What is the relationship between the metabolism of preimplantation embryos and their developmental competence?

Molecular Reproduction and Development

Volumn 43, Issue 1, 1996, Pages 105-133

  Barnett, Deborah K ^a   Bavister, Barry D ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Energy production; In vitro development; Mammalian embryo; Preimplantation

Indexed keywords

ANIMAL TISSUE; CELL VIABILITY; CITRIC ACID CYCLE; EMBRYO; ENERGY TRANSFER; FETUS DEVELOPMENT; MOUSE; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PREIMPLANTATION EMBRYO; PRIORITY JOURNAL; REVIEW; TISSUE CULTURE;

AMINO ACIDS; ANIMALS; BLASTOCYST; EMBRYONIC AND FETAL DEVELOPMENT; ENERGY METABOLISM; FATTY ACIDS; FEMALE; GLUCOSE; GLYCOLYSIS; HUMANS; LUMINESCENT MEASUREMENTS; MAMMALS; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; MODELS, BIOLOGICAL; ORGAN CULTURE TECHNIQUES; OXYGEN CONSUMPTION;

ANIMALIA; MAMMALIA;

EID: 0030065590     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2795(199601)43:1<105::AID-MRD13>3.0.CO;2-4     Document Type: Review

References (277)

1. Abramczuk J, Solter D, Koprowski H (1977): The beneficial effect of EDTA on development of mouse one-cell embryos in chemically defined medium. Dev Biol 61:378-383.
2. Adams CE, Moor RM, Rowson LEA (1968): Survival of cow and sheep eggs in the rabbit oviduct. Proc VIth Int Congr Anim Reprod AI 1.573-574.
3. Barbehenn EK, Wales RG, Lowry OH (1974): The explanation for the blockade of glycolysis in early mouse embryos. Proc Natl Acad Sci USA 71:1056-1060.
4. Barbehenn EK, Wales RG, Lowry OH (1978): Measurement of metabolites in single preimplantation embryos. A new means to study metabolic control in early embryos. J Embryol Exp Morphol 43: 29-46.
5. Barnett DK (1993): Sensitivity of hamster 2-cell embryos to glucose and phosphate is restricted to a brief period immediately before cleavage. Biol Reprod 48(Suppl 1):176.
6. Barnett DK, Bavister BD (1992): Hypotaurine requirement for in vitro development of golden hamster one-cell embryos into morulae and blastocysts, and production of term offspring from in vitrofertilized ova. Biol Reprod 47:297-304.
7. Barnett DK, Bavister BD (1996): Inhibitory effect of glucose and phosphate on the second cleavage division of hamster embryos: Is it linked to metabolism? Human Reprod (in press).
8. Barnett DK, Kimura J, Bavister BD (1995): Quantitative analysis of distribution of active mitochondria in developing and non-developing hamster 2-cell embryos. Biol Reprod 52(Suppl 1):180
9. Barnett DK, Kimura J, Bavister BD (1996): Translocation of active mitochondria during hamster preimplantation embryo development studied by confocal laser scanning microscopy. Dev Dynamics (in press).
10. Barnett DK, Rieger D, Bavister BD (1993): Changes in the metabolism of glucose and glutamine during development of the hamster embryo. Theriogenology 39:185.
11. Batt PA, Gardner DK, Cameron AWN (1991): Oxygen concentration and protein source affect the development of preimplantation goat embryos in vitro. Reprod Fertil Dev 3:601-607.
12. Bavister BD (1987): Studies on the developmental blocks in cultured hamster embryos. In BD Bavister (ed): "The Mammalian Preimplantation Embryo." New York: Plenum Press, pp 219-249.
13. Bavister BD (1988): Role of oviductal secretions in embryonic growth in vivo and in vitro. Theriogenology 29:143-154.
14. Bavister BD (1992): Co-culture for embryo development: Is it really necessary? Hum Reprod 7:1339-1341.
15. Bavister BD (1995): Culture of preimplantation embryos: Facts and artifacts. Hum Reprod Update 1:91-148.
16. Bavister BD, McKiernan SH (1993): Regulation of hamster embryo development in vitro by amino acids. In BD Bavister (ed): "Preimplantation Embryo Development." New York: Springer-Verlag, pp 57-72.
17. Bavister BD, Leibfried ML, Lieberman G (1983): Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol Reprod 28:235-247.
18. Benos DJ, Balaban RS (1980): Energy requirements of the developing mammalian blastocyst for active ion transport. Biol Reprod 23:941-947.
19. Better-bed B, Wright RW (1985): Development of one-cell ovine embryos in two culture media under two gas atmospheres. Theriogenology 23:547-553.
20. Biggers JD (1987): Pioneering mammalian embryo culture. In BD Bavister (ed): "The Mammalian Preimplantation Embryo. Regulation of Growth and Differentiation in Vitro." New York: Plenum Press, pp 1-22.
21. Biggers JD, Whittingham DG, Donahue RP (1967): The pattern of energy metabolism in the mouse oocyte and zygote. Proc Natl Acad Sci USA 58:560-567.
22. Biggers JD, Lawitts JA, Lechene CP (1993): The protective action of betaine on the deleterious effects of NaCl on preimplantation mouse embryos in vitro. Mol Reprod Dev 34:380-390.
23. Boatman DE, Robbing RS (1991): Bicarbonate: Carbon-dioxide regulation of sperm capacitation, hyperactivated motility, and acrosome reactions. Biol Reprod 44:806-813.
24. Brackett BG, Mastroianni L (1974): Composition of oviductal fluid. In AD Johnson, CW Foley (eds): "The Oviduct and Its Functions." New York: Academic Press Inc., pp 133-159.
25. Brinster RL (1965a): Studies on the development of mouse embryos in vitro. IV. Interaction of energy sources. J Reprod Fertil 10:227-240.
26. Brinster RL (1965b): Studies on the development of mouse embryos in vitro II. The effect of energy source. J Exp Zool 158:59-68.
