Improved hepatocyte culture system for studying the regulation of glycogen synthase and the effects of diabetes

Journal of Cellular Physiology

Volumn 166, Issue 1, 1996, Pages 208-216

  Van Auken, Moira ^a   Rulfs, Jill ^a,b   Buckholt, Michael A ^a   Garnache, Alice K ^a   Miller Jr , Thomas B ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^b Life Science and Bioengineering Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; GLYCOGEN SYNTHASE;

ALLOXAN DIABETES MELLITUS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; AUTORADIOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME REGULATION; GLYCOGEN METABOLISM; IMMUNOBLOTTING; LIVER CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RAT;

AGE FACTORS; ALLOXAN; ANIMALS; ANIMALS, NEWBORN; ANTIBODY SPECIFICITY; BLOTTING, WESTERN; CELL DIVISION; CELLS, CULTURED; CULTURE MEDIA; DIABETES MELLITUS, EXPERIMENTAL; DOSE-RESPONSE RELATIONSHIP, DRUG; GLUCOSE; GLYCOGEN SYNTHASE; LIVER; MITOGENS; RATS; RATS, SPRAGUE-DAWLEY; RIBONUCLEASES; RNA, MESSENGER;

ANIMALIA;

EID: 0030066494     PISSN: 00219541     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4652(199601)166:1<208::AID-JCP22>3.0.CO;2-9     Document Type: Article

References (30)

1. Antwi, D., Youn, J.H., Shargill, N.S., Lesikar, D.D., and Kaslow, H.R. (1988) Regulation of glycogen synthase in muscle and adipose tissue during fasting and refeeding. Am. J. Physiol., 254:E720-E725.
2. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K., eds. (1989) Current Protocols in Molecular Biology. Greene Publishing Associates and Wiley-Interscience, New York.
3. Bahnak, B.R., and Gold, A.H. (1982a) Isolation and characterization of liver glycogen synthase from diabetic rats. Arch. Biochem. Biophys., 213:492-503.
4. Bahnak, B.R., and Gold, A.H. (1982b) Effects of alloxan diabetes on the turnover of rat liver glycogen synthase: Comparison with liver phosphorylase. J. Biol. Chem., 257:8775-8780.
5. Bai, G., Zhang, Z., Werner, R., Nutall, F.Q., Tan, A.W.H., and Lee, E.C. (1990) The primary structure of rat liver glycogen synthase deduced by cDNA cloning. J. Biol. Chem., 265:7843-7848.
6. Bollen, M., Hue, L., and Stalmans, W. (1983) Effects of glucose on phosphorylase and glycogen synthase in hepatocytes from diabetic rats. Biochem. J., 210:783-787.
7. Cuidad, C.J., Carabaza, A., Bosch, F., and Foix, A.M.G. (1988) Glycogen synthase activation by sugars in isolated hepatocytes. Arch. Biochem. Biophys., 264.30-39.
8. Fernandez-Novell, J.M., Arino, J., Vilaro, S., and Guinovart, J.J. (1992a) Glucose induces the translocation and the aggregation of glycogen synthase in rat hepatocytes. Biochem. J., 281:443-448.
9. Fernandez-Novell, J.M., Arino, J , Vilaro, S., Bellido, D., and Guinovart, J.J. (1992b) Role of glucose 6-phosphate in the translocation of glycogen synthase in rat hepatocytes. Biochem. J., 288:497-501.
10. Hsu, S.D., Jaspers, S.R., Davis, B.B., Cardell, R.R., Miller, T.B., Jr., and Drake, R.L. (1990) Appearance of a nonfunctional isozyme of hepatic glycogen synthase in late gestation. Arch. Biochem. Biophys., 281:152-156.
11. Kaslow, H.R., Lesikar, D.D., Antivi, D., and Tan, A.W.H. (1985) L-type glycogen synthase: Tissue distribution and electrophoretic mobility. J. Biol. Chem., 260.9953-9956.
12. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227:680-685.
13. Lavoie, L., Bollen, M., Stalmans, W., and Van de Werve, G. (1991) Increased synthase phosphatase activity is responsible for the super-activation of glycogen synthase in hepatocytes from fasted obese Zucker rats. Endocrinology, 129:2674-2678.
14. Lima, F., Bao, S., and Garvey, T. (1994) Biological actions of insulin are differentially regulated by glucose and insulin in primary culture hepatocytes. Diabetes, 43:53-62.
15. Miller, T.B., Jr. (1978) Effects of diabetes on glucose regulation of enzymes involved in hepatic glycogen metabolism. Am. J. Physiol., 234:E13-E19.
16. Miller, T B., Jr. (1979) Glucose activation of liver glycogen synthase: Insulin mediated restoration of glucose effect in diabetic rats is blocked by protein synthesis inhibitor. Biochim. Biophys. Acta., 583:36-46.
17. Miller, T.B., Jr., Vicalvi, J.J., Jr., and Garnache, A.K. (1981) Alteration of hepatic glycogen synthase phosphatase activity by insulin action. Am. J. Physiol., 240:E539-E543.
18. Miller, T.B., Jr., Garnache, A.K., and Cruz, J. (1984) Insulin regulation of glycogen synthase phosphatase in primary cultures of rat hepatocytes. J. Biol. Chem., 259:12470-12474.
19. Miller, T.B., Jr., Garnache, A.K., Cruz, J., McPherson, R.K., and Wolleben, C.D. (1986) Regulation of glycogen metabolism in primary cultures of rat hepatocytes. J. Biol. Chem., 261:785-790.
20. Moses, R.E., and Hurt, M.M. (1983) Replication and repair of DNA. In: Laboratory Methods Manual for Hormone Action and Molecular Endocrinology. W.T. Shrader and B.W. O'Malley, eds. Houston Biological Association, Houston, TX, 7th ed., p. 7.
21. Nuttall, F.Q., Gilboe, D.P., Gannon, M.C., Niewoehner, C.B., and Tan, A.W.H. (1988) Regulation of glycogen synthesis in the liver. Am. J. Med., 85:77-85.
22. Roach, P.J. (1990) Control of glycogen synthase by hierarchical protein phosphorylation. FASEB J., 4:2961-2968.
23. Rodriguez-Gil, J.E., Guinovart, J.J., and Bosch, F. (1993) Lithium restores glycogen synthesis from glucose in hepatocytes from diabetic rats. Arch. Biochem. Biophys., 301:411-415.
24. Rulfs, J., Jaspers, S.R., Garnache, A.K., and Miller, T.B., Jr. (1989) Phosphorylase synthesis in diabetic hepatocytes and cardiomyocytes. Am. J. Physiol., 257:E74-E80.
25. Seglen, P.O. (1973) Preparation of rat liver cells. Exp. Cell Res., 82:391-398.
26. 32P] phosphates. J. Biol. Chem., 260:4751-4757.
27. 32P] phosphate. Biochem. Cell Biol., 71:90-96.
28. 14C-glucose. Anal. Biochem , 25:486-499.
29. Wititsuwannakul, D., and Kim, K. (1979) Immunologic studies of liver glycogen synthase: Relative significance of covalent modification and changes in the rate of synthesis and degradation. J. Biol. Chem , 254:3562-3569.
30. Zheng, Q., Levitsky, L.L., Fan, J., Ciletti, N., and Mink, N. (1992) Glycogenesis in the cultured fetal and adult rat hepatocytes is differently regulated by medium glucose. Pediatr. Res., 32:714-718.