Physiological relevance and functional potential of central nervous system-derived cell lines

Molecular Neurobiology

Volumn 12, Issue 1, 1996, Pages 13-38

  Whittemore, Scott R ^a   Snyder, Evan Y ^b  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

Cell lineage; Cell lines; Differentiation; Gene therapy; Neurodegeneration; Progenitor precursor cells; Retroviral transduction; Somatic cell fusion; Stem cells; Targeted oncogenesis; Transgenic mice; Transplantation

Indexed keywords

AGENTS INTERACTING WITH TRANSMITTER, HORMONE OR DRUG RECEPTORS; GROWTH PROMOTOR;

ANIMAL; BIOLOGICAL MODEL; BRAIN; CELL DIFFERENTIATION; CELL LINE; CELL TRANSFORMATION; CENTRAL NERVOUS SYSTEM; CENTRAL NERVOUS SYSTEM DISEASE; CENTRAL NERVOUS SYSTEM TUMOR; CULTURE TECHNIQUE; CYTOLOGY; GENE THERAPY; GENETIC TRANSFECTION; HUMAN; METHODOLOGY; MOUSE; NERVE CELL; PHYSIOLOGY; REVIEW; SPINAL CORD; STEM CELL; SYNAPSE; TRANSGENIC MOUSE;

ANIMALS; BRAIN; CELL DIFFERENTIATION; CELL LINE; CELL TRANSFORMATION, NEOPLASTIC; CENTRAL NERVOUS SYSTEM; CENTRAL NERVOUS SYSTEM DISEASES; CENTRAL NERVOUS SYSTEM NEOPLASMS; CULTURE TECHNIQUES; GENE THERAPY; GROWTH SUBSTANCES; HUMANS; MICE; MICE, TRANSGENIC; MODELS, NEUROLOGICAL; NEURONS; NEUROTRANSMITTER AGENTS; SPINAL CORD; STEM CELLS; SYNAPSES; TRANSFECTION;

EID: 0030079438     PISSN: 08937648     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02740745     Document Type: Article

References (166)

1. Walsh C. (1993) Cell lineage and regional specification in the mammalian neocortex. Perspectives Dev. Neurobiol. 1, 75-80.
2. McKay R. D. G. (1989) The origins of cellular diversity in the mammalian central nervous system. Cell 58, 815-821.
3. Gage F. H., Ray J., and Fisher L. J. (1995) Isolation, characterization, and use of stem cells from the CNS. Ann. Rev. Neurosci. 18, 159-192.
4. McConnell S. K. (1991) The generation of neuronal diversity in the central nervous system. Ann. Rev. Neurosci. 14, 269-300.
5. Luskin M. B. (1994) Neuronal cell lineage in the vertebrate central nervous system. FASEB J. 8, 722-739.
6. Cepko C. L., Ryder E. F., Austin C. P., Walsh C., and Fekete D. M. (1993) Lineage analysis using retrovirus vectors. Meth. Enzymol. 225, 933-960.
7. Cepko C. L. (1989) Immortalization of neural cells via retrovirus-mediated oncogene transduction. Ann. Rev. Neurosci. 12, 47-65.
8. Lendahl U. and McKay R. D. G. (1990) The use of cell lines in neurobiology. Trends Neurosci. 13, 132-137.
9. Whittemore S. R., White L. A., Shihabuddin L. S., and Eaton M. J. (1995) Phenotypic diversity in neuronal cell lines derived from raphe nucleus by retroviral transduction. Methods: Companion Meth. Enzymol. 7, 285-293.
10. Snyder E. Y. (1995) Retroviral vectors for the study of neuroembryology: immortalization of neural cells, in Viral Vectors: Tools for Analysis and Genetic Manipulation of the Nervous System (Kaplit M. G. and Loewy A. D., eds.) Academic, New York, pp. 435-475.
11. Whittemore S. R., Neary J. T., Kleitman N., Sanon H. R., Benigno A., Donahue R. P., and Norenberg M. D. (1994) Isolation and characterization of conditionally immortalized astrocyte cell lines derived from adult human spinal cord. Glia 10, 211-226.
12. Moura Neto V., Mallat M., Chneiweiss H., Premont J., Gros F., and Prochiantz A. (1986) Two simian virus 40 (SV40)-transformed cell lines from the mouse striatum and mesencephalon presenting astrocytic characteristics. I. Immunological and pharmacological properties. Dev. Brain Res. 26, 11-22.
13. Trotter J., Boulter C. A., Sontheimer H., Schachner M., and Wagner E. F. (1989) Expression of v-src arrests murine glial cell differentiation. Oncogene 4, 457-464.
14. Rabotti G. F., Gogusev J., Teutsch B., Mongiat-Lardemer F., and Haguenau F. (1978) Transformation in vitro of glial hamster cells by rous sarcoma virus. J. Natl. Cancer Inst. 60, 113-124.
15. Geller H. M. and Dubois-Dalcq M. (1988) Antigenic and functional characterization of a rat central nervous system-derived cell line immortalized by a retroviral vector. J. Cell Biol. 107, 1977-1986.