27. Brinster RL (1965c): Studies on the development of mouse embryos in vitro HI. The effect of fixed nitrogen source. J Exp Zool 158:69-78.
28. Brinster RL (1967): Carbon dioxide production from glucose by the preimplantation mouse embryo. Exp Cell Res 47:271-277.
29. Brinster RL (1968a): Carbon dioxide production from glucose by the preimplantation rabbit embryo. Exp Cell Res 51:330-334.
30. Brinster RL (1968b): In vitro culture of mammalian embryos. J Anim Sci 27:1-14.
31. Brinster RL (1971): Mammalian embryo metabolism. In RJ Blandau (ed): "The Biology of the Blastocyst." Chicago: University of Chicago Press, pp 303-317.
32. Brinster RL (1972): Cultivation of the mammalian egg. In G Rothblat, V Cristofalo (eds): "Growth, Nutrition and Metabolism of Cells in Culture," Vol II. New York: Academic Press, pp 251-286.
33. Brinster RL, Thomson JL (1966): Development of eight-cell mouse embryos in vitro. Exp Cell Res 42 308-315.
34. Bnson DR, Leese HJ (1991): Energy metabolism in late preimplantation rat embryos. J Reprod Fertil 93:245-251.
35. Brison DR, Leese HJ (1994): Blastocoel cavity formation by preimplantation rat embryos in the presence of cyanide and other inhibitors of oxidative phosphorylation. J Reprod Fertil 101:305-309.
36. Brison DR, Hewitson LC, Leese HJ (1993): Glucose, pyruvate, and lactate concentrations in the blastocoel cavity of rat and mouse embryos. Mol Reprod Dev 35:227-232.
37. Brown JJG, Whittingham DG (1991): The roles of pyruvate, lactate and glucose during preimplantation development of embryos from F1 hybrid mice in vitro. Development 112:99-105.
38. Brown JJG, Whittingham DG (1992): The dynamic provision of different energy substrates improves development of one-cell randombred mouse embryos in vitro. J Reprod Fertil 95:503-511.
39. Busa WB, Nuccitelli R (1984): Metabolic regulation via intracellular pH. Am J Physiol 246:R409-438.
40. Butler JE, Biggers JD (1989): Assessing the viability of preimplantation embryos in vitro (livestock). Theriogenology 31:115-126.
41. Butler JE, Williams JE (1990): Preliminary characterization of pyruvate uptake by one-cell ovine embryos. Theriogenology 33:423-432.
42. Butler JE, Lechene C, Biggers JD (1988): Noninvasive measurement of glucose uptake by two populations of murine embryos. Biol Reprod 39:779-786.
43. Carney EW, Bavister BD (1987a): Stimulatory and inhibitory effects of amino acids on the development of hamster eight-cell embryos in vitro. J In Vitro Fertil Embryo Transfer 4:162-167.
44. Carney EW, Bavister BD (1987b): Regulation of hamster embryo development in vitro by carbon dioxide. Biol Reprod 36:1155-1163.
45. Chatot CL, Ziomek CA, Bavister BD, Lewis JL, Torres I (1989): An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. J Reprod Fertil 86:679-688.
46. Chatot CL, Lewis JL, Torres I, Ziomek CA (1990a): Development of 1-cell embryos from different strains of mice in CZB medium. Biol Reprod 42:432-440.
47. Chatot CL, Tasca RJ, Ziomek CA (1990b): Glutamine uptake and utilization by preimplantation mouse embryos in CZB medium. J Reprod Fertil 89:335-346.
48. Chatot CL, Lewis-Williams J, Torres I. Ziomek CA (1994): One-minute exposure of 4-cell mouse embryos to glucose overcomes morula block in CZB medium. Mol Reprod Dev 37:407-412.
49. Cholewa JA, Whitten WK (1970): Development of two-cell mouse embryos in the absence of a fixed-nitrogen source. J Reprod Fertil 22:553-555.
50. Chung BH, Chung HM, Uhm SJ, Chang WK, Seung KR, Lee HT (1994): Organ culture of in vitro produced porcine embryos from follicular oocytes using mouse oviduct. Theriogenology 41:178.
51. Clarke RN, Baltz JM, Lechene CP, Biggers JD (1989): Use of ultramicrofluorometric methods for the study of single preimplantation embryos. Poultry Sci 68:972-978.
52. Conaghan J, Handyside AH, Winston RML, Leese HJ (1993a): Effects of pyruvate and glucose on the development of human preimplantation embryos in vitro. J Reprod Fertil 99:87-95.
53. Conaghan J, Hardy K, Handyside AH, Winston RML, Leese HJ (1993b): Selection criteria for human embryo transfer-A comparison of pyruvate uptake and morphology. J Assisted Reprod Genet 10:21-30.
54. Davis DL, Day BN (1978): Cleavage and blastocyst formation by pig eggs in vitro. J Anim Sci 46:1043-1053.
55. de Hemptinne A, Marrannes R, Vanheel B (1983): Influence of organic acids on intracellular pH. Am J Physiol 245:178-183.
56. De Hertogh R, Vanderheyden I, Pampfer S, Robin D, Dufrasne E, Delcourt J (1991): Stimulatory and inhibitory effects of glucose and insulin on rat blastocyst development in vitro. Diabetes 40:641-647.
57. Denk W, Strickler JH, Webb WW (1990): Two-photon laser scanning fluorescence microscopy. Science 248:73-76.
58. Dorland M, Gardner DK, Trounson AO (1994): Serum in synthetic oviduct fluid causes mitochondrial degeneration in ovine embryos. J Reprod Fertil Abstr Ser 13:25 (abstract no. 70)
59. Du ZF, Wales RG (1993a): Glycolysis and glucose oxidation by the sheep conceptus at different oxygen concentrations. Reprod Fertil Dev 5:383-393.
60. Du ZF, Wales RG (1993b): Effect of culture from the zygote stage on the metabolism of glucose and glutamine by 2-cell embryos and blastocysts recovered from outbred or F1 hybrid female mice. Reprod Fertil Dev 5:555-565.
