Investigation on minor degraded derivatives of the recombinant hirudin variant HM2 from Hirudinaria manillensis isolated by isoelectric focusing in multicompartment electrolyzers

Electrophoresis

Volumn 17, Issue 5, 1996, Pages 932-937

  Bossi, Alessandra ^a   Righetti, Pier Giorgio ^a   Visco, Carlo ^b   Breme, Umberto ^b   Mauriello, Maurizio ^b   Valsasina, Barbara ^b   Orsini, Gaetano ^b   Wenisch, Elisabeth ^c  

^a UNIVERSITY OF MILAN   (Italy)

^b PFIZER INC   (United States)

^c Univ of Agric and Forestry BOKU   (Austria)

Author keywords

Hirudin; Immobilized pH gradients; Isoelectric focusing; Mass spectra; Proteolysis

Indexed keywords

ELECTROLYTIC CELLS; ELECTROPHORESIS; FOCUSING; MASS SPECTROMETERS; MASS SPECTROMETRY; PROTEOLYSIS; PROTEINS;

AMINO-ACIDS; CLEAVAGE PRODUCTS; ELECTROLYZERS; HIRUDIN; IMMOBILIZED PH GRADIENT; ISOELECTRIC FOCUSING; MASS SPECTRA; MINOR COMPONENTS; PH GRADIENTS; PI VALUES;

AMINO ACIDS;

LEPIRUDIN;

CONFERENCE PAPER; CONTROLLED STUDY; DRUG CONTAMINATION; DRUG IMPURITY; DRUG MANUFACTURE; DRUG PURITY; ESCHERICHIA COLI; ISOELECTRIC FOCUSING; LEECH; MASS SPECTROMETRY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEIN VARIANT; RECOMBINANT DNA TECHNOLOGY; TECHNIQUE;

EID: 0030070955     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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