16. Evrard C., Galiana E., and Rouget P. (1986) Establishment of 'normal' nervous cell lines after transfer of polyoma virus and adenovirus early genes into murine brain cells. EMBO J. 5, 3157-3162.
17. Galiana E., Borde I., Marin P., Rassoulzadegan M., Cuzin F., Gros F., Rouget P., and Evrard C. (1990) Establishment of permanent astroglial cell lines, able to differentiate in vitro, from transgenic mice carrying the polyoma virus large T gene: an alternative approach to brain cell immortalization. J. Neurosci. Res. 26, 269-277.
18. Major E. O., Miller A. E., Mourrain P., Traub R. G., DeWidt E., and Sever J. (1985) Establishment of a line of human fetal glial cells that supports JC virus multiplication. Proc. Natl. Acad. Sci. USA 82, 1257-1261.
19. Giotta G. J., Heitzmann J., and Cohn M. (1980) Properties of two temperature-sensitive rous sarcoma virus transformed cerebellar cell lines. Brain Res. 202, 445-458.
20. Goodman M. N., Silver J., and Jacobberger J. W. (1993) Establishment and neurite outgrowth properties of neonatal and adult rat olfactory bulb glial cell lines. Brain Res. 619, 199-213.
21. Yoshida T. and Takeuchi M. (1993) Establishment of an astrocyte progenitor cell line: induction of glial fibrillary acidic protein and fibronectin by transforming growth factor-β1. J. Neurosci. Res. 35, 129-137.
22. Kasai H., Fukuda J., and Segawa K. (1987) Transformation of glial cells in mouse embryonic brain cell in vitro with simian virus 40. Neurosci. Lett. 76, 239-244.
23. Jensen N. A., Smith G. M., Garvey J. S., Shine H. D., and Hood L. (1993) Cyclic AMP has a differentiative effect on an immortalized oligodendrocyte cell line. J. Neurosci. Res. 35, 288-296.
24. Verity A. N., Bredesen D., Vonderscher C., Handley V. W., and Campagnoni A. T. (1993) Expression of myelin protein genes and other myelin components in an oligodendrocytic cell line conditionally immortalized with a temperature-sensitive retrovirus. J. Neurochem. 60, 577-587.
25. Russo T., Mogavero A. R., Ammendola R., Mesuraca M., Fiore F., Fatatis A., Salvatore G., and Cimino F. (1993) Immortalization of a cell line showing some characteristics of the oligodendrocyte phenotype. Neurosci. Lett. 159, 159-162.
26. Almazan G. and McKay R. (1992) An oligodendrocyte precursor cell line from rat optic nerve. Brain Res. 579, 234-245.
27. Barnett S. C., Franklin R. J. M., and Blakemore W. F. (1993) In vitro and in vivo analysis of a rat bipotential O-2A progenitor cell line containing the temperature-sensitive mutant gene of the SV40 large T antigen. Eur. J. Neurosci. 5, 1247-1260.
28. Redies C., Lendahl U., and McKay R. G. D. (1991) Differentiation and heterogeneity in T-antigen immortalized precursor cell lines from mouse cerebellum. J. Neurosci. Res. 30, 601-615.
29. Aloisi F., Sun D., Levi G., and Wekerle H. (1990) Establishment of a permanent rat brain-derived glial cell line as a source of purified oligodendrocyte-type 2 astrocyte lineage cell populations. J. Neurosci. Res. 27, 16-24.
30. Trotter J., Crang A. J., Schachner M., and Blakemore W. F. (1993) Lines of glial precursor cells immortalised with a temperature-sensitive oncogene give rise to astrocytes and oligodendrocytes following transplantation into demyelinated lesions in the central nervous system. Glia 9, 25-40.
31. Galiana E., Bernard R., Borde I., Rouget P., and Evrard C. (1993) Proliferation and differentiation properties of bipotent glial progenitor cell lines immortalized with the adenovirus E1A gene. J. Neurosci. Res. 36, 133-146.
32. Louis J. C., Magal E., Muir D., Manthorpe M., and Varon S. (1995) CG-4, a new bipotential glial cell line from rat brain, is capable of differentiating in vitro into either mature oligodendrocytes or type-2 astrocytes. J. Neurosci. Res. 31, 193-204.
33. Bocchini V., Mazzolla R., Barluzzi R., Blasi E., Sick P., and Kettenmann H. (1992) An immortalized cell line expresses properties of activated microglial cells. J. Neurosci. Res. 31, 616-621.
34. Calof A. L. and Guevara J. L. (1993) Cell lines derived from retrovirus-mediated oncogene transduction into olfactory epithelium cultures. Neuroprotocols 3, 222-231.
35. Birren S. J. and Anderson D. J. (1990) A v-myc-immortalized sympathoadrenal progenitor cell line in which neuronal differentiation is initiated by FGF but not NGF. Neuron 4, 189-201.
36. LNGFR, in MAH cells. Science 257, 395-397.
37. Ip N. Y., Nye S. H., Boulton T. G., Davis S., Taga T., Li Y., Birren S. J., Yasukawa K., Kishimoto T., Anderson D. J., Stahl N., and Yancopoulos G. D (1992) CNTF and LIF act on neuronal cells via shared signaling pathways that involve IL-6 signal transducing receptor component gp130. Cell 69, 1121-1132.
