Synthesis of the β and β′ subunits of Escherichia coli RNA polymerase is autogenously regulated in vivo by both transcriptional and translational mechanisms

Molecular Microbiology

Volumn 19, Issue 3, 1996, Pages 483-493

  Dykxhoorn, Derek M ^a   St Pierre, Rebecca ^a   Linn, Thomas ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE;

ARTICLE; ENZYME SUBUNIT; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; PRIORITY JOURNAL;

DNA-DIRECTED RNA POLYMERASES; ENZYME REPRESSION; ESCHERICHIA COLI; FEEDBACK; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; LAC OPERON; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN BIOSYNTHESIS; RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; TRANSCRIPTION, GENETIC;

EID: 0030068536     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1996.384913.x     Document Type: Article

References (31)

1. Barry, G., Squires, C., and Squires, C.L. (1979) Control features within the rplJL-rpoBC transcription unit of Escherichia coli. Proc Natl Acad Sci USA 76: 4922-4926.
2. Bedwell, D.M., and Nomura, M. (1986) Feedback regulation of RNA polymerase subunit synthesis after the conditional overproduction of RNA polymerase in Escherichia coli. Mol Gen Genet 204: 17-23.
3. Clarke, D.J., and Maaloe, O. (1967) DNA replication and the division cycle in Escherichia coli. J Mol Biol 23: 99-112.
4. Dennis, P.P., Nene, V., and Glass, R.E. (1985) Autogenous post-transcriptional regulation of RNA polymerase β and β′ subunit synthesis in Escherichia coli. J Bacteriol 161: 803-806.
5. Engbaek, F., Gross, C., and Burgess, R.R. (1976) Quantitation of RNA polymerase subunits in Escherichia coli during exponential growth and after bacteriophage T4 infection. Mol Gen Genet 143: 291-295.
6. Fukuda, R., Taketo, M., and Ishihama, A. (1978) Autogenous regulation of RNA polymerase beta subunit synthesis in vitro. J Biol Chem 253: 4501-4504.
7. Guyer, M.S., Reed, R.R., Steitz, J.A., and Low, K.B. (1981) Identification of a sex-factor-affinity site in E. coli as γδ. Cold Spring Harbor Symp Quant Biol 45: 135-140.
8. BAD promoter. J Bacteriol 177: 4121-4130.
9. Hayward, R.S., and Fyfe, S.K. (1978) Over-synthesis and instability of sigma protein in a merodiploid strain of Escherichia coli. Mol Gen Genet 159: 89-99.
10. Iwakura, Y., Ito, K., and Ishihama, A. (1974) Biosynthesis of RNA polymerase in Escherichia coli. I. Control of RNA polymerase content at various growth rates. Mol Gen Genet 133: 1-23.
11. Kajitani, M., Fukuda, R., and Ishihama, A. (1980) Autogenous and post-transcriptional regulation of Escherichia coli RNA polymerase synthesis in vitro. Mol Gen Genet 179: 489-496.
12. Kirschbaum, J.B., and Konrad, E. (1973) Isolation of a specialized transducing bacteriophage carrying the β subunit gene for E. coli ribonucleic acid polymerase. J Bacteriol 116: 517-526.
13. Lang-Yang, H., and Zubay, G. (1981) Negative regulation of β and β′ synthesis by RNA polymerase. Mol Gen Genet 183: 514-517.
14. Lindahl, L., and Zengel, J.M. (1982) Expression of ribosomal genes in bacteria. Adv Genet 21: 53-121.
15. Linn, T., and Ralling, G. (1985) A versatile multiple- and single-copy vector system for the in vitro construction of transcriptional fusions to lacZ. Plasmid 14: 134-142.
16. Linn, T., and St. Pierre, R. (1990) Improved vector system for constructing transcriptional fusions that ensures independent translation of lacZ. J Bacteriol 172: 1077-1084.
17. Meek, D.W., and Hayward, R.S. (1986) Direct evidence for autogenous regulation of the Escherichia coli genes rpoBC in vivo. Mol Gen Genet 202: 500-508.
18. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, p. 352.
19. Moyle, H., Waldburger, C., and Susskind, M.M. (1991) Hierarchies of base pair preferences in the P22 ant promoter. J Bacteriol 173: 1944-1950.
20. Nomura, M., Gourse, R., and Baughman, G. (1984) Regulation of the synthesis of ribosomes and ribosomal components. Annu Rev Biochem 53: 75-117.
21. Passador, L., and Linn, T. (1989) Autogenous regulation of the RNA polymerase β subunit of Escherichia coli occurs at the translational level in vivo. J Bacteriol 171: 6234-6242.
22. Passador, L., and Linn, T. (1992) An internal region of rpoB is required for autogenous translational regulation of the β subunit of Escherichia coli RNA polymerase. J Bacteriol 174: 7174-7179.
23. Peacock, S., Cenetiempo, Y., Robakis, N., Brat, N., and Wiessback, H. (1982) In vitro synthesis of the first dipeptide of the β subunit of Escherichia coli RNA polymerase. Proc Natl Acad Sci USA 79: 4609-4612.
24. d18 and λdfus3. Mol Gen Genet 144: 339-343.
25. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
26. Scaife, J. (1976) Bacterial RNA polymerases: the genetics and control of their synthesis. In RNA Polymerase. Losick, R., and Chamberlin, M. (eds). Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, pp. 207-225.
27. St. Pierre, R., and Linn, T. (1996) A refined vector system for the in vitro construction of single-copy transcriptional or translational fusions to lacZ. Gene, in press.
28. Steward, K.L., and Linn, T. (1991) In vivo analysis of overlapping transcription units in the rplKAJLrpoBC ribosomal protein RNA polymerase gene cluster of Escherichia coli. J Mol Biol 218: 23-31.
29. Steward, K.L., and Linn, T. (1992) Transcription frequency modulates the efficiency of an attenuator preceding the rpoBC RNA polymerase genes of Escherichia coli: possible autogenous control. Nucl Acids Res 20: 4773-4779.
30. Yura, T., and Ishihama, A. (1979) Genetics of bacterial RNA polymerases. Annu Rev Genet 13: 59-97.
31. Zengel, J.M., and Lindahl, L. (1994) Diverse mechanisms for regulating ribosomal protein synthesis in Escherichia coli. Prog Nucl Acids Res Mol Biol 47: 331-370.