The PRINS technique: Potential use for rapid preimplantation embryo chromosome screening

Molecular Human Reproduction

Volumn 2, Issue 2, 1996, Pages 135-138

  Pellestor, Franck ^a   Girardet, Anne ^a   Andréo, Brigitte ^a   Lefort, Geneviève ^b   Charlieu, Jean Paul ^c  

^a Centre National de la Recherche Scientifique   (France)

^b Hopital Saint Charles   (France)

^c WESTERN GENERAL HOSPITAL   (United Kingdom)

Author keywords

Human pre embryo; In situ labelling; Oligonucleotide; Preimplantation diagnosis; PRINS

Indexed keywords

FLUORESCENT DYE; PRIMER DNA; SATELLITE DNA;

ARTICLE; BLASTOCYST; BLASTOMERE; CHROMOSOME ABERRATION; CHROMOSOME DISORDER; CYTODIAGNOSIS; CYTOLOGY; FERTILIZATION IN VITRO; GENETIC MARKER; GENETIC SCREENING; HUMAN; HUMAN CHROMOSOME; IN SITU HYBRIDIZATION; METHODOLOGY; POLYMERASE CHAIN REACTION; ULTRASTRUCTURE;

BLASTOCYST; BLASTOMERES; CHROMOSOME ABERRATIONS; CHROMOSOME DISORDERS; CHROMOSOMES, HUMAN; CYTODIAGNOSIS; DNA PRIMERS; DNA, SATELLITE; FERTILIZATION IN VITRO; FLUORESCENT DYES; GENETIC MARKERS; GENETIC SCREENING; HUMANS; IN SITU HYBRIDIZATION; POLYMERASE CHAIN REACTION;

EID: 0030072677     PISSN: 13609947     EISSN: None     Source Type: Journal    
DOI: 10.1093/molehr/2.2.135     Document Type: Article

References (18)

1. Audibert, F., Hedon, B., Humeau, C., Arnal, F., Mares, P. et al. (1989) Results of in-vitro fertilization attempts in patients with unexplained infertility. Hum. Reprod., 4, 766-771.
2. Bottema, C.D.K. and Sommer, S.S. (1993) PCR amplification of specific alleles: rapid detection of known mutations and polymorphisms. Mutat. Res., 288, 93-102.
3. Edirisinghe, W.R., Murch, A.R. and Yovich, J.L. (1992) Cytogenetic analysis of human oocytes and embryos in an in-vitro fertilization programme. Hum. Reprod., 7, 230-236.
4. Gosden, J. and Lawson, D. (1994) Rapid chromosome identification by oligonucleotide-primed in-situ DNA synthesis (PRINS). Hum. Mol. Genet., 7, 431-436.
5. Gosden, J. and Lawson, D. (1995) Instant PRINS: a rapid method for chromosome identification by detecting repeated sequences in situ. Cytogenet. Cell Genet., 68, 57-60.
6. Handyside, A. H., Kontogianni, E.H., Hardy, K. and Winston, R.M.L. (1990) Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature, 244, 768-770.
7. Harper, J.C., Coonen, E., Ramaekers, F.C.S., Delhanty, J.D.A., Handyside, A.H. et al. (1994) Identification of the sex of human preimplantation embryos in two hours using an improved spreading method and fluorescent in-situ hybridization (FISH) using directly labelled probes. Hum. Reprod., 9, 721-724.
8. Koch, J., Kolvraa, S., Petersen, K., Gregersen, N. and Bolund, L. (1989) Oligonucleotide-priming methods for the chromosome-specific labelling of alpha satellite DNA in situ. Chromosoma, 98, 259-265.
9. Michaeli, G., Fejgin, M., Ghetler, Y., Ben Nun, I., Beyth, Y. and Amiel, A. (1990) Chromosomal analysts of unfertilized oocytes and morphologically abnormal preimplantation embryos from an in vitro fertilization program. J. In Vitro Fertil. Embryo Transfer, 7, 341-346.
10. Munné, S., Lee, A., Rosenwaks Z., Grifo, J. and Cohen, J. (1993) Diagnosis of major chromosome aneuploidies in human preimplantation embryos. Hum. Reprod., 8, 2185-2191.
11. Munné, S., Grifo, J., Cohen, J. and Weier, H.U.G. (1994) Chromosome abnormalities in human arrested preimplantation embryos: a multiple-probe FISH study. Am. J. Hum. Genet., 56, 150-159.
12. Pellestor, A., Girardet, A., Andreo, B. and Charlieu, J.P. (1994a) A polymorphic alpha satellite sequence specific for human chromosome 13 detected by oligonucleotide Primed In-situ labelling (PRINS). Hum. Genet., 94, 346-348.
13. Pellestor, F., Dufour, M.C., Arnal, F. and Humeau., C. (1994b) Direct assessment of the rate of chromosomal abnormalities in grade IV human embryos produced by in-vitro fertilization procedure. Hum. Reprod., 9, 293-302.
14. Pellestor, F., Girardet, A., Lefort, G., Andreo, B. and Charlieu, J.P. (1995a) Use of the primed in-situ labelling (PRINS) technique for a rapid detection of chromosomes 13, 16, 18, 21, X and Y. Hum. Genet., 95, 12-17.
15. Pellestor, F., Girardet, A., Lefort, G., Andreo, B. and Charlieu, J.P. (1995b) Selection of chromosome specific primers and their use in simple and double PRINS techniques for rapid in-situ identification of human chromosomes. Cytogenet. Cell Genet., 70, 138-142.
16. Pellestor, F., Girardet, A., Lefort, G., Andreo, B. and Charlieu, J.P. (1995c) PRINS as a method for rapid chromosomal labeling on human spermatozoa. Mol. Reprod. Dev., 40, 333-337.
17. Pellestor, F., Quenesson, I., Coignet, L., Girardet, A., Andreo, B. et al. (1996) FISH and PRINS, a strategy for rapid chromosome screening: application to the assessment of aneuploidy in human sperm. Cytogenet. Cell Genet., in press.
18. Willard, H.F. and Waye, J.S. (1987) Hierarchical order in chromosome-specific human alpha satellite DNA. Trends Genet., 3, 192-198.