38. Ip N. Y., Boulton T. G., Li Y., Verdi J. M., Birren S. J., Anderson D. J., and Yancopoulos G. D. (1994) CNTF, FGF, and NGF collaborate to drive the terminal differentiation of MAH cells into postmitotic neurons. Neuron 13, 443-455.
39. Anderson D. J. (1994) Stem cells and transcription factors in the development of the mammalian neural crest. FASEB J. 8, 707-713.
40. Heath J. K. and Smith A. G. (1988) Regulatory factors of embryonic stem cells. J. Cell Sci. Suppl. 10, 257-266.
41. MacPherson P. A. and McBurney M. W. (1995) P19 embryonal carcinoma cells: a source of cultured neurons amenable to genetic manipulation. Methods: Companion Meth. Enymol. 7, 222-237.
42. Stains W. A., Morassutti D. J., Reuhl K. R., Ally A. I., and McBurney M. W. (1994) Neurons derived from P19 embryonal carcinoma cells have varied morphologies and neurotransmitters. Neuroscience 58, 735-751.
43. Morrassutti D. J., Stains W. A., Magnuson D. S. K., Marshall K. C., and McBurney M. W. (1994) Murine embryonal carcinoma-derived neurons survive and mature following transplantation into adult rat striatum. Neuroscience 58, 753-763.
44. Wojcik B. E., Nothias F., Lazar M., Jouin H., Nicholas J.-F., and Peschanski M. (1993) Catecholaminergic neurons result from intracerebral implantation of embryonal carcinoma cells. Proc. Natl. Acad. Sci. USA 90, 1305-1309.
45. Andrews P. W., Damjanov I., Simon D., Banting G. S., Carlin C., Dracopoli N. C., and Fogh J. (1984) Pluripotent embryonal carcinoma clones derived from human teratocarcinoma cell line Tera-2. Lab. Invest. 50, 147-162.
46. Pleasure S. J., Page C., and Lee V. M.-Y. (1992) Pure, postmitotic, polarized human neurons derived from NTera 2 cells provide a system for expressing exogenous proteins in terminally differentiated neurons. J. Neurosci. 12, 1802-1815.
47. Trojanowski J. Q., Mantione J. R., Lee J. H., Seid D. P., You T., Inge L. J., and Lee V. M.-Y. (1993) Neurons derived from a human teratocarcinoma cell line establish molecular and structural polarity following transplantation into the rodent brain. Exp. Neurol. 122, 283-294.
48. Kleppner S. R., Robinson K. A., Trojanowski J. Q., and Lee V. M.-Y. (1995) Transplanted human neurons derived from a teratocarcinoma cell line (NTera-2) mature, integrate, and survive for over one year in the nude mouse brain. J. Comp. Neurol. 357, 618-632.
49. Masayuki M., Lee V. M.-Y., and Trojanowski J. Q. (1995) Proliferation, cell death and neuronal survival in transplanted human teratocarcinoma (NTera2) cells depend on the graft site in nude and SCID mice. Lab. Invest. 73, 1-14.
50. Ronnett G. V., Hester L. D., Nye J. S., Connor K., and Snyder S. H. (1990) Human cortical neuronal cell line: establishment from a patient with unilateral megalencephaly. Science 248, 603-605.
51. Poltorak M., Isono M., Freed W. J., Ronnett G. V., and Snyder S. H. (1992) Human cortical neuronal cell line (HCN-1): further in vitro characterization and suitability for brain transplantation. Cell Transplant. 1, 3-15.
52. Truckenmiller M. E., Kulaga H., Coggiano M., Wyatt R., Snyder S. H., and Sweetnam P. M. (1993) Human cortical neuronal cell line: a model for HIV-1 infection in an immature neuronal system. AIDS Res. Human Retroviruses 9, 445-453.
53. Mizachi Y., Rodriguez I., Sweetnam P. M., Rubinstein A., and Volsky D. J. (1994) HIV type 1 infection of human cortical neuronal cells: enhancement by select neuronal growth factors. AIDS Res. Human Retroviruses 10, 1593-1596.
54. White L. A., Keane R. W., and Whittemore S. R. (1994) Differentiation of an immortalized CNS neuronal cell line decreases their susceptibility to cytotoxic T lymphocyte cell lysis in vitro. J. Neuroimmunol. 49, 135-143.
55. Sorieul S. and Ephrussi B. (1961) Karylogical demonstration of hybridization of mammalian cells in vitro. Nature 190, 653-654.
56. Pontecorvo G. (1975) Production of mammalian somatic cell hybrids by means of polyethylene glycol treatment. Som. Cell Genet. 1, 397-400.
57. Lee H. J., Hammond D. N., Large T. H., Roback J. D., Sim J. A., Brown D. A., Otten U. H., and Wainer B. H. (1990) Neuronal properties and trophic activities of immortalized hippocampal cells from embryonic and young adult mice. J. Neurosci. 10, 1779-1767.
