Mechanical perturbation elicits a phenotypic difference between Dictyostelium wild-type cells and cytoskeletal mutants

Biophysical Journal

Volumn 70, Issue 2 I, 1996, Pages 1054-1060

  Eichinger, Ludwig ^a   Köppel, Bernd ^b   Noegel, Angelika A ^b   Schleicher, Michael ^a   Schliwa, Manfred ^a   Weijer, Kees ^a   Witke, Walter ^c   Janmey, Paul A ^c  

^a UNIVERSITY OF MUNICH   (Germany)

^b MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^c BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA ACTININ; CYTOSKELETON PROTEIN; F ACTIN;

ARTICLE; CELL MUTANT; CYTOSKELETON; DICTYOSTELIUM DISCOIDEUM; NONHUMAN; PHENOTYPE; SHEAR STRESS; STRESS STRAIN RELATIONSHIP; VISCOELASTICITY;

AEQUOREA VICTORIA; DICTYOSTELIUM; DICTYOSTELIUM DISCOIDEUM; VICTORIA;

EID: 0030069057     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(96)79651-0     Document Type: Article

References (41)

1. André, E., M. Brink, G. Gerisch, G. Isenberg, A. Noegel, M. Schleicher, J. E Segall, and E. Wallraff. 1989. A Dictyostelium mutant deficient in severin, an F-actin fragmenting protein, shows normal motility and chemotaxis. J. Cell Biol. 108:985-995.
2. Bray, D , and J Vasiliev. 1989. Networks from mutants. Nature. 338: 203-204.
3. Brink, M., G. Gerisch, G. Isenberg, A. A. Noegel, J. E. Segall, E. Wallraff, and M. Schleicher 1990. A Dictyostelium mutant lacking an F-actin cross-linking protein, the 120-kD gelation factor. J. Cell Biol. 111: 1477-1489.
4. Chalfie, M., Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher. 1994. Green fluorescent protein as a marker for gene expression. Science. 263:802-805.
5. Claviez, M., K. Pagh, H. Maruta, W. Baltes, P. Fisher, and G. Gerisch. 1982. Electron microscopic mapping of monoclonal antibodies on the tail region of Dictyostelium myosin. EMBO J. 1:1017-1022.
6. Condeelis, J. 1993. Life at the leading edge: the formation of cell protrusions. Annu. Rev Cell Biol. 9:411-444.
7. Cox, D., J. Condeelis, D. Wessels, D. Soll, H. Kern, and D A Knecht. 1992. Targeted disruption of the ABP120 gene leads to cells with altered motility. J. Cell Biol. 116.943-955.
8. Cox, D., J. A. Ridsdale, J. Condeelis, and J. Hartwig. 1995. Genetic deletion of ABP-120 alters the three-dimensional organization of actin filaments in Dictyostelium pseudopods. J. Cell Biol. 128:819-835.
9. Cunningham, C. C., J. B. Gorlin, D J. Kwiatkowski, J. H. Hartwig, P. A. Janmey, H. R. Byers, and T. P. Stossel. 1992. Actin-binding protein requirement for cortical stability and efficient locomotion Science. 255:325-327.
10. Elson, E. 1988. Cellular mechanics as an indicator of cytoskeletal structure and function. Annu. Rev. Biophys. Chem. 17:397-430.
11. Evans, E., and A. Yeung. 1989. Apparent viscosity and cortical tension of blood granulocytes determined by micropipet aspiration. Biophys. J. 56:151-160.
12. Grabski, S., X. G. Xie, J F. Holland, and M. Schindler. 1994. Lipids trigger changes in the elasticity of the cytoskeleton in plant cells: a cell optical displacement assay for live cell measurements. J. Cell Biol. 126:713-726.
13. Haugwitz, M., A. A. Noegel, J. Karakesisoglou, and M. Schleicher. 1994. Dictyostelium amoebae that lack G-actin-sequestering profilins show defects in F-actin content, cytokinesis, and development. Cell. 79: 303-314.
14. Hochmuth, R. M., and D. Needham. 1990. The viscosity of neutrophils and their transit times through small pores. Biorheology. 27:817-828.
15. Janmey, P. A. 1991. A torsion pendulum for measurement of the viscoelasticity of biopolymers and its application to actin networks. J. Biochem. Biophys. Methods. 22.41-53.
16. Janmey, P. A., S. Hvidt, J. Lamb, and T. P. Stossel. 1990. Resemblance of actin-binding protein/actin gels to covalently crosslinked networks. Nature. 345:89-92.
17. Leiting, B., and A. A. Noegel. 1991. The ble gene of Streptoalloteichus hindustanus as a new selectable marker for Dictyostelium discoideum confers resistance to phleomycin. Biochem. Biophys. Res. Commun. 180:1403-1407.
18. Manstein, D. J , M A. Titus, A. de Lozanne, and J. A. Spudich. 1989. Gene replacement in Dictyostelium: generation of myosin null mutants. EMBO J. 8:923-932.
