Mutational analysis of the RNase-like domain in subunit 2 of fission yeast RNA polymerase II

Molecular and General Genetics

Volumn 250, Issue 1, 1996, Pages 1-6

  Kawagishi Kobayashi, Makiko ^a,b   Yamamoto, Masayuki ^b   Ishihama, Akira ^a  

^a NATIONAL INSTITUTE OF GENETICS   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

Ribonuclease; RNA cleavage; RNA synthesis; Structure function relationships; Transcription

Indexed keywords

RIBONUCLEASE; RNA POLYMERASE II;

ARTICLE; GENETIC ANALYSIS; MUTATION; NONHUMAN; PRIORITY JOURNAL; RNA CLEAVAGE; RNA SYNTHESIS; SCHIZOSACCHAROMYCES POMBE;

EID: 0030068428     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02191819     Document Type: Article

References (20)

1. Azuma Y, Yamagishi M, Ishihama A (1991) Cloning and sequence determination of the Schizosaccharomyces pombe gene encoding the largest subunit of RNA polymerase II. Nucleic Acids Res 19: 461-468
2. Azuma Y, Yamagishi M, Ishihama A (1993) Subunits of the Schizosaccharomyces pombe RNA polymerase II: Enzyme purification and structure of the subunit 3. Nucleic Acids Res 21: 3749-3754
3. Borukhov S, Polyakov A, Nikiforov V, Goldfarb A (1993) GreA protein: a transcription elongation factor from Escherichia coli. Cell 72: 459-466
4. Egel R, Egel-Mitani M (1974) Premeiotic DNA synthesis in fission yeast. Exp Cell Res 88: 127-134
5. Gutz H, Heslot H, Leupold U, Loprieno N (1974) Schizosaccharomyces pombe. In: King RD (ed) Handbook of genetics, Plenum Press, New York-London, pp 395-445
6. Hill C, Dodson G, Heinemann U, Saenger W, Mitsui Y, Nakamura K, Borisov S, Tischenko G, Polykov K, Pavlovsky S (1983) The structural and sequence homology of a family of microbial ribonucleases. Trends Biochem Sci 8: 364-369
7. Ishihama A (1981) Subunit assembly of Escherichia coli RNA polymerase. Adv Biophys 14: 1-35
8. Izban MG, Luse DS (1992) The RNA polymerase II ternary complex cleaves the nascent transcript in a 3′-5′ direction in the presence of elongation factor SII. Genes Dev 6: 1342-1356
9. Kawagishi M, Yamagishi M, Ishihama A (1993) Cloning and sequence determination of the Schizosaccharomyces pombe rpb2 gene encoding the subunit 2 of RNA polymerase II. Nucleic Acids Res 21: 469-473
10. Kunkel TA (1985) Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci USA 82: 488-492
11. Meiering EM, Serrano L, Fersht AR (1992) Effect of active site residues in barnase on activity and stability. J Mol Biol 225: 585-589
12. Mossakowska DE, Nyberg K, Fersht AR (1989) Kinetic characterization of the recombinant ribonuclease from Bacillus amyloliquefaciens (Barnase) and investigation of key residues in catalysis by site-directed mutagenesis. Biochemistry 28: 3943-4850
13. Okazaki K, Okazaki N, Kume K, Jino S, Tanaka K, Okayama H (1990) High frequency transformation method and library-transducing vectors for cloning mammalian cDNAs by transcomplementation of Schizosaccharomyces pombe. Nucleic Acids Res 18: 6485-6489
14. Reines D (1992) Elongation factor-dependent transcript shortening by template-engaged RNA polymerase II. J Biol Chem 267: 3795-3800
15. Reines D, Mote Jr J (1993) Elongation factor SII-dependent transcription by RNA polymerase through a sequence-specific DNA-binding protein. Proc Natl Acad Sci USA 90: 1917-1921
16. Sherman F, Fink GR, Hicks JB (1986) Methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
17. Shirai T, Go M (1991) RNase-like domain in DNA-directed RNA polymerase II. Proc Natl Acad Sci USA 88: 9056-9060
18. Surratt CK, Milan SC, Chamberlin MJ (1991) Spontaneous cleavage of RNA in ternary complexes of Escherichia coli RNA polymerase and its significance for the mechanism of transcription. Proc Natl Acad Sci USA 88: 7983-7987
19. Sweetser D, Nonet M, Young RA (1987) Prokaryotic and eukaryotic RNA polymerases have homologous core subunits. Proc Natl Acad Sci USA 94: 1192-1196
20. Yura T, Ishihama A (1979) Genetics of bacterial RNA polymerases. Annu Rev Genet 13: 59-97