58. Cashman N. R., Durham H. D., Blusztajn J. K., Oda K., Tabira T., Shar I. T., Dahrouge S., and Antel J. P. (1992) Neuroblastoma X spinal cord (NSC) hybrid cell lines resemble developing motor neurons. Devel. Dynam. 194, 209-221.
59. Choi H. K., Won L. A., Kontur P. J., Hammond D. N., Fox A. P., Wainer B. H., Hoffmann P. C., and Heller A. (1991) Immortalization of embryonic mesencephalic dopaminergic neurons by somatic cell fusion. Brain Res. 552, 67-76.
60. Smith R. G., Alexianu M. E., Crawford G., Nyormoi O., Stefani E., and Appel S. H. (1994) Cytotoxicity of immunoglobulins from amyotrophic lateral sclerosis patients on a hybrid motoneuron cell line. Proc. Natl. Acad. Sci. USA 91, 3393-3397.
61. Choi H. K., Won L., Roback J. D., Wainer B. H., and Heller A. (1992) Specific modulation of dopamine expression in neuronal hybrid cells by primary cells from different brain regions. Proc. Natl. Acad. Sci. USA 89, 8943-8947.
62. Crawford G. D. Jr., Le W.-D., Smith R. G., Xie W.-J., Stefani E., and Appel S. H. (1992) A novel N18TG2 X mesencephalon cell hybrid expresses properties that suggest a dopaminergic cell line of substantia nigra origin. J. Neurosci. 12, 3392-3398.
63. Blusztajn J. K., Venturini A., Jackson D. A., Lee H. J., and Wainer B. H. (1992) Acetylcholine synthesis and release is enhanced by dibutyryl cyclic AMP in a neuronal cell line derived from mouse septum. J. Neurosci. 12, 793-799.
64. Hammond D. N., Wainer B. H., Tonsgard J. H., and Heller A. (1986) Neuronal properties of clonal hybrid cell lines derived from central cholinergic neurons. Science 234, 1237-1240.
65. Lee H. J., Hammond D. N., Large T. H., and Wainer B. H. (1990) Immortalized young adult neurons from the septal region: generation and characterization. Dev. Brain Res. 52, 219-228.
66. Eves E. M., Marsden K. M., Downen M., Sheman L., Rosner M. R., and Wainer B. H. (1993) A novel hybrid immortalization strategy yields a more mature neuronal phenotype. Absts. Am. Soc. Neurosci. 19, 243.
67. Botteri F. M., van der Putter H., Wong D. F., Sauvage C. A., and Evans R. M. (1987) Unexpected thymic hyperplasia in transgenic mice harboring a neuronal promoter fused with simian virus 40 large T antigen. Mol. Cell. Biol. 7, 3178-3184.
68. Efrat S., Teitelman G., Anwar M., Ruggiero D., and Hanahan D. (1988) Glucagon gene regulatory region directs oncoprotein expression to neurons and pancreatic α cells. Neuron 1, 605-613.
69. Baetge E. E., Behringer R. R., Messing A., Brinster R. L., and Palmiter R. D. (1988) Transgenic mice express the human phenylethanolamine N-methyltransferase gene in adrenal medulla and retina. Proc. Natl. Acad. Sci. USA 85, 3648-3652.
70. Hammang J. P., Baetge E. E., Behringer R. R., Brinster R. L., Palmiter R. D., and Messing A. (1990) Immortalized retinal neurons derived from SV40 T-antigen-induced tumors in transgenic mice. Neuron 4, 775-782.
71. Mandel G. and McKinnon D. (1993) Molecular basis of neural-specific gene expression. Ann. Rev. Neurosci. 164, 323-345.
72. Mellon P. L., Windle J. J., Goldsmith P. C., Padula C. A., Roberts J. L., and Weiner R. I. (1990) Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis. Neuron 5, 1-10.
73. Martinez de la Escalera G., Choi A. L., and Weiner R. I. (1992) Beta 1-adrenergic regulation of the GT1 gonadotrophin-releasing hormone (GnRH) neuronal cell lines: stimulation of GnRH release via receptors positively coupled to adenylate cyclase. Endocrinology 131, 1397-1402.
74. Martinez de la Escalera G., Gallo F., Choi A. L., and Weiner R. I. (1992) Dopaminergic regulation of the GT1 gonadotrophin-releasing hormone (GnRH) neuronal cell lines: stimulation of GnRH release via D1-receptors positively coupled to adenylate cyclase. Endocrinology 131, 2965-2971.
75. Milenkovic L., D'Angelo G., Kelly P. A., and Weiner R. I. (1994) Inhibition of gonadotropin hormone-releasing hormone release by prolactin from GT1 neuronal cell lines through prolactin receptors. Proc. Natl. Acad. Sci. USA 91, 1244-1247.
76. Martinez de la Escalera G., Choi A. L. H., and Weiner R. I. (1994) Biphasic GABAergic regulation of GnRH secretion in GT1 cell lines. Neuronal Reg. 59, 420-425.
77. Wetsel W. C., Eraly S. A., Whyte D. B., and Mellon P. L. (1993) Regulation of gonadotropin-releasing hormone by protein kinase-A and -C in immortalized hypothalamic neurons. Endocrinology 132, 2360-2370.