19. Marsh, J. L., M. Erfle, and E. J. Wykes. 1984. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by inseruonal inactivation. Gene. 32:481-485.
20. Menkel, A., and H. Jockusch. 1991. Decreased osmotic stability of dystrophin-less muscle cells from the mdx mouse. Nature. 349:69-71.
21. Meyer, R. K., and U. Aebi. 1990. Bundling of actin filaments by α-actinin depends on its molecular length. J. Cell Biol. 110:2013-2024.
22. Noegel, A. A., B. Köppel, U. Gottwald, W. Witke, R. Albrecht, and M. Schleicher. 1995. Actin and actin-binding proteins in the motility of Dictyostelium. In The Cytoskeleton, 45: Colloquium-Mosbach 1994. Springer-Verlag, Berlin, 117-126.
23. Noegel, A. A., S. Rapp, F. Lottspeich, M. Schleicher, and M. Stewart. 1989. The Dictyostelium gelation factor shares a putative actin-binding site with α-actinins and dystrophin and also has a rod domain containing six 100-residue motifs that appear to have a cross-β conformation. J. Cell Biol. 109:607-618.
24. Noegel A. A., and W. Witke. 1988. Inactivation of the α-actinin gene in Dictyostelium Dev Genet. 9:531-538.
25. Noegel A., W. Witke, and M. Schleicher. 1987. Calcium-sensitive non-muscle α-actinin contains EF-hand structures and highly conserved regions. FEBS Lett. 221:391-396.
26. Pasternak, C., S. Wong, and E. L. Elson. 1995. Mechanical function of dystrophin in muscle cells. J. Cell Biol. 128:355-361.
27. Petersen, N. O., W. B. McConnaughey, and E. L. Elson. 1982. Dependence of locally measured cellular deformability on position on the cell, temperature, and cytochalasin B. Proc Natl. Acad. Sci. USA. 79: 5327-5331.
28. Sato, M., W. H. Schwarz, and T. D Pollard 1987. Dependence of the mechanical properties of actin/α-actinin gels on deformation rate. Nature. 325:828-830.
29. Schleicher, M., B. André, C. Andréoli, L Eichinger, M. Haugwitz, A. Hofmann, J. Karakesisoglou, M. Stöckelhuber, and A. A. Noegel. 1995. Structure/function studies on cytoskeletal proteins in Dictyostelium amoebae as a paradigm. FEBS Lett. 369:38-42.
30. Schleicher, M., and A. A. Noegel. 1992. Dynamics of the Dictyostelium cytoskeleton during chemotaxis. New Biol. 4:461-472.
31. Schleicher, M., A. Noegel, T. Schwarz, E. Wallraff, M. Brink, J. Faix, G. Gerisch, and G. Isenberg. 1988. A Dictyostelium mutant with severe defects in α-actinin: its characterization by cDNA probes and monoclonal antibodies. J. Cell Sci. 90:59-71.
32. Stossel, T. P 1993. On the crawling of animal cells. Science. 260: 1086-1094.
33. Wachsstock, D. H., W. H. Schwartz, and T. D. Pollard. 1993. Affinity of α-actinin for actin determines the structure and mechanical properties of actin filament gels. Biophys. J. 65:205-214.
34. Wallraff, E., M. Schleicher, M. Modersitzki, D. Rieger, G. Isenberg, and G. Gerisch. 1986. Selection of Dictyostelium mutants defective in cytoskeletal proteins: use of an antibody that binds to the ends of α-actinin rods. EMBO J. 5:61-67.
35. Wang, N., J. P. Butler, and D. E. Ingber. 1993. Mechanotransduction across the cell surface and through the cytoskeleton. Science. 260. 1124-1127.
36. Wilczynska, Z., and P. R. Fisher. 1994. Analysis of a complex plasmid insertion in a phototaxis-deficient transformant of Dictyostelium discotdeum selected on a Micrococcus luteus lawn. Plasmid. 32:182-194.
37. Witke, W., W. Nellen, and A. Noegel. 1987. Homologous recombination in the Dictyostelium α-actinin gene leads to an altered mRNA and lack of the protein. EMBO J. 6:4143-4148.
38. Witke, W., M. Schleicher, and A. A. Noegel. 1992. Redundancy in the microfilament system: abnormal development of Dictyostelium cells lacking two F-actin crosslinking proteins. Cell. 68:53-62.
39. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985 Improved M13 phage cloning vectors and host strains' nucleotide sequences of the M13, mp18. and pUC19 vectors. Gene. 33:103-119.
40. Zahalak, G. I. 1993. On the ambiguity of viscoelastic-fluid representations of cytoplasm subjected to large deformations. Biorheology. 30:471-472.
41. Zahalak, G I., W. B. McConnaughey, and E. L. Elson. 1990. Determination of cellular mechanical properties by cell poking, with an application to leukocytes. J. Biomech. Eng. 112:283-294.