61. Du ZF, Wales RG (1993c): Some effects of genotype and composition of the culture medium on the development of mouse zygotes in vitro. Reprod Fertil Dev 5:405-415.
62. Dufrasnes E, Vanderheyden I, Robin D, Delcourt J, Pampfer S, De Hertogh R (1993): Glucose and pyruvate metabolism in preimplantation blastocysts from normal and diabetic rats. J Reprod Fertil 98:169-177.
63. Dumoulin JCM, Evers JLH, Bras M, Pieters MHEC, Geraedts JPM (1992): Positive effect of taurine on preimplantation development of mouse embryos in vitro. J Reprod Fertil 94:373-380.
64. Dunglison GF, Kaye PL (1993): Insulin regulates protein metabolism in mouse blastocysts. Mol Reprod Dev 36:42-48.
65. Edirisinghe WR, Wales RG (1985): Influence of environmental factors on the metabolism of glucose by preimplantation mouse embryos in vitro. Aust J Biol Sci 38:411-420.
66. 14C]glucose during culture of 8-cell and morula - Early blastocyst-stage mouse embryos in vitro and in vivo. J Reprod Fertil 72:59-65.
67. Ellington JE, Carney EW, Farrell PB, Simkin ME, Foote RH (1990): Bovine 1-2 cell embryo development using a simple medium in three oviduct epithelial cell coculture systems. Biol Reprod 43:97-104.
68. Engel W, Petzoldt U (1973): Early developmental changes of the lactate dehydrogenase isoenzyme pattern in mouse, rat, guinea-pig, Syrian hamster and rabbit. Humangenetik 20:125-131.
69. Erbach GT, Lawitts JA, Papaioannou VE, Biggers JD (1994): Differential growth of the mouse preimplantation embryo in chemically defined media. Biol Reprod 50:1027-1033.
70. Ericsson SA, Garner DL, Redelman D, Ahmad K (1989): Assessment of the viability and fertilizing potential of cryopreserved bovine spermatozoa using dual fluorescent staining and two-flow cytometric systems. Gamete Res 22:355-368.
71. Eyestone WH, Leibfried-Rutledge ML, Northey DL, Gilligan BG, First NL (1987): Culture of one- and two-cell bovine embryos to the blastocyst stage in the ovine oviduct. Theriogenology 28:1-7.
72. Fahy MM, Kane MT (1992): Inositol stimulates DNA and protein synthesis, and expansion by rabbit blastocysts in vitro. Hum Reprod 7:550-552.
73. Farrell PS, Bavister BD (1984): Short-term exposure of two-cell hamster embryos to collection media is detrimental to viability. Biol Reprod 31:109-114.
74. Farrell PB, Foote RH (1995): Beneficial effects of culturing rabbit zygotes to blastocysts in 5% oxygen and 10% carbon dioxide. J Reprod Fertil 103:127-130.
75. Fischer B, Bavister BD (1993): Oxygen tension in the oviduct and uterus of rhesus monkeys, hamsters and rabbits. J Reprod Fertil 99:673-679.
76. Fissore RA, Jackson KV, Kiessling AA (1989): Mouse zygote development in culture medium without protein in the presence of ethylenediaminetetraacetic acid. Biol Reprod 41:835-841.
77. Flood MR, Wiebold JL (1988): Glucose metabolism by preimplantation pig embryos. J Reprod Fertil 84:7-12.
78. 14C]glucose in preimplantation mouse embryos in culture. Biol Reprod 19:922-926.
79. Flynn TJ, Hillman N (1980): The metabolism of exogenous fatty acids by preimplantation mouse embryos developing in vitro. J Embryol Exp Morphol 56:157-168.
80. Fridhandler L (1961): Pathways of glucose metabolism in fertilized rabbit ova at various preimplantation stages. Exp Cell Res 22:303-316.
81. Gardner DK (1994): Mammalian embryo culture in the absence of serum or somatic cell support. Cell Biol Int 18:67.
82. Gardner DK, Lane M (1993a): Amino acids and ammonium regulate mouse embryo development in culture. Biol Reprod 48:377-385.
83. Gardner DK, Lane M (1993b): The 2-cell block in CF1 mouse embryos is associated with an increase in glycolysss and a decrease in tricarboxylic acid cycle activity: Alleviation of the 2-cell block is associated with the restoration of in vivo metabolic pathway activities. Biol Reprod 48 (Suppl 1):96.
84. Gardner DK, Leese HJ (1986): Non-invasive measurement of nutrient uptake by single cultured preimplantation mouse embryos. Hum Reprod 1:25-27.
85. Gardner DK, Leese HJ (1987): Assessment of embryo viability prior to transfer by the noninvasive measurement of glucose uptake. J Exp Zool 42:103-105.
86. Gardner DK, Leese HJ (1988): The role of glucose and pyruvate transport in regulating nutrient utilization by preimplantation mouse embryos. Development 104:423-429.
87. Gardner DK, Leese HJ (1990). Concentrations of nutrients in mouse oviduct fluid and their effects on embryo development and metabolism in vitro J Reprod Fertil 88:361-368.
88. Gardner DK, Leese HJ (1993): Assessment of embryo metabolism and viability. In A Trounson, DK Gardner (eds): "Handbook of In Vitro Fertilization." Boca Raton, FL: CRC Press Inc, pp 195-212.
89. Gardner DK, Sakkas D (1993): Mouse embryo cleavage, metabolism and viability: Role of medium composition. Hum Reprod 8:288-295.
90. Gardner DK, Clarke RN, Lechene CP, Biggers JD (1989): Development of a noninvasive ultramicrofluorometric method for measuring net uptake of glutamine by single preimplantation mouse embryos. Gamete Res 24:427-438.
91. Gardner DK, Lane M, Batt P (1993): Uptake and metabolism of pyruvate and glucose by individual sheep preattachment embryos developed in vivo. Mol Reprod Dev 36:313-319.
92. Gardner DK, Lane M, Spitzer A, Batt PA (1994a): Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: Amino acids, vitamins, and culturing embryos in groups stimulate development Biol Reprod 50:390-400.