78. Mellon P. L., Eraly S. A., Belsham D. D., Lawson M. A., Clark M. E., Whyte D. B., and Windle J. J. (1995) An immortal cell culture model of hypothalamic gonadotropin-releasing hormone neurons. Methods: Companion Meth. Enzymol. 7, 303-310.
79. Miller G. M., Silverman A.-J., Roberts J. L., Dong K. W., and Gibson M. J. (1993) Functional assessment of intrahypothalamic implants of immortalized gonadotropin-releasing hormone-secreting cells in female hypogonadal mice. Cell Transplant. 2, 251-257.
80. Suri C., Fung B. P., Tischler A. S., and Chikaraishi D. M. (1993) Catecholaminergic cell lines from the brain and adrenal glands of tyrosine hydroxylase-SV40 T antigen transgenic mice. J. Neurosci. 13, 1280-1291.
81. Largent B. L., Sosnowski R. G., and Reed R. R. (1993) Directed expression of an oncogene to the olfactory neuronal lineage in transgenic mice. J. Neurosci. 13, 300-312.
82. b-tsA58 transgenic mouse. Proc. Natl. Acad. Sci. USA 88, 5096-5100.
83. b-tsA58 transgenic mouse hippocampal neuroepithelial cells. Neuroreport 5, 2197-2200.
84. Eves E. M., Kwon J., Downen M., Tucker M. S., Wainer B. H., and Rosner M. R. (1994) Conditional immortalization of neuronal cells from postmitotic cultures and adult CNS. Brain Res. 656, 396-404.
85. Kilpatrick T. J. and Bartlett P. F. (1995) Cloned multipotential precursors from the mouse cerebrum require FGF-2, whereas glial restricted precursors are stimulated with either FGF-2 or EGF. J. Neurosci. 15, 3563-3661.
86. Cattaneo E. and McKay R. (1990) Proliferation and differentiation of neuronal stem cells regulated by nerve growth factor. Nature 347, 762-765.
87. Deloulme J. C., Baudier J., and Sensenbrenner M. (1991) Establishment of pure neuronal cultures from fetal rat spinal cord and proliferation of the neuronal precursor cells in the presence of fibroblast growth factor. J. Neurosci. Res. 29, 499-509.
88. Ray J. and Gage F. H. (1994) Spinal cord neuroblasts proliferate in response to basic fibroblast growth factor. J. Neurosci. 14, 3548-3564.
89. Murphy M., Drago J., and Bartlett P. F. (1990) Fibroblast growth factor stimulates the proliferation and differentiation of neural precursors in vitro. J. Neurosci. Res. 25, 463-475.
90. Ray J., Peterson D. A., Schinstine M., and Gage F. H. (1993) Proliferation, differentiation, and long-term culture of primary hippocampal neurons. Proc. Natl. Acad. Sci. USA 90, 3602-3606.
91. Vicario-Abejon C., Johe K. K., Hazel T. G., Collazo D., and McKay R. D. G. (1995) Functions of basic fibroblast growth factor and neurotrophins in the differentiation of hippocampal neurons. Neuron 15, 105-114.
92. DeHamer M. K., Guevera J. L., Hannon K., Olwin B. B., and Calof A. L. (1994) Genesis of olfactory receptor neurons in vitro: regulation of progenitor cell divisions by fibroblast growth factors. Neuron 13, 1083-1097.
93. Reynolds B. A., Tetzlaff W., and Weiss S. (1992) A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes. J. Neurosci. 12, 4565-4574.
94. Davis A. A. and Temple S. (1994) A self-renewing multipotential stem cell in embryonic rat cerebral cortex. Nature 372, 263-266.
95. Reynolds B. A. and Weiss S. (1992) Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255, 1707-1710.
96. Frederiksen K. and McKay R. D. G. (1988) Proliferation and differentiation of rat neuroepithelial precursor cells in vivo. J. Neurosci. 8, 1144-1151.
97. Richards L. J., Kilpatrick T. J., and Bartlett P. F. (1992) De novo generation of neuronal cells from the adult mouse brain. Proc. Natl. Acad. Sci. USA 89, 8591-8595.
98. Kilpatrick T. J. and Bartlett P. F. (1993) Cloning and growth of multipotential neural precursors: requirements for proliferation and differentiation. Neuron 10, 255-265.
99. Temple S. and Davis A. A. (1994) Isolated rat cortical progenitor cells are maintained in division in vitro by membrane-associated factors. Development 120, 999-1008.
100. Drago J., Murphy M., Carrol S. M., Harvey R. P., and Bartlett P. F. (1991) Fibroblast growth factor-mediated proliferation of central nervous system precursors depends on endogenous production of insulin-like growth factor. Proc. Natl. Acad. Sci. USA 88, 2199-2203.
101. Drago J., Nurcombe V., Pearse M. J., Murphy M., and Bartlett P. F. (1991) Basic fibroblast growth factor upregulates steady state levels of laminin B1 and B2 chain mRNA in cultured neuroepithelial cells. Exp. Cell Res. 196, 246-254.
