Monoclonal antibodies against unfertilized zona-free mouse oocytes: Characterization and effects on fertilization

Molecular Reproduction and Development

Volumn 43, Issue 1, 1996, Pages 47-54

  Jin, Meishan ^a,b   Larsson, Anders ^a   Nilsson, B Ove ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b UPPSALA UNIVERSITY   (Sweden)

Author keywords

Complement activation; Contraception; In vitro fertilization; Intrasplenic immunization; Monoclonal antibodies; Oocyte lysis

Indexed keywords

CELL ANTIBODY; MONOCLONAL ANTIBODY;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; COMPLEMENT ACTIVATION; CONTRACEPTION; CONTROLLED STUDY; FEMALE; FERTILIZATION IN VITRO; HAMSTER; HUMAN; HUMAN CELL; MALE; MOUSE; NONHUMAN; OOCYTE; PRIORITY JOURNAL; SPERM; ZONA PELLUCIDA;

ANIMALS; ANTIBODIES; ANTIBODIES, MONOCLONAL; ANTIGENS, SURFACE; BINDING SITES, ANTIBODY; CELL FUSION; CELL SURVIVAL; CRICETINAE; CROSS REACTIONS; FEMALE; FERTILIZATION; FERTILIZATION IN VITRO; HUMANS; INSEMINATION; MALE; MICE; MICE, INBRED STRAINS; OOCYTES; SPECIES SPECIFICITY; SPERM-OVUM INTERACTIONS; SPERMATOZOA; SUPEROVULATION; ZONA PELLUCIDA;

ANIMALIA; CRICETINAE;

EID: 0030071684     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2795(199601)43:1<47::AID-MRD6>3.0.CO;2-U     Document Type: Article

References (21)

1. Belew M, Juntti N, Larsson A, Porath J (1987): A one-step purification method for monoclonal antibodies based on salt-promoted adsorption chromatography on a "thiophilic" adsorbent. J Immunol Methods 102:173-182.
2. Boldt J, Wolf DP (1986): An improved method for isolation of fertile zona-free mouse eggs. Gamete Res 13:213-222.
3. Boue F, Lassalle B, Duquenne C, Villaroya S, Testart J, Lefevre A, Finaz C (1992): Human sperm proteins with testicular and epididymal origin that participate in fertilization: Modulation of sperm binding to zona-free hamster oocytes, using monoclonal antibodies. Mol Reprod Dev 33 470-480.
4. Bronson RA, Fusi F, Cooper GW, Phillips DM (1990): Antisperm antibodies induce polyspermy by promoting adherence of human sperm to zona-free hamster eggs. Hum Reprod 5:690-696
5. Doekes G, van Es LA, Daha MR (1984). Binding and activation of the first complement component by soluble immune complexes: Effect of complex size and composition. Scand J Immunol 19:99-110.
6. Jin M, Nilsson OB (1992): Enhanced production of monoclonal antibodies against zona pellucida-free, unfertilized mouse oocytes by intrasplenic immunization. Hybridoma 11:689-699.
7. Jin M, Johansson L, Sundström P, Nilsson BO (1994): Characterization of mouse-hamster-human cross-reacting anti-oocyte monoclonal antibodies produced by intrasplenic immunization of mice with twelve zona-free mouse oocytes. Mol Reprod Dev 37:446-451.
8. Jin M, Larsson A, Nilsson BO (1989): A functionally active complement system is present in uterine secretion of the mouse prior to implantation Am J Reprod Immunol 26 53-57.
9. Karp LE, Williamson RA, Moor DE, Shy KK, Plymate SR, Smith WD (1981): Sperm penetration assay: Useful test in evaluation of male fertility. Obstet Gynecol 57:620-623.
10. Lachman PJ (1991): The control of homologous lysis. Immunol Today 12:312-315
11. Larsson A, Sjöquist J (1989): Binding of complement components C1q, C3, C4 and Co to a model immune complex in ELISA. J Immunol Methods 119:103-109.
12. Law SKA, Reid KBM (1988): "Complement." Oxford: IRL Press Ltd, pp 9-27.
13. Liang LF, Dean J (1990): Oocyte development: Molecular biology of the zona pellucida. Vitam Horm 47:115-159.
14. Martin RH (1993): Detection of genetic damage in human sperm. Reprod Toxicol 7:47-52.
15. Miller GW, Nussenzweig VA (1975): A new complement function: Solubilization of antigen-antibody aggregates. Proc Nat Acad Sci 2:418-422.
16. Morgan BP (1989): Complement membrane attack on nucleated cells: resistance, recovery and non-lethal effects. Biochem J 246:1-14.
17. Myles DG (1993): Molecular mechanisms of sperm-egg membrane binding and fusion in mammals. Dev Biol 158:35-45.
18. Naito K, Toyota Y, Yanagimachi R (1992): Production of normal mice from oocytes fertilized and developed without zonae pellucidae. Hum Reprod 7:281-285.
19. Nilsson BO. Larsson A (1990): Intrasplenic immunization with minute amounts of antigen. Immunol Today 11:10-12.
20. Nilsson BO, Svalander PC, Larsson A (1987): Immunization of mice and rabbits by intrasplenic deposition of nanogram quantities of protein attached to Sepharose beads or nitrocellulose paper strips. J Immunol Methods 99:67-75.
21. Porter RR, Reid KBM (1979): Activation of the complement system by antibody-antigen complexes: the classical pathway. Adv Protein Chem 33:1-64.