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Purification to homogeneity and characterization of acyl coenzyme A:6-aminopenicillanic acid acyltransferase of P. chrysogenum
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The isopenicillin N acyltransferase of P. chrysogenum has isopenicillin N amidohydrolase, 6-aminopenicillanic acid acyltransferase and penicillin amidase activities, all of which are encoded by the single penDE gene
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Alvarez E, Meesschaert B, Montenegro E, Gutiérrez S, Díez B, Barredo JL, Martín JF (1993) The isopenicillin N acyltransferase of P. chrysogenum has isopenicillin N amidohydrolase, 6-aminopenicillanic acid acyltransferase and penicillin amidase activities, all of which are encoded by the single penDE gene. Eur J Biochem 215:323-332
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Cloning, sequence analysis and transcriptional study of the isopenicillin N synthase of Penicillium chrysogenum AS-P-78
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Cloning and characterization of acyl-CoA:6-APA acyltransferase gene of Penicillium chrysogenum
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Barredo JL, Solingen P van, Díez B, Alvarez E, Cantoral JM, Kattevilder A, Smaal EB, Groenen MAM, Veenstra AE, Martín JF (1989b) Cloning and characterization of acyl-CoA:6-APA acyltransferase gene of Penicillium chrysogenum. Gene 83:291-300
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Large amplification of a 35-kb DNA fragment carrying two penicillin biosynthetic genes in high penicillin producing strains of Penicillium chrysogenum
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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Two genes involved in penicillin biosynthesis are linked in a 5.1 kb Sa/I fragment in the genome of Penicillium chrysogenum
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Díez B, Barredo JL, Alvarez E, Cantoral JM, Solingen P van, Groenen MAM, Veenstra AE, Martín JF (1989) Two genes involved in penicillin biosynthesis are linked in a 5.1 kb Sa/I fragment in the genome of Penicillium chrysogenum. Mol Gen Genet 218:572-576
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The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the α-aminoadipyl-cysteinyl-valinesynthetase and linkage to the pchC and penDE genes
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Díez B, Gutiérrez S, Barredo JL, Solingen P van, Voort LHM van der, Martín F (1990) The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the α-aminoadipyl-cysteinyl-valinesynthetase and linkage to the pchC and penDE genes. J Biol Chem 265:16358-16365
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Fierro F, Gutiérrez S, Díez B, Martín JF (1993) Resolution of four large chromosomes in penicillin-producing filamentous fungi: the penicillin gene cluster is located on chromosome II (9.6 mb) in Penicillium notatum and chromosome I (10.4 mb) in Penicillium chrysogenum. Mol Gen Genet 241:573-579
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Gutiérrez S, Díez B, Montenegro E, Martín JF (1991) Characterization of the Cephalosporium acremonium pchAB gene encoding α-aminoadipyl-cysteinyl-valine synthetase, a large multidomain peptide synthetase: linkage to the pcbC gene as a cluster of early cephalosporin-biosynthetic genes and evidence of multiple functional domains. J Bacteriol 173:2354-2365
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Resolution of chromosomes III and VI of Aspergillus nidulans by pulsed-field gel electrophoresis shows that the penicillin biosynthetic pathway genes pcbAB, pcbC, and penDE are clusterd on chromosome VI (3.0 megabases)
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Montenegro E, Fierro F, Fernández FJ, Gutiérrez S, Martín JF (1992) Resolution of chromosomes III and VI of Aspergillus nidulans by pulsed-field gel electrophoresis shows that the penicillin biosynthetic pathway genes pcbAB, pcbC, and penDE are clusterd on chromosome VI (3.0 megabases). J Bacteriol 174:7063-7067
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