102. Vescovi A. L., Reynolds B. A., Fraser D. D., and Weiss S. (1993) bFGF regulates the proliferative fate of unipotent (neuronal) and bipotent (neuronal/astroglial) EGF-generated CNS progenitor cells. Neuron 11, 951-966.
103. Ahmed S., Reynolds B. A., and Weiss S. (1995) BDNF enhances the differentiation but not the survival of CNS stem cell-derived neuronal precursors. J. Neurosci. 15, 5765-5778.
104. Ghosh A. and Greenberg M. E. (1995) Distinct roles for bFGF and NT-3 in the regulation of cortical neurogenesis. Neuron 15, 89-103.
105. Guillemot F., Lo L.-C., Johnson J. E., Auerbach A., Anderson D. J., and Joyner A. L. (1993) Mammalian achaete-scute homolog 1 is required for the early development of olfactory and autonomic neurons. Cell 75, 463-476.
106. Lee J. E., Hollenberg S. M., Snider L., Turner D. L., Lipnick N., and Weintraub H. (1995) Conversion of Xenopus ectoderm into neurons by NeuroD, a basic helix-loop-helix protein. Science 12, 836-843.
107. Gage F. H., Coates P. W., Palmer T. D., Kuhn H. G., Fisher L. J., Suhonen J. O., Peterson D. A., Suhr S. T., and Ray J. (1995) Survival and differentiation of adult neuronal progenitor cells transplanted to the adult brain. Proc. Natl. Acad. Sci. USA 92, 11,879-11,883.
108. Ray J., Fisher L. J., Kuhn H. G., Peterson D. A., Tuszynski M., and Gage F. H. (1994) Neuroblasts cultured from embryonic hippocampus and spinal cord can survive and express neural markers after grafting in the adult CNS. Absts. Am. Soc. Neurosci. 20, 670.
109. Nishikawa R., Xiang-Dong J., Harmon R. C., Lazar C. S., Gill G. N., Cavenee W. K., and Su Huang J. S. (1994) A mutant epidermal growth factor receptor common in human gliomas confers enhanced tumorigenicity. Proc. Natl. Acad. Sci. USA 91, 7727-7731.
110. Schlegel J., Merdes A., Stumm G., Albert F. K., Forsting M., Hynes N., and Kiessling M. (1994) Amplification of the epidermal-growth-factor-receptor gene correlates with different growth behaviour in human glioblastoma. Int. J. Cancer 56, 72-77.
111. Rolelj S., Weinberg R. A., Fanning P., and Klagsbrun M. (1988) Basic fibroblast growth factor fused to a signal peptide transforms cells. Nature 331, 173-175.
112. Calof A. L., ed. (1993) Neuroprotocols, vol. 3: Immortalizing Neural Cells. Academic, San Diego, CA.
113. Cepko C. L. (1992) Transduction of genes using retrovirus vectors, in Current Protocols in Molecular Biology (Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., and Struhl K., eds.), Wiley, New York, pp. 9.10.1-9.14.3.
114. Miller D. G., Adam M. A., and Miller A. D. (1990) Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection. Mol. Cell. Biol. 10, 4239-4242.
115. Bartlett P. F., Reid H. H., Bailey K. A., and Bernard O. (1988) Immortalization of mouse neural precursor cells by the c-myc oncogene. Proc. Natl. Acad. Sci. USA 85, 3255-3259.
116. Murphy M., Bernard O., Reid K., and Bartlett P. F. (1990) Cell lines derived from mouse neural crest are representative of cells at various stages of differentiation. J. Neurobiol. 22, 522-535.
117. Bernard O., Reid H. H., and Bartlett P. F. (1992) Role of the c-myc and the N-myc proto-oncogenes in the immortalization of neural precursors. J. Neurosci. Res. 24, 9-20.
118. Ryder E. F., Snyder E. Y., and Cepko C. L. (1990) Establishment and characterization of multipotent neural cell lines using retrovirus vector-mediated oncogene transfer. J. Neurobiol. 21, 356-375.
119. Eves E. M., Tucker M. S., Roback J. D., Downen M., Rosner M. R., and Wainer B. H. (1992) Immortal rat hippocampal cell lines exhibit neuronal and glial lineages and neurotrophin gene expression. Proc. Natl. Acad. Sci. USA 89, 4373-4377.
120. Giordano M., Takashima H., Herranz A., Poltorak M., Geller H. M., Marone M., and Freed W. J. (1993) Immortalized GABAergic cell lines derived from rat striatum using a temperature-sensitive allele of the SV40 large T antigen. Exp. Neurol. 124, 395-400.
121. Pessac B., Girard A., Romey G., Crisanti P., Lorinet A. M., and Calothy G. (1983) A neuronal clone derived from a rous sarcoma virus-transformed quail embryo neuroretina established culture. Nature 302, 616-618.
122. DeVitry F., Camier M., Czernichow P., Benda P. H., Cohen P., and Tixier-Vidal A. (1974) Establishment of a clone of mouse hypothalamic neurosecretory cells synthesizing neurophysin and vasopressin. Proc. Natl. Acad. Sci. USA 71, 3575-3579.
