Phenylalanine 30 plays an important role in receptor binding of verotoxin-1

Molecular Microbiology

Volumn 19, Issue 4, 1996, Pages 891-899

  Clark, Clifford ^a,f   Bast, Darrin ^a,b   Sharp, Allan M ^c   St Hilaire, Phaedria M ^d   Agha, Rummana ^a   Stein, Penelope E ^c   Toone, Eric J ^d   Read, Randy J ^c   Brunton, James L ^a,b,e  

^a MOUNT SINAI HOSPITAL   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c UNIVERSITY OF ALBERTA   (Canada)

^d DUKE UNIVERSITY   (United States)

^e TORONTO GENERAL HOSPITAL   (Canada)

^f LAB CENTRE FOR DISEASE CONTROL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

PHENYLALANINE; VEROTOXIN;

ANIMAL CELL; ARTICLE; BACTERIUM MUTANT; CONTROLLED STUDY; CYTOTOXICITY; ESCHERICHIA COLI; NONHUMAN; OPERON; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE; RECEPTOR BINDING; TOXICITY TESTING;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030068635     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1996.427962.x     Document Type: Article

References (45)

1. Armstrong, G.D., Fodor, E., and Vanmaele, R. (1991) Investigation of Shiga-like toxin binding to chemically synthesized oligosaccharide sequences. J Inf Dis 164: 1160-1167.
2. Basta, M., Karmali, M., and Lingwood, C. (1989) Sensitive receptor-specified enzyme-linked immunosorbent assay for Escherichia coli verocytotoxin. J Clin Microbiol 127: 1617-1622.
3. Bevington, P.R. (1969) Least-squares fit to an arbitrary function. In Data Reduction and Error Analysis for the Physical Sciences. New York: McGraw-Hill Book Company, pp. 235-246.
4. Bourne, Y., Rouget, P., and Cambillau, C. (1992) X-ray structure of a biantennary octasaccharide-lectinocomplex refined at 2.3 A resolution. J Biol Chem 267: 197-203.
5. Boyd, B., and Lingwood, C. (1989) Verotoxin receptor glycolipid in human renal tissue. Nephron 51: 207-210.
6. Boyd, B., Richardson, S., and Gariepy, J. (1991) Serological responses to the B subunit of Shiga-like toxin and its peptide fragments indicate that the B subunit is a vaccine candidate to counter the action of the toxin. Infect Immun 59: 750-757.
7. Brown, J.E., Echeverria, P., and Lindberg, A.A. (1991) Digalactosyl-containing glycolipids as cell surface receptors for Shiga toxin of Shigella dysenteriae 1 and related cytotoxins of Escherichia coli. Rev Inf Dis 13 (Suppl 4): S298-S303.
8. Brunton, J. (1994) Molecular biology and role in disease of the Verotoxins (Shiga-like toxins) of Escherichia coli. In Molecular Biology of Bacterial Pathogenesis, Miller, V., Kaper, J., Portnoy, D., and lsberg, R. (eds). Washington DC: ASM Press, pp. 391-404.
9. Cohen, A., Hannigan, G., Williams, B., and Lingwood, C. (1987) Roles of globotriosyl- and galabiosylceramide in verotoxin binding and high affinity interferon receptor. J Biol Chem 262: 17088-17099.
10. Cohen, A., Madrid-Marina, V., Estrov, Z., Freedman, M.H., Lingwood, C.A., and Dosch, H.M. (1990) Expression of glycolipid receptors to Shiga-like toxin on human B lymphocytes: a mechanism for the failure of long-lived antibody response to dysenteric disease. Int Immunol 2: 1-8.
11. Donohue-Rolfe, A., and Keusch, G.T. (1983) Shigella dysenteriae 1 cytotoxin: periplasmic protein releasable by polymyxin B and osmotic shock. Infect Immun 39: 270-274.
12. Dubois, M., Giles, K., Hamilton, J., Rebers, P., and Smith, F. (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350-356.