93. Gardner DK, Lane M, Calderon I, Leeton J (1994b): Metabolic concentrations in human oviduct and uterine fluids throughout the menstrual cycle. Proceedings of the 50th Annual Meeting American Fertility Soc S79 (abs. O-161).
94. Ginsberg L, Hillman N (1973): ATP metabolism in cleavage-stage mouse embryos. J Embryol Exp Morphol 30:267-282.
95. Gott AL, Hardy K, Winston RML, Leese HJ (1990): Non-invasive measurement of pyruvate and glucose uptake and lactate production by single human preimplantation embryos. Hum Reprod 5:104-108.
96. Grandin N, Charbonneau M (1991): Cycling of intracellular free calcium and intracellular pH in Xenopus embryos: A possible role in the control of the cell cycle. J Cell Sci 99:5-11.
97. Grant P, Nilsson BO, Bergstrom S (1977): The ultrastructure of preimplantation hamster blastocysts developed in vivo and in vitro. Fertil Steril 28:866-870.
98. Gray CW, Morgan PM, Kane MT (1992): Purification of an embryotrophic factor from commercial bovine serum albumin and its identification as citrate. J Reprod Fertil 94:471-480.
99. Hall JL, Stephenson RB, Mathias CA, Brackett BG (1977): Hormonal dependence of cyclic patterns in hamster uterine fluid proteins. Biol Reprod 17:738-744.
100. Ham RG (1963): An improved nutrient solution for diploid Chinese hamster and human cell lines. Exp Cell Res 29:515-526.
101. Hammond J Jr (1949): Recovery and culture of tubal mouse ova. Nature 163:28-29.
102. Hamner CE, Fox SB (1969): Biochemistry of oviductal secretions. In ESE Hafez, RJ Blandau (eds): "The Mammalian Oviduct." Chicago: University of Chicago Press, pp 333-355.
103. Hardy K, Hooper MAK, Handyside AH, Rutherford AJ, Winston RML, Leese HJ (1989): Non-invasive measurement of glucose and pyruvate uptake by individual human oocytes and preimplantation embryos. Hum Reprod 4:188-191.
104. Hewitson LC, Leese HJ (1993): Effects of metabolic inhibitors on cell number, development and metabolism of isolated mouse inner cell masses Biol Reprod 48(Suppl 1):174 (abstract 463).
105. Hillman N, Tasca RJ (1969): Ultrastructural and autoradiographic studies of mouse cleavage stages. Am J Anat 126:151-174.
106. Ho Y, Doherty AS, Schultz RM (1994): Mouse preimplantation embryo development in vitro: Effect of sodium concentration in culture media on RNA synthesis and accumulation and gene expression. Mol Reprod Dev 38:131-141.
107. Hooper MAK, Leese HJ (1989): Activity of hexokinase in mouse oocytes and preimplantation embryos. Biochem Soc Trans 17:546-547.
108. Hunter GL, Bishop GP, Adams CE, Rowson LEA (1962): Successful long-distance aerial transport of fertilized sheep ova. J Reprod Fertil 3:33-40.
109. Hunter DR, Haworth RA, Southard JH (1976): Relationship between configuration, function, and permeability in calcium-treated mitochondria. J Biol Chem 251:5069-5077.
110. Javed MH, Wright RW Jr (1991): Determination of pentose phosphate and Embden-Meyerhof pathway activities in bovine embryos. Theriogenology 35:1029-1037.
111. Johnson MH, Nasr-Esfahani MH (1994): Radical solutions and cultural problems: Could free oxygen radicals be responsible for the impaired development of preimplantation mammalian embryos in vitro? Bioassays 16:31-38.
112. Kane MT (1979): Fatty acids as energy sources for culture of one-cell rabbit ova to viable morulae. Biol Reprod 20:323-332.
113. Kane MT (1983): Variability in different lots of commercial bovine serum albumin affects cell multiplication and hatching of rabbit blastocysts in culture. J Reprod Fertil 69:555-558.
114. Kane MT (1987a): In vitro growth of preimplantation rabbit embryos. In BD Bavister (ed): "The Mammalian Preimplantation Embryo." New York: Plenum Press, pp 193-217
115. Kane MT (1987b): Minimal nutrient requirements for culture of one-cell rabbit embryos. Biol Reprod 37:775-778.
116. Kane MT (1988): The effects of water soluble vitamins on the expansion of rabbit blastocysts in vitro. J Exp Zool 245:220-223.
117. Kane MT, Bavister BD (1988): Vitamin requirements for development of eight-cell hamster embryos to hatching blastocysts in vitro. Biol Reprod 39:1137-1143.
118. Kane MT, Buckley NJ (1977): The effects of inhibitors of energy metabolism on the growth of one-cell rabbit ova to blastocysts in vitro. J Reprod Fertil 49:261-266.
119. Kane MT, Foote RH (1970): Culture of two- and four-cell rabbit embryos to the expanding blastocyst stage in synthetic media. Proc Soc Exp Biol Med 133:921-925.
120. + exchange in verte-brate rod photoreceptors. Exp Eye Res 51:199-207.
121. Katz J, Landau BR, Bartsch GE (1966): The pentose cycle, triose phosphate isomerization, and lipogenesis in rat adipose tissue. J Biol Chem 241:727-740.
122. Kaye PL (1986): Metabolic aspects of the physiology of the preimplantation embryo. In J Rossant, RA Pedersen (eds): "Experimental Approaches to Mammalian Embryonic Development." Cambridge. UK: Cambridge University Press, pp 267-292.
123. Khurana NK (1992): Comparative aspects of the energy metabolism of bovine embryos from the oocyte to the hatched blastocyst derived in vitro and in vivo. Dissertation for DMV, Hochschule Hannover.
124. 14C]glucose by mouse morulae-early blastocysts in vitro. J Reprod Pertil 79:275-280.
125. 14]glucose by mouse morulae and early blastocysts in vitro. J Reprod Fertil 79:267-273.