123. Frederiksen K., Jat P. S., Valtz N., Levy D., and McKay R. (1988) Immortalization of precursor cells from the mammalian CNS. Neuron 1, 439-448.
124. Evrard C., Borde I., Marin P., Galiana E., Prèmont F., Gros F., and Rouget P. (1990) Immortalization of bipotential and plastic glio-neuronal precursor cells. Proc. Natl. Acad. Sci. USA 87, 3062-3066.
125. Mehler M. F., Rozental R., Dougherty M., Spray D. C., and Kessler J. A. (1993) Cytokine regulation of neuronal differentiation of hippocampal progenitor cells. Nature 362, 62-65.
126. Soula C., Foulquier F., Duprat A. M., and Cochard P. (1993) Lineage analysis of early neural plate cells: cells with purely neuronal fate coexist with bipotential neuroglial progenitors. Dev. Biol. 159, 196-207.
127. Turner D. L. and Cepko C. L. (1987) A common progenitor for neurons and glia persists in rat retina late in development. Nature 328, 131-136.
128. Leber S. M., Breedlove S. M., and Sanes J. R. (1990) Lineage, arrangement, and death of clonally related motoneurons in chick spinal cord. J. Neurosci. 10, 2451-2462.
129. Turner D. L., Snyder E. Y., and Cepko C. L. (1990) Lineage-independent determination of cell type in the embryonic mouse retina. Neuron 4, 833-845.
130. White L. A., Eaton M. J., Castro M. C., Klose K. J., Globus M. Y., Shaw G., and Whittemore S. R. (1994) Distinct regulatory pathways control neurofilament expression and neurotransmitter synthesis in immortalized serotonergic neurons. J. Neurosci. 14, 6744-6753.
131. Eaton M. J., Staley J. K., Globus M. Y.-T., and Whittemore S. R. (1995) Developmental regulation of early serotonergic neuronal differentiation: the role of brain-derived neurotrophic factor and membrane depolarization. Dev. Biol. 170, 169-182.
132. Eaton M. J. and Whittemore S. R. (1995) ACTH activation of adenylate cyclase in raphe neurons: multiple regulatory pathways control serotonergic neuronal differentiation. J. Neurobiol. 28, 465-481.
133. Azmitia E. C. and de Kloet E. R. (1987) ACTH neuropeptide stimulation of serotonergic neuronal maturation in tissue culture: modulation by hippocampal cells, in Progress in Brain Research (de Kloet E. R., Wiegant V. M., and de Wied D., eds.), Elsevier, Amsterdam, pp. 311-318.
134. Ramaekers F., Rigter H., and Leonard B. E. (1978) Parallel changes in behavior and hippocampal monoamine metabolism in rats after administration of ACTH analogues. Pharmacol. Biochem. Behav. 8, 547-551.
135. Kitchens D. L., Snyder E. Y., and Gottlieb D. I. (1994) FGF and EGF are mitogens for immortalized neural progenitors. J. Neurobiol. 25, 797-807.
136. Kornblum H. I., Raymon H. K., Morrison R. S., Cavanaugh K. P., Bradshaw R. A., and Leslie F. M. (1990) Epidermal growth factor and basic fibroblast growth factor: effects on an overlapping population of neocortical neurons in vitro. Brain Res. 535, 255-263.
137. Whittemore S. R. and White L. A. (1993) Target regulation of neuronal differentiation in a temperature-sensitive cell line derived from medullary raphe. Brain Res. 615, 27-40.
138. Gao W.-Q. and Hatten M. E. (1994) Immortalizing oncogenes subvert the establishment of granule cell identity in developing cerebellum. Development 120, 1059-1070.
139. Snyder E. Y. (1994) Grafting immortalized neurons to the CNS. Curr. Opin. Neurobiol. 4, 742-751.
140. Suhr S. and Gage F. H. (1994) Gene therapy for neurological disorders. Trends Genet. 10, 210-214.
141. Snyder E. Y. (1996) Use of non-neuronal cells for gene delivery. Neuroreport (in press).
142. Renfranz P. J., Cunningham M. G., and McKay R. D. G. (1991) Region-specific differentiation of the hippocampal stem cell line HiB5 upon implantation into the developing mammalian brain. Cell 66, 713-729.
143. Snyder E. Y., Deitcher D. L., Walsh C., Arnold-Aldea S., Hartwieg E. A., and Cepko C. L. (1992) Multipotent neural cell lines can engraft and participate in development of mouse cerebellum. Cell 66, 33-51.
144. Shihabuddin L. S., Hertz J. A., Holets V. R., and Whittemore S. R. (1995) The adult CNS retains the potential to direct region-specific differentiation of a transplanted neuronal precursor cell line. J. Neurosci. 15, 6666-6678.
145. Shihabuddin L. S., Brunschwig J.-P., Holets V. R., Bunge M. B., and Whittemore S. R. (1996) Induction of mature neuronal properties in immortalized neuronal precursor cells following grafting in the neonatal CNS. J. Neurocytol. 25, 101-111.
