyte expression that the erythrocyte water channel is distinct from band 3 and the glucose transporter. J. Clin. Invest 88, 1553-1558.
14. 14. Garcia, A. M., and Lodish, H. F. (1989) Lysine 539 of human band 3 is not essential for ion transport or inhibition by stilbene disulfonates. J. Biol. Chem. 264, 19607-19613.
15. 15. Groves, J. D., and Tanner, M. J. (1992) Glycophorin A facilitates the expression of human band 3-mediated anion transport in Xenopus oocytes. J. Biol. Chem. 267, 22163-22170.
16. 16. Lindsey, A. E., Schneider, K., Simmons, D. M., Baron, R., Lee, B. S., and Kopito, R. R. (1990) Functional expression and subcellular localization of an anion exchanger cloned from choroid plexus. Proc. Natl. Acad. Sci. USA 87, 5278-5282.
17. 17. Fuerstenberg, S., Beug, H., Introna, M., Khazaie, K., Munoz, A., Ness, S., Nordstrom, K., Sap, J., Stanley, I., Zenke, M., and Bennstrom, B. (1990) Ectopic expression of the erythrocyte band 3 anion exchange protein, using a new avian retrovirus vector. J. Virol. 64, 5891-5902.
18. 2+ exchanger in insect cells using a baculovirus vector. J. Biol. Chem. 267, 7828-7833.
19. +/glucose cotransporter in Sf9 cells. Biochim. Biophys. Acta 1104, 151-159.
20. 20. DeTomaso, A. W., Xie, Z. J., Liu, G., and Mercer, R. W. (1993) Expression, targeting, and assembly of functional Na,K-ATPase polypeptides in baculovirus-infected insect cells. J. Biol. Chem. 268, 1470-1478.
21. 21. Piwnica-Worms, H. (1991) Overview of the baculoviral expression system, in "Current Protocols in Molecular Biology" (Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., Eds.), pp. 16.8.1-16.11.7, Wiley, New York.
22. 22. He, X., Wu, X., Knauf, P. A., Tabak, L. A., and Melvin, J. E. (1993) Functional expression of the rat anion exchanger AE2 in insect cells by a recombinant baculovirus. Am. J. Physiol. 264, C1075-C1079.
23. 23. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) "Molecular Cloning: A Laboratory Manual." Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
24. 24. Summers, M. D., and Smith, G. E. (1987) A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agric. Exp. Stn. Bull. 55, 1-56.
25. 25. Hanahan, D. J., and Ekholm, J. E. (1974) The preparation of red cell ghosts (membranes). Methods Enzymol. 31, 168-172.
26. 26. Gallagher, S., Winston, S. E., Fuller, S. A., and Hurrell, J. G. R. (1991) Immunoblotting and immunodetection, in "Current Protocols in Molecular Biology" (Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., Eds.), pp. 10.8.1-10.8.16, Wiley, New York.
27. 27. Jennings, M. L., Anderson, M. P., and Monaghan, R. (1986) Monoclonal antibodies against human erythrocyte band 3 protein. Localization of proteolytic cleavage sites and stilbenedisulfonate-binding lysine residues. J. Biol. Chem. 261, 9002-9010.
28. 28. Grinstein, S., Clarke, C. A., Dupre, A., and Rothstein, A. (1982) Volume-induced increase of anion permeability in human lymphocytes. J. Gen. Physiol. 80, 801-823.
29. 29. Simchowitz, L., Spilberg, I., and De Weer, P. (1982) Sodium and potassium fluxes and membrane potential of human neutrophils: Evidence for an electrogenic sodium pump. J. Gen. Physiol. 79, 453-479.
30. 30. Cotlove, E., Trantham, H. V., and Bowman, R. L. (1958) An instrument and method for automatic, rapid, accurate, and sensitive titration of chloride in biologic samples. J. Lab. Clin. Med. 51, 461-468.
31. 31. Casey, J. R., Pirraglia, C. A., and Reithmeier, R. A. (1992) Enzymatic deglycosylation of human band 3, the anion transport protein of the erythrocyte membrane. Effect on protein structure and transport properties. J. Biol. Chem. 267, 11940-11948.
32. 32. Mueller, T. J., Li, Y. T., and Morrison, M. (1979) Effect of endobeta-galactosidase on intact human erythrocytes. J. Biol. Chem. 254, 8103-8106.
33. 33. Fraser, M. J. (1992) The baculovirus-infected insect cell as a eukaryotic gene expression system. Curr. Top. Microbiol. Immunol. 158, 131-172. [Review]
34. 34. Knauf, P. A. (1989) Kinetics of anion transport, in "The Red Cell Membrane: A Model for Solute Transport" (Raess, B. U., and Tunnicliff, G., Eds.), pp. 171-200, Humana Press, Clifton, NJ.
35. 35. Gunn, R. B., and Kopito, R. R. (1989) Kinetics of band 3-mediated anion transport, in "Anion Transport Protein of the Red Blood Cell Membrane" (Hamasaki, N., and Jennings, M. L., Eds.), pp. 3-14, Elsevier, New York.
36. 36. Cabantchik, Z. I., and Greger, R. (1992) Chemical probes for anion transporters of mammalian cell membranes. Am. J. Physiol. 262, C803-C827. [Review]
37. 37. Knauf, P. A., Fuhrmann, G. F., Rothstein, S., and Rothstein, A. (1977) The relationship between anion exchange and net anion flow across the human red blood cell membrane. J. Gen. Physiol. 69, 363-386.
38. 38. Gunn, R. B. (1978) Considerations of the titratable carrier model for sulfate transport in human red blood cells, in "Membrane Transport Processes" (Hoffman, J. F., Ed.), pp. 61-77, Raven Press, New York.
39. 39. Deuticke, B., von Bentheim, M., Beyer, E., and Kamp, D. (1978) Reversible inhibition of anion exchange in human erythrocytes by an inorganic disulfonate, tetrathionate. J. Membr. Biol. 44, 135-158.
40. 40. Schnell, K. F., Gerhardt, S., and Schoppe-Fredenburg, A. (1977) Kinetic characteristics of the sulfate self-exchange in human red blood cells and red blood cell ghosts. J. Membr. Biol. 30, 319-350.
41. 41. Hughes, R. C., and Butters, T. D. (1981) Glycosylation patterns in cells: An evolutionary marker? Trends Biochem. Sci. 6, 228-230.
42. 42. Sabban, E., Marchesi, V., Adesnik, M., and Sabatini, D. D. (1981) Erythrocyte membrane protein band 3: Its biosynthesis and incorporation into membranes. J. Cell Biol. 91, 637-646.
43. 43. Brahm, J. (1977) Temperature-dependent changes of chloride transport kinetics in human red cells. J. Gen. Physiol. 70, 283-306.
44. 44. Lowe, A. G., and Lambert, A. (1982) Chloride-bicarbonate exchange and related transport processes. Biochim. Biophys. Acta 694, 353-374.
45. 45. Gunn, R. B., and Frohlich, O. (1979) Asymmetry in the mechanism for anion exchange in human red blood cell membranes. Evidence for reciprocating sites that react with one transported anion at a time. J. Gen. Physiol. 74, 351-374.
46. 46. Lee, B. S., Gunn, R. B., and Kopito, R. R. (1991) Functional differences among nonerythroid anion exchangers expressed in a transfected human cell line. J. Biol. Chem. 266, 11448-11454.