126. Khurana NK, Wales RG (1987c): Effects of coculture with uterine epithelial cells on the metabolism of glucose by mouse morulae and early blastocysts. Aust J Biol Sci 40:389-395.
127. 14C]glucose by mouse morulae and early blastocysts in vitro. Reprod Fertil Dev 1:99-106.
128. 14]glucose by mouse morulae and early blastocysts in vitro. Reprod Fertil Dev 1:89-98.
129. Kim JH, Funahashi H, Niwa K, Okuda K (1993a): Glucose requirement at different developmental stages of in vitro fertilized bovine embryos cultured in semi-defined medium. Theriogenology 39:875-886.
130. Kim JH, Niwa K, Lim JM, Okuda K (1993b): Effects of phosphate, energy substrates, and amino acids on development of in vitro-matured, in vitro-fertilized bovine oocytes in a chemically defined, protein-free culture medium. Biol Reprod 48:1320-1325.
131. Kimura J, Barnett DK, Bavister BD (1994): Organization of active mitochondria in hamster preimplantation embryos analyzed by confocal laser scanning microscopy. Biol Reprod 50 (Suppl 1):70 (abstract 641.
132. Kishi J, Noda Y, Narimoto K, Umaoka Y, Mori T (1991): Block to development in cultured rat 1-cell embryos is overcome using medium HECM-1. Hum Reprod 6:1445-1448.
133. Koobs DH (1972): Phosphate mediation of the Crabtree and Pasteur effects. Science 178:27-133.
134. Lamb VK, Leese HJ (1994): Uptake of a mixture of amino acids by mouse blastocysts. J Reprod Fertil 102: 169-175.
135. Lane M, Gardner DK (1992): Effect of incubation volume and embryo density on the development and viability of mouse embryos in vitro. Hum Reprod 7:558-562.
136. Lane M, Gardner DK (1994): Increase in postimplantation development of cultured mouse embryos by amino acids and induction of fetal retardation and exencephaly by ammonium ions. J Reprod Fertil 102:305-312.
137. Lane M, Gardner DK (1995): Removal of embryo-toxic ammonium from the culture medium by in situ enzymatic conversion to glutamate. J Exp Zool 271:356-363.
138. Lawitts JA, Biggers JD (1991): Overcoming the 2-cell block by modifying standard components in a mouse embryo culture medium. Biol Reprod 45:245-251.
139. Lawson RA, Rowson LE, Adams CE (1972a): The development of cow eggs in the rabbit oviduct and their viability after re-transfer to heifers. J Reprod Fertil 28:313-315.
140. Lawson RA, Adams CE, Rowson LE (1972b): The development of sheep eggs in the rabbit oviduct and their viability after re-transfer to ewes. J Reprod Fertil 29:105-116.
141. LeClerc C, Becker D, Buehr M, Warner A (1994): Low intracellular pH is involved in the early embryonic death of DDK mouse eggs fertilized by alien sperm. Dev Dynamics 200:257-267.
142. Leese HJ (1987): Analysis of embryos by non-invasive methods. Hum Reprod 2:37-40.
143. Leese HJ (1990): The energy metabolism of the preimplantation embryo. In S Heyner, LM Wiley (eds): "Early Embryo Development and Paracrine Relationships." New York: Alan R Liss Inc, pp 67-78.
144. Leese HJ (1991): Metabolism of the preimplantation mammalian embryo. Oxford Rev Reprod Biol 13:35-72.
145. Leese HJ (1995): Metabolic control during preimplantation mammalian development. Hum Reprod Update 1:63-72.
146. Leese HJ, Barton AM (1984): Pyruvate and glucose uptake by mouse ova and preimplantation embryos. J Reprod Fertil 72:9-13.
147. Leese HJ, Biggers JD, Mroz EA, Lechene C (1984): Nucleotides in a single mammalian ovum or preimplantation embryo. Anal Biochem 140:443-448.
148. Leese HJ, Hooper MA, Edwards RG, Ashwood-Smith MJ (1986): Uptake of pyruvate by early human embryos determined by a non-invasive technique. Hum Reprod 1:181-182.
149. Leese HJ, Conaghan J, Martin KL, Hardy K (1993): Early human embryo metabolism. Bioessays 15:259-264.
150. Lewis AMD, Kaye PL (1992): Characterization of glutamine uptake in mouse two-cell embryos and blastocysts. J Reprod Fertil 95:221-229.
151. Li J, Foote RH, Simkin M (1993): Development of rabbit zygotes cultured in protein-free medium with catalase, taurine, or superoxide dismutase. Biol Reprod 48:33-37.
152. Lindenau A, Fischer B (1994): Effect of oxygen concentration in the incubator's gas phase on the development of cultured preimplantation rabbit embryos. Theriogenology 41:889-898.
153. Magnusson C, Hillensjo T, Hamberger L. Nilsson L (1986): Oxygen consumption by human oocytes and blastocysts grown in vitro. Hum Reprod 1:183-184.
154. Martin KL, Leese HJ (1995): Role of glucose in mouse preimplantation embryo development. Mol Reprod Dev 40:436-443.
155. Martin KL, Hardy K, Winston RML, Leese HJ (1993): Activity of enzymes of energy metabolism in single human preimplantation embryos. J Reprod Fertil 99:259-266.
156. McGinnis LK, Youngs CR (1992): In vitro development of ovine embryos in CZB medium. Theriogenology 37:559-569.
157. McKiernan SH, Bavister BD (1990): Environmental variables influencing in vitro development of hamster 2-cell embryos to the blastocyst stage. Biol Reprod 43:404-413.
158. McKiernan SH, Bavister BD (1992): Different lots of bovine serum albumin inhibit or stimulate in vitro development of hamster embryos. In Vitro Cell Dev Biol 28A: 154-156.
159. MeKiernan SH, Bavister BD (1994): Timing of development is a critical parameter for predicting successful embryogenesis. Hum Reprod 9:2123-2129.