146. Snyder E. Y., Yandava B. D., Pan Z.-H., Yoon C., and Macklis J. D. (1993) Immortalized posrnatally-derived cerebellar progenitors can engraft and participate in the development of multiple structures at multiple stages along the mouse neuraxis. Absts. Am. Soc. Neurosci. 19, 613.
147. Onifer S. M., Whittemore S. R., and Holets V. R. (1993) Variable morphological differentiation of a raphe-derived neuronal cell line following transplantation into the adult rat CNS. Exp. Neurol. 122, 130-142.
148. Macklis J. D. (1993) Transplanted neocortical neurons migrate selectively into regions of neuronal degeneration produced by chromophore-targeted laser photolysis. J. Neurosci. 13, 3848-3863.
149. Sheen V. L. and Macklis J. D. (1994) Apoptotic mechanisms in targeted photolytic neuronal cell death by chromophore-activated photolysis. Exp. Neurol. 130, 67-81.
150. Macklis J. D., Yoon C. H., and Snyder E. Y. (1994) Immortalized neural progenitors differentiate toward repletion of a neuronal population selectively eliminated from adult mouse neocortex by targeted photolysis. Exp. Neurol. 127, 9.
151. Gage F. H., Kawaja M. D., and Fisher L. J. (1991) Genetically modified cells: applications for intracerebral grafting. Trends Neurosci. 14, 328-333.
152. Glorioso J. C., Goins W. F., Meany C. A., Fink D. A., and DeLuca N. A. (1994) Gene transfer to brain using herpes simplex virus vectors. Ann. Neurol. 35, S28-S34.
153. Freese A., Geller A. I., and Neve R. (1990) HSV-1 vector mediated neuronal gene delivery. Biochem. Pharmacol. 40, 2189-2199.
154. Le Gal La Salle G., Robert J. J., Berrard S., Ridous V., Stratford-Perricaudet L. D. Perricaudet M., and Mallet J. (1993) An adenovirus vector for gene transfer into neurons and glia in the brain. Science 259, 988-990.
155. Anton R., Kordower J. H., Maidment N. T., Manaster J. S., Kane D. J., Rabizadeh S., Schueller S. B., Yang J., Edwards R. H., Markham C. H., and Bredesen D. E. (1994) Neural-targeted gene therapy for rodent and primate hemiparkinsonism. Exp. Neurol. 127, 207-218.
156. Snyder E. Y., Taylor R. M., and Wolfe J. H. (1995) Neural progenitor cell engraftment corrects lysosomal storage throughout the MPS VII mouse brain. Nature 374, 367-370.
157. Lacorazza H. D., Flax J. D., Snyder E. Y., and Jendoubi M. (1996) Expression of human β-hexosaminidase α-subunit gene (the gene defect of Tay Sachs disease) in mouse brains upon engraftment of transduced progenitor cells. Nat. Med. 2, 424-429.
158. Martinez-Serrano A., Lundberg C., Horellou P., Fischer W., Bentiage C., Campbell K., McKay R. D. G., Mallet J., and Björklundt A. (1995) CNS-derived neural progenitor cells for gene transfer of nerve growth factor to the adult brains complete rescue of axotomized cholinergic neurons after transplantation into the septum. J. Neurosci. 15, 5668-5680.
159. Martinez-Serrano A., Fischer W., and Björklund A. (1995) Reversal of age-dependent cognitive impairments and cholinergic neuron atrophy by NGF-secreting neural progenitors grafted to the basal forebrain. Neuron 15, 473-484.
160. Snyder E. Y. and Flax J. D. (1995) Transplantation of neural progenitors and stem-like cells as a strategy for gene therapy and repair of neurodegenerative diseases. Mental Retard. Dev. Disabil. Res. Rev. 1, 27-38.
161. Wright W. E., Pereira-Smith O. M., and Shay J. W. (1989) Reversible cellular senescence: implications for immortalization of normal human diploid fibroblasts. Mol. Cell. Biol. 9, 3088-3092.
162. Stewart N. and Bacchetti S. (1991) Expression of SV40 large T antigen, but not small t antigen, is required for the induction of chromosomal aberrations in transformed human cells. Virology 180, 49-57.
163. Ray F. A., Peabody D. S., Cooper J. L., Cram I. S., and Kraemer P. M. (1990) SV40 antigen alone drives karyotype instability that precedes neoplastic transformation of human diploid fibroblasts. J. Cell Biochem. 42, 13-31.
164. Wainwright M. S., Perry B. D., Won L. A., O'Malley K. L., Wang W.-Y., Ehrlich M. E., and Heller A. (1995) Immortalized murine striatal neuronal cell lines expressing dopamine receptors and cholinergic properties. J. Neurosci. 15, 676-688.
165. Bottenstein J. E. and Sato G. H. (1979) Growth of a rat neuroblastoma cell line in serum-free supplemented media. Proc. Natl. Acad. Sci. USA 76, 514-517.
166. Rudge J. S., Eaton M. J., Mather P., Lindsay R. M., and Whittemore S. R. (1996) CNTF induces raphe neuronal precursors to switch from a serotonergic to a cholinergic phenotype in vitro. Mol. Cell Neurosci., in press.