MTS interferon assay: A simplified cellular dehydrogenase assay for interferon activity using a water-soluble tetrazolium salt

Journal of Interferon and Cytokine Research

Volumn 16, Issue 1, 1996, Pages 31-33

  Khabar, Khalid S A ^a   Al Zoghaibi, Fahad ^a   Dzimiri, Maud ^a   Taha, Mohammed ^a   Al Tuwaijri, Ali ^b   Al Ahdal, Mohammed N ^a  

^a KING FAISAL SPECIALIST HOSPITAL AND RESEARCH CENTER   (Saudi Arabia)

^b KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

[No Author keywords available]

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; 5 (3 CARBOXYMETHOXYPHENYL) 3 (4,5 DIMETHYL 2 THIAZOLYL) 2 (4 SULFOPHENYL) 2H TETRAZOLIUM; BOVINE SERUM ALBUMIN; CRYSTAL VIOLET; FORMAZAN; INTERFERON; OXIDOREDUCTASE; PHENAZINE METHOSULFATE; RECOMBINANT ALPHA2A INTERFERON; TETRAZOLIUM; UNCLASSIFIED DRUG;

AMNION CELL; ANIMAL CELL; ARTICLE; ASSAY; CELL DAMAGE; CELL STRAIN L 929; CONTROLLED STUDY; CYTOPATHOGENIC EFFECT; ENCEPHALOMYOCARDITIS VIRUS; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; OPTICAL DENSITY; PRIORITY JOURNAL; SOLUBILITY;

ANIMALIA; BOVINAE; ENCEPHALOMYOCARDITIS VIRUS; VIOLA (ANGIOSPERM);

EID: 0030071339     PISSN: 10799907     EISSN: None     Source Type: Journal    
DOI: 10.1089/jir.1996.16.31     Document Type: Article

References (6)

1. MEAGER, A. (1987). Quantification of interferons by antiviral assays and their standardization. In: Lymphokines and Interferons. M.J. Clements, A.G. Morris, and A.J.H. Gearing (eds.) Oxford: IRL Press, pp. 129-147.
2. FINTER, N.B. (1969). Dye uptake methods of assessing viral cytopathogenicity and their application to interferon assays. J. Gen. Virol. 5, 419-425.
3. BERG, K., HANSEN, M.B., and NIELSEN, S.E. (1990). A new sensitive bioassay for precise quantification of interferon activity as measured via the mitochondrial dehydrogenase function in cells (MTT-method). AMPIS 98, 156-162.
4. ARMSTRONG, J.A. (1980). Cytopathic effect inhibition assay for interferon: microculture plate assay. Methods Enzymol. 78, 381-387.
5. CORY, A.H., OWEN, T.C., BARLTROP, J.A., and CORY, J.G. (1991). Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. Cancer Commun. 3, 207-212.
6. ABU-KHABAR, K.S., ARMSTRONG, J.A., and HO, M. (1992). Analysis and examination of cytokine interactions by the median-effect model: an example with antiviral action of tumor necrosis factor and interferon-γ. J. Interferon Res. 12, 156-165.