160. McKieman SH, Bavister BD, Tasca RJ (1991): Energy substrate requirements for in-vitro development of hamster 1- and 2-cell embryos to the blastocyst stage. Hum Reprod 6:64-75.
161. McKiernan SH, Clayton MK, Bavister BD (1995). Analysis of stimulatory and inhibitory amino acids for development of hamster one-cell embryos in vitro Mol Reprod Dev (in press).
162. McReynolds HD, Hadek R (1972): Periodic acid Schiff-positive material in hamster preimplantation embryos. J Reprod Fertil 30:173-175.
163. Mehta TS, Kiessling AA (1990): Development potential of mouse embryos conceived in vitro and cultured in ethylenediaminetetraacetic acid with or without amino acids or serum. Biol Reprod 43:600-606.
164. Menke TM, McLaren A (1970): Mouse blastoeysts grown in vivo and in vitro: carbon dioxide production and trophoblast outgrowth. J Reprod Fertil 23:117-127.
165. Meyen BA, Rosenkrans CF Jr, Davis DL (1989): Development of pig blastocysts in vitro is altered by serum, bovine serum albumin and amino acids and vitamins. Theriogenology 31:463-471.
166. Miller JGO, Schultz GA (1987): Amino acid content of preimplantation rabbit embryos and fluids of the reproductive tract. Biol Reprod 36:125-129.
167. Miller MH, Amos H, Sens DA (1981): Regulation of glucose utilization in chick embryo fibroblasts by bicarbonate ion. J Cell Physiol 107: 295-392.
168. Mills RM, Brinster RL (1967): Oxygen consumption of preimplantation mouse embryos. Exp Cell Res 47:337-344.
169. Miyoshi K, Funahashi H, Okuda K, Niwa K (1994): Development of rat one-cell embryos in a chemically defined medium: Effects of glucose, phosphate and osmolarity. J Reprod Fertil 100:21-26.
170. Moore K, Bondioli KR (1993): Glycine and alanine supplementation of culture medium enhances development of in vitro matured and fertilised cattle embryos. Biol Reprod 48:833-840.
171. Moore RW, Brinster RL (1973): Activity of mitochondrial enzymes in mouse embryos. J Reprod Fertil 35:37-43.
172. Muggleton-Harris AL, Brown JJG (1988): Cytoplasmic factors influence mitochondrial reorganization and resumption of cleavage during culture of early mouse embryos. Hum Reprod 3:1020-1028.
173. 14C-labelled pyruvate, glucose and carbon dioxide. J Reprod Fertil 24:287-290.
174. 3H)uridine into the major classes of RNA in mouse embryos during preimplantation development. Aust J Biol Sci 26:889-901.
175. Nagao Y, Saeki K, Hoshi M, Kainuma H (1994): Effects of oxygen concentration and oviductal epithelial tissue on the development of in vitro matured and fertilized bovine oocytes cultured in proteinfree medium. Theriogenology 41:681-687
176. Nakao H, Nakatsuji N (1990): Effects of co-culture, medium components and gas phase on in vitro culture of in vitro matured and in vitro fertilised bovine embryos. Theriogenology 33:591-600.
177. Nasr-Esfahani MH, Winston NJ, Johnson MH (1992): Effect of glucose, glutamine, ethylenediaminetetraacetic acid and oxygen tension on the concentration of reactive oxygen species and on development of the mouse preimplantation embryo in vitro. J Reprod Fertil 96:219-231.
178. Nichol R, Hunter RHF, Gardner DK, Leese HJ, Cooke GM (1992): Concentrations of energy substrates in oviductal fluid and blood plasma of pigs during the peri-ovulatory period. J Reprod Fertil 96:699-707.
179. Nicholls TJ, Leese HJ, Bronk JR (1983): Transport and metabolism of glucose by rat small intestine. Biochem J 212:183-187.
180. Noske IG, Daniel JC Jr (1974): Changes in uterine and oviductal fluid proteins during early pregnancy in the golden hamster. J Reprod Fertil 38:173-176.
181. Nota V, Campo R, Roziers P, Swinnen K, Vercruyssen M, Gordts S (1993): Mitochondrial distribution after fast embryo freezing. Hum Reprod 8:2115-2118.
182. O'Fallon JV, Wright RW Jr (1986): Quantitative determination of the pentose phosphate pathway in preimplantation mouse embryos. Biol Reprod 34:58-64.
183. 5-3H]glucose. Anal Biochem 162:33-38.
184. O'Fallon JV, Wright RW Jr (1991): Methyl viologen as a preferred electron acceptor in metabolic experiments. Anal Biochem 198:179-183.
185. O'Fallon JV, Wright RW JR (1995): Pyruvate revisited: A non-metabolic role for pyruvate in preimplantation embryo development. Theriogenology 43:288 (abs).
186. Olive PL (1989): Hydroethidine: A fluorescent redox probe for locating hypoxic cells in spheroids and murine tumours. Br J Cancer 60:332-338.
187. Orsini MW, McLaren A (1967): Loss of the zona pellucida in mice, and the effect of tubal ligation and ovariectomy. J Reprod Fertil 13:485-499.
188. Overstrom EW, Duby RT, Dobrinsky J, Roche JF, Boland MP (1992): Viability and oxidative metabolism of the bovine blastocyst. Theriogenology 37:269.
189. Ozias CB, Stern S (1973): Glycogen levels of preimplantation mouse embryos developing in vitro. Biol Reprod 8:467-472.
190. Pabon JE, Findley WE, Gibbons WE (1989): The toxic effect of short exposures to the atmospheric oxygen concentration on early mouse embryonic development. Fertil Steril 51:896-900.
191. Paetkau V, Lardy HA (1967): Phosphofructokinase. Correlation of physical and enzymatic properties. J Biol Chem 242:2035-2042.
192. Paria BC, Dey SK (1990): Preimplantation embryo development in vitro: Cooperative interactions among embryos and role of growth factors. Proc Natl Acad Sci USA 87:4756-4760.
193. Pemble LB, Kaye PL (1976): Whole protein uptake and metabolism by mouse blastocysts. J Reprod Fertil 78:149-157.
194. Perticarari S, Presani G, Mangiarotti MA, Banfi E (1991): Simultaneous flow cytometric method to measure phagocytosis and oxidative products by neutrophils. Cytometry 12:687-693.
195. Petters RM, Johnson BH, Reed ML, Archibong AE (1990): Glucose, glutamine and inorganic phosphate in early development of the pig embryo in vitro. J Reprod Fertil 89:269-275.
196. Peura TT (1990): Effects of acetate, citrate and malate on development of in vitro fertilized bovine embryos. Theriogenology 33:296.
197. Pike IL, Wales RG (1982): Uptake and incorporation of glucose especially into the glycogen pools of preimplantation mouse embryos during culture in vitro. Aust J Biol Sci 35:195-206.
198. Pike IL, Murdoch RN, Wales RG (1973): The use of in-vitro pulsechase culture techniques in studies of the incorporation of radioactive energy substrates by the preimplantation mouse embryo. J Reprod Fertil 32:314-315.
199. Pincus G (1941): The control of ovum growth. Science 93:438-439.
200. Pinyopummintr T, Bavister BD (1991): In vitro-matured/in vitro-
201. Pinyopummintr T, Bavister BD (1994): Development of bovine embryos in a cell-free culture medium: Effects of type of serum, timing of its inclusion and heat inactivation. Theriogenology 41:1241-1249.
202. Pinyopummintr T, Bavister BD (1995): Minimum energy substrate requirements for early cleavage stages of bovine embryo development in vitro. Theriogenology 43:299 (abs.).
203. Plante L, King WA (1994): Light and electron microscopic analysis of bovine embryos derived by in vitro and in vivo fertilization. J Assist Reprod Genet 11:515-529.
204. Powers RD, Tupper JT (1977): Developmental changes in membrane transport and permeability in the early mouse embryo. Dev Biol 56:306-315.
205. Prather RS, Sims MM, First NL (1991): Culture of porcine embryos from the one and two-cell stage to the blastocyst stage in sheep oviducts. Theriogenology 35:1147-1151.
206. Quinn P, Harlow GM (1978): The effect of oxygen on the development of preimplantation mouse embryos in vitro J Exp Zool 206:73-80.
207. Quinn P, Mompanah R, Steinberg JM, Weatherbee PS (1995): Successful human in vitro fertilization using a modified human tubal fluid medium lacking glucose and phosphate ions. Fertil Steri 63: 922-924.
208. Quinn P, Wales RG (1971): Adenosine triphosphate content of preimplantation mouse embryos. J Reprod Fertil 25:133-135.
209. Quinn P, Wales RG (1973a): The relationships between the ATP content of preimplantation mouse embryos and their development in vitro during culture. J Reprod Fertil 35:301-309.
210. Quinn P, Wales RG (1973b). Growth and metabolism of preimplantation mouse embryos cultured in phosphate-buffered medium. J Reprod Fertil 35:289-300
211. Quinn P, Whittingham DG (1982): Effects of fatty acids on fertilization and development of mouse embryos in vitro. J Androl 3:440-444.
212. Quinn P, Barros C, Whittingham DG (1982): Preservation of hamster oocytes to assay the fertilizing capacity of human spermatozoa. J Reprod Fertil 66:161-168.
213. Restall BJ, Wales RG (1966a): The fallopian tube of the sheep. 3. The chemical composition of the fluid from the fallopian tube. Aust J Biol Sci 19:687-698.
214. Restall BJ, Wales RG (1966b): The fallopian tube of the sheep. IV. The metabolism of ram spermatozoa in the presence of fluid from the fallopian tube. Aust J Biol Sci 19:883-893.
215. Restall BJ, Wales RG (1968): The fallopian tube of the sheep. V. Secretion from the ampulla and isthmus. Aust J Biol Sci 21:491-498.
216. Rieger D (1984): The measurement of metabolic activity as an approach to evaluating viability and diagnosing sex in early embryos. Theriogenology 21:138-149.
217. Rieger D, Guay P (1988): Measurement of the metabolism of energy substrates in individual bovine blastocysta. J Reprod Fertil 83:585-591.
218. Rieger D, Palmer E, Langeau D, Tiffin G (1989): Simultaneous measurement of the metabolism of two energy substrates in individual horse and cow embryos. Theriogenology 31:249 (abs).
219. Rieger D, Loskutoff NM, Betteridge KJ (1992a): Developmentally related changes in the metabolism of glucose and glutamine by cattle embryos produced and co-cultured in vitro. J Reprod Fertil 95:585-595.
220. Rieger D, Loskutoff NM, Betteridge KJ (1992b): Developmentally related changes in the uptake and metabolism of glucose, glutamine and pyruvate by cattle embryos produced in vitro. Reprod Fertil Dev 4:547-557.
221. Robinson DH, Benos DJ (1991): Glucose metabolism in the trophectoderm and inner cell mass of rabbit embryos. J Reprod Fertil 91:493-499.
222. Rodriguez GG, Phipps D, Ishiguro K, Ridgway HF (1992): Use of fluorescent redox probe for direct visualization of actively respiring bacteria. 58:1801-1808.
223. Rosenkrans CF Jr, First NL (1994): Effect of free amino acids and vitamins on cleavage and developmental rate of bovine zygotes in vitro. J Anim Sci 72:434-137.
224. Rosenkrans CF Jr, Zeng GQ, McNamara GT, Schoff PK, First NL (1993): Development of bovine embryos in vitro as affected by energy substrates. Biol Reprod 49:459-462.
225. Rozell MD, Williams JE, Butler JE (1992): Changes in concentration of ATP and ADP in individual preimplantation sheep embryos. Biol Reprod 47:866-870.
226. Schini SA, Bavister (1988): Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose. Biol Reprod 39:1183-1192.
227. Schulte RM (1993): Regulation of zygotic gene activation in the mouse. Bioessays 15:531-538.
228. Schultz GA, Heyner S (1993): Growth factors in preimplantation mammalian embryos. Oxf Rev Reprod Biol 15:43-82.
229. Sellens MH, Stein S, Sherman MI (1981): Protein and free amino acid content in preimplantation mouse embryos and in blastocysts under various culture conditions. J Reprod Fertil 61:307-315.
230. Seshagiri PB, Bavister BD (1989a): Glucose inhibits development of hamster S-cell embryos in vitro. Biol Reprod 40:599-606.
231. Seshagiri PB, Bavister BD (1989b): Phosphate is required for inhibition by glucose of development of hamster 8-cell embryos in vitro. Biol Reprod 40:607-514.
232. Seshagiri PB, Bavister BD (1991a): Glucose and phosphate inhibit respiration and oxidative metabolism in cultured hamster eight-cell embryos: Evidence for the 'Crabtree effect.' Mol Reprod Dev 30:105-111.
233. Seshagiri PB, Bavister BD (1991b): Relative development abilities of hamster 2- and 8-cell embryos cultured in hamster embryo culture medium-1 and -2. J Exp Zool 257:51-57.
234. Seshagiri PB, Bavister BD, Williamson JL, Aiken JM (1990): Qualitative comparison of protein production at different stages of hamster preimplantation embryo development. Cell Differentiation Dev 31: 161-168.
235. Sugawara S, Umezu M (1961): Studies on the metabolism of the mammalian ova II. Oxygen consumption of the cleaved ova of the rat. Tohoku J Agric Res 12:17-28.
236. Takahashi Y, First NL (1992): In vitro development of bovine one-cell embryos: Influence of glucose, lactate, pyruvate, amino acids and vitamins. Theriogenology 37:963-978.
237. 2+-dependent inner-membrane permeability transition. Biochem J 294:719-725.
238. Telford NA, Watson AJ, Schultz GA (1990): Transition from maternal to embryonic control in early mammalian development: A comparison of several species. Mol Reprod Dev 26:90-100.
239. Tervit HR, Whittingham DG, Rowson LEA (1972): Successful culture in vitro of sheep and cattle ova. J Reprod Fertil 30:493-497.
240. Thompson JGE, Parton GAJ, Cruickshank GW, Smith JF, Wales RG (1989): Development of sheep preimplantation embryos in media supplemented with glucose and acetate. Theriogenology 32:323-330.
241. Thompson JGE, Simpson AC, Pugh PA, Donnelly PE, Tervit HR (1990): Effect of oxygen concentration on in-vitro development of preimplantation sheep and cattle embryos. J Reprod Fertil 89:573-578.
242. Thompson JGE, Simpson AC, Pugh PA, Wright RWJ, Tervit HR (1991): Glucose utilisation by sheep embryos derived in vivo and in vitro. Reprod Fertil Dev 3:571-576.
243. Thompson JG, Simpson AC, Pugh PA, McGowan LT, James RW, Berg DK, Berg DK, Payne SR, Tervit HR (1992a): Effect of glucose level in culture medium on survival of in vitro cultured sheep embryos followed transfer to recipient ewes. Proc N Z Soc Anim Prod 52:255-256.
244. Thompson JG, Simpson AC, Pugh PA, Tervit HR (1992b): Requirement for glucose during in vitro culture of sheep preimplantation embryos. Mol Reprod Dev 31:253-257.
245. Thompson JG, Bell ACS, Pugh PA, Tervit HR (1993): Metabolism of pyruvate by pre-elongation sheep embryos and effect of pyruvate and lactate concentrations during culture in vitro. Reprod Fertil Dev 5:417-423.
246. Thompson JG, Partridge RJ, Houghton FD, Kennedy CJ, Pullar D, Wrathall AE, Leese HJ (1995): Preliminary observations on the uptake of oxygen by day 7 bovine blastocysts. Theriogenology 43: 337.
247. Thomson JL (1967): Effect of inhibitors of carbohydrate metabolism on the development of preimplantation mouse embryos. Exp Cell Res 41:411-427.
248. Tiffin GJ, Rieger D, Betteridge KJ, Yadav BR, King WA (1991): Glucose and glutamine metabolism in preattachment cattle embryos in relation to sex and stage of development. J Reprod Fertil 93:125-132.
249. Tokura T, Noda Y, Goto Y, Mori T (1993): Sequential observation of mitochondrial distribution in mouse oocytes and embryos. J Assisted Reprod Dev 10:417-426.
250. Tsai FCH, Gardner DK (1994): Nicotinamide, a component of complex culture media, inhibits mouse embryo development in vitro and reduces subsequent developmental potential after transfer. Fertil Steril 61:376-382.
251. Umaoka Y, Noda Y, Nakayama T. Narimoto K, Mori T, Iritani A (1993): Development of hamster one-cell embryos recovered under different conditions to the blastocyst stage. Theriogenology 39:485-498
252. Van Blerkom J, Runner MN (1984): Mitochondrial reorganization during resumption of arrested meiosis in the mouse oocyte. Am J Anat 171:335-355.
253. Van Blerkom J, Davis PW, Lee J (1995). ATP content of human oocytes and developmental potential and outcome after in vitro fertilization and embryo transfer. Hum Reprod 10:415-424.
254. Van Winkle LJ, Dickinson HR (1995): Differences in amino acid content of preimplantation mouse embryos that develop in vitro versus in vivo: In vitro effects of five amino acids that are abundant in oviductal secretions. Biol Reprod 52:96-104.
255. Voelkel SA, Hu YX (1992): Effect of gas atmosphere on the development of one-cell bovine embryos in two culture systems. Theriogenology 37:1117-1131.
256. Wales RG (1969): Accumulation of carboxylic acids from glucose by the pre-implantation mouse embryo. Aust J Biol Sci 22:701-707.
257. Wales RG (1973): The uterus of the ewe. II. Chemical analysis of uterine fluid collected by cannulation. Aust J Biol Sci 26:947-959.
258. Wales RG (1975): Maturation of the mammalian embryo: Biochemical aspects. Biol Reprod 