Dominant negative rat DNA polymerase β mutants interfere with base excision repair in Saccharomyces cerevisiae

Journal of Bacteriology

Volumn 178, Issue 3, 1996, Pages 656-661

  Clairmont, Caroline A ^a   Sweasy, Joann B ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DIRECTED DNA POLYMERASE BETA; MESYLIC ACID METHYL ESTER;

ARTICLE; ESCHERICHIA COLI; EXCISION REPAIR; MUTANT; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; ULTRAVIOLET RADIATION;

ESCHERICHIA COLI; EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 0030067386     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.3.656-661.1996     Document Type: Article

References (31)

1. Abbots, J., P. N. SenGupta, B. Zmuda, S. G. Widen, V. Notario, and S. H. Wilson. 1988. Expression of human DNA polymerase β in Escherichia coli and characterization of the recombinant enzyme. Biochemistry 27:901-909.
2. Blank, A., B. Kim, and L. Loeb. 1994. DNA polymerase δ is required for base excision repair of DNA methylation damage m Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 91:9047-9051.
3. Bork, P., C. Ouzounis, C. Sander, M. Scharf, R. Schneider, and E. Sonnhammer. 1992. Comprehensive sequence analysis of the 182 predicted open reading frames of yeast chromosome III. Protein Sci 1:1677-1690.
4. Casas-Finet, J. R., A. Kumar, R. L. Karpel, and S. H. Wilson. 1992. Mammalian DNA polymerase β. characterization of a 16-kDa transdomain fragment containing the nucleic acid-binding activities of the native enzyme Biochemistry 31:10272-10280.
5. Date, T., M. Yamaguchi, Y. Nishimoto, K. Tanihara, and A. Matsukage. 1988. Expression of active rat DNA polymerase β in Escherichia coli. Biochemistry 27:2983-2990.
6. Dresler, S. L., and M. W. Lieberman. 1983. Identification of DNA polymerases involved in DNA excision repair in diploid fibroblasts. J. Biol. Chem 258:9990-9994
7. Fornace, A. J., B. Zmudzka, M. C. Hollander, and S. H. Wilson. 1989. Induction of β-polymerase mRNA by DNA-damaging agents in Chinese hamster ovary cells. Mol. Cell. Biol. 9:851-853.
8. Friedberg, E. C. 1985. DNA repair W. H. Freeman and Co. New York.
9. Gu, H., J. D. Marth, P. C. Orban, H. Mossmann, and K. Rajewsky. 1994. Deletion of a DNA polymerase β gene segment in T cells using cell type-specific gene targeting. Science 265:103-106.
10. Hammond, R. A., J. K. McClung, and M. B. Miller. 1990. Effect of DNA inhibitors on DNA repair in intact and permeable human fibroblasts: evidence that DNA polymerase δ and β are involved in DNA repair synthesis induced by N-methyl-N′-nitro-N-nitrosoguanidine. Biochemistry 29:286-291.
11. Herskowitz, I. 1987. Functional inactivation of genes by dominant negative mutations. Nature (London) 329:219-222.
12. Husain, I., B. S. Morton, W. A. Beard, R. K. Singhal, R. Prasad, S. H. Wilson, and J. M. Besterman. 1995. Specific inhibition of DNA polymerase β by its 14kDa domain: role of single-and double-stranded DNA binding and 5′-phosphate recognition. Nucleic Acids Res. 23:1597-1603.
13. Kornberg, A., and T. A. Baker. 1992. DNA replication, 2nd ed. W. H. Freeman, New York.
14. Kupiec, M., and G. Simchen. 1986. DNA-repair characterization of cdc40-1, a cell-cycle mutant in Saccharomyces cerevisiae. Mutat. Res. 162:33-40.
15. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
16. Leem, S. H., P. A. Ropp, and A. Sugino. 1994. The yeast Saccharomyces cerevisiae DNA polymerase IV: possible involvement in double strand break DNA repair. Nucleic Acids Res. 22:3011-3017.
17. Miller, M. B., and L. H. Lui. 1982. Participation of different DNA polymerases in mammalian DNA repair synthesis is not related to patch size. Biochem. Biophys Res. Commun 108:1676-1682
18. Prasad, R., S. G. Widen, R. K. Singhal, J. Watkins, L. Prakash, and S. H. Wilson. 1993 Yeast open reading frame YCR14C encodes a DNA β-polymerase-like enzyme. Nucleic Acids Res 21:5301-5307.
19. Sancar, A., and G. B. Sancar. 1988. DNA repair enzymes. Annu. Rev. Biochem 57:29-67.
20. Sherman, F. 1991. Getting started with yeast, p. 3-21. In C Guthrie and G. R. Fink (ed.), Guide to yeast genetics and molecular biology. Academic Press, San Diego, Calif.
21. Shimizu, K., C. Santocanale, P. A. Ropp, M. P. Longhese, P. Plevani, G. Lucchini, and A. Sugino. 1993. Purification and characterization of a new DNA polymerase from budding yeast Saccharomyces cerevisiae: a probable homolog of mammalian DNA polymerase β. J. Biol. Chem. 268:27148-27153.
22. Singhal, R. K., R. Prasad, and S. H. Wilson. 1995. DNA polymerase β conducts the gap-filling step in uracil-initiaed base excision repair in bovine testis nuclear extract. J. Biol Chem. 270:949-957.
23. Singhal, R. K., and S. H. Wilson. 1993. Short gap-filling synthesis by DNA polymerase β is processive. J. Biol Chem. 268:15906-15911.
24. Sweasy, J. B. Unpublished data.
25. Sweasy, J. B., and L. A. Loeb. 1992. Mammalian DNA polymerase β can substitute for DNA polymerase I during DNA replication in Escherichia coli. J. Biol. Chem. 267:1407-1410.
26. Sweasy, J. B., and L. Loeb. 1994. Detection and characterization of mammalian DNA polymerase β mutants by functional complementation in Escherichia coli. Proc. Natl. Acad. Sci. USA 90:4626-4630.
27. Sweasy, J. B., and M. S. Yoon. 1995. Characterization of DNA polymerase β mutants with amino acid substitutions located in the C-terminal portion of the enzyme. Mol. Gen. Genet. 248:217-224.
28. Vogel, H. L., and D. M. Bonner. 1956. Acetylornithase of Escherichia coli partial purification and some properties. J. Biol. Chem. 218:97-106.
29. Wang, T. S., and D. Korn. 1982. Specificity of the catalytic interaction of human DNA polymerase β with nucleic acid substrates. Biochemistry 21: 1597-1608.
30. Wang, Z., X. Wu, and E. C. Friedberg. 1993. DNA repair synthesis during base excision repair in vitro is catalyzed by DNA polymerase ε and is influenced by DNA polymerases α and δ in Saccharomyces cerevisiae. Mol Cell. Biol. 13:1051-1058.
31. Witkin, E. M., and V. Roegnei-Maniscalco. 1992. Overproduction of DnaE protein (α subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I. J. Bacteriol. 174:4166-4168.