13. Edelhoch, H. (1967) Spectroscopic determination of tryptophan and tyrosine in proteins. Biochemistry 6: 1948-1954.
14. Fraser, M., Chernaia, M.M., Kozlov, Y.V., and James, M.N.G. (1994) Crystal structure of the holotoxin from Shigella dysenteriae at 2.5 Å resolution. Nature Struct Biol 1: 59-64.
15. Furutani, M., Kashigawa, K., Ito, K., Endo, Y., and Igarashi, K. (1992) Comparison of the modes of action of a Vero toxin (a Shiga-like toxin) from Escherichia coli, of ricin and of α-sarcin. Arch Biochem Biophys 293: 140-146.
16. Ghislain, J., Lingwood, C., and Fish, E. (1994) Evidence of glycosphingolipid modification of the Type I interferon receptor. J Immunol 153: 3655-3663.
17. Head, S.C., Karmali, M.A., and Lingwood, C.A. (1991) Preparation of VT1 and VT2 hybrid toxins from their purified dissociated subunits. Evidence for B subunit modulation of A subunit function. J Biol Chem 266: 3621-3627.
18. Jacewicz, M., Clausen, H., Nudelman, E., Donohue-Rolfe, A., and Kuesch, G.T. (1986) Pathogenesis of Shigella diarrhea. XI. Isolation of a Shigella toxin-binding glycolipid from rabbit jejunum and HeLa cells and its identification as globotriaosylceramide. J Exp Med 163: 1391-1404.
19. Jacewicz, M., Feldman, H.A., Donohue-Rolfe, A., Balasubramanian, A., and Keusch, G.T. (1989) Pathogenesis of Shigella diarrhea. XIV. Analysis of Shiga toxin receptors on cloned HeLa cells. J Inf Dis 159: 881-889.
20. Jacewicz, M., Mobassaleh, M., Gross, S., Balasubramanian, K., Daniel, P., Raghavan, S., McCluer, R., and Keusch, G.T. (1994) Pathogenesis of Shigella diarrhea: XVII. A mammalian cell membrane glycolipid Gb3 is required but not sufficient to confer sensitivity to Shiga toxin. J Inf Dis 169: 538-546.
21. Jackson, M.P., Wadolkowski, E.A., Weinstein, D.L., Holmes, R.K., and O'Brien, A.D. (1990) Functional analysis of the Shiga toxin and Shiga-like toxin type II variant binding subunits by using site-directed mutagenesis. J Bacteriol 172: 653-658.
22. Lindberg, A.A., Brown, J.E., Stromberg, N., Westling-Ryd, M., Schultz, J.E., and Karlsson, K.-A. (1987) Identification of the carbohydrate receptor for Shiga toxin produced by Shigella dysenteriae type I. J Biol Chem 262: 1779-1785.
23. Lingwood, C.A., and Yiu, S. (1992) Glycolipid modification of α-interferon binding: Sequence similarity between α-interferon receptor and the verotoxin (Shiga-like toxin) B subunit. Biochem J 283: 25-26.
24. Lingwood, C.A., Law, H., Richardson, S., Petric, M., Brunton, J.L., De Grandis, S., and Karmali, M. (1987) Glycolipid binding of purified recombinant Escherichia coli produced verotoxin in vitro. J Biol Chem 262: 8834-8839.
25. Maloney, M.D., and Lingwood, C.A. (1994) CD19 has a potential CD77 (globotriaosyl ceramide)-binding site with sequence similarity to verotoxin B-subunits: Implications of molecular mimicry for B cell adhesion and enterohemorrhagic Escherichia coli pathogenesis. J Exp Med 189: 191-201.
26. Nyholm, P.-G., and Pascher, I. (1993) Orientation of the saccharide chains of glycolipids at the membrane surface: conformational analysis of the glucose-ceramide and glucose-glyceride linkages using molecular mechanics (MM3). Biochemistry 32: 1225-1234.
27. Nyholm, P.-G., Brunton, J., and Lingwood, C. (1995) Modelling of the interaction of verotoxin-(VT1) with its glycolipid receptor globotriaosyl ceramide (Gb3). Int J Biol Macromol 17: 199-204.
28. Obrig, T., Louise, C., Lingwood, C., Boyd, B., Barley-Maloney, L., and Daniel, T. (1993) Endothelial heterogeneity in Shiga toxin receptors and responses. J Biol Chem 268: 15484-15488.
29. Perera, L.P., Samuel, J.E., Holmes, R.K., and O'Brien, A.D. (1991) Identification of three amino acid residues in the B subunit of Shiga toxin and Shiga-like toxin type II that are essential for holotoxin activity. J Bacteriol 173:1151-1160.
30. Petric, M., Karmali, M.A., Richardson, S.E., and Cheung, R. (1987) Purification and biological properties of Escherichia coli verocytotoxin. FEMS Microbiol Lett 41: 63-68.
31. Pudymaitis, A., Armstrong, G., and Lingwood, C.A. (1991) Verotoxin-resistant cell clones are deficient in the glycolipid globotriosylceramide: Differential basis of phenotype. Arch Biochem Biophys 286: 448-452.
32. Quiocho, F.A. (1986) Carbohydrate-binding proteins: tertiary structures and protein-sugar interactions. Annu Rev Biochem 55: 287-315.
33. Quiocho, F.A., Vyas, N.K., Sack, J.S., and Vyas, M.N. (1987) Atomic protein structures reveal basic features of binding of sugars and ionic substrates, and calcium cation. Cold Spring Harbor Symposia on Quantitative Biology LII: 453-463.
34. Ramotar, K., Boyd, B., Tyrrell, G., Gariepy, J., Lingwood, C., and Brunton, J. (1990) Characterization of Shiga-like toxin 1 B purified from overproducing clones of the SLT-1 B cistron. Biochem J 272: 805-811.
35. Sakabe, N. (1991) X-ray diffraction data collection system for modern protein crystallography with a Weissenberg camera and an imaging plate using synchrotron radiation. In Nuclear Instruments and Methods in Physical Research A303, pp. 448-463.
36. Schagger, H., and von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range of 1-100kDa. Anal Biochem 166: 368-379.
37. Sixma, T.K., Pronk, S.E., Kalk, K.H., vanZanten, B.A.M., Berghnis, A.M., and Hol, W.G.J. (1992) Lactose binding to heat-labile enterotoxin revealed by X-ray crystallography. Nature 355:561-564.
38. Sixma, T.K., Stein, P.E., Hol, W.G., and Read, R.J. (1993) Comparison of the B-pentamers of heat-labile enterotoxin and verotoxin-1: two structures with remarkable similarity and dissimilarity. Biochemistry 32: 191-198.
39. Stein, P.E., Boodhoo, A., Tyrrell, G.J., Brunton, J., and Read, R.J. (1992) Crystal structure of the cell-binding B oligomer of verotoxin-1 from E. coli. Nature 355: 748-750.
40. St. Hilaire, P., Boyd, M., and Toone, E. (1994) Interaction of the Shiga-like toxin 1 B-subunit with its carbohydrate receptor. Biochemistry 33: 14452-14463.
41. Strockbine, N., Marques, L., Holmes, R., and O'Brien, A. (1985) Characterization of monoclonal antibodies against Shiga-like toxin from Escherichia coli. Infect Immun 50: 695-700.
42. Stromberg, N., Nyholm, P.-G., Pascher, I., and Normark, S. (1991) Saccharide orientation at the cell surface affects glycolipid receptor function. Proc Natl Acad Sci USA 88: 9340-9344.
43. Tyrrell, G.J., Ramotar, K., Toye, B., Boyd, B., Lingwood, C.A., and Brunton, J. (1992) Alteration of the carbohydrate binding specificity of verotoxins from Galα1-4Gal to GalNacβ1-3 Gaα1-4Gal and vice versa by site-directed mutagenesis of the binding subunit. Proc Natl Acad Sci USA 89: 524-528.
44. Waddell, T., Cohen, A., and Lingwood, C.A. (1990) Induction of verotoxin sensitivity in receptor deficient cell lines using the receptor glycolipid globotriosyl ceramide. Proc Natl Acad Sci USA 87: 7898-7901.
45. Wiseman, T., Brandts, J., and Lin, L.-N. (1989) Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Anal Biochem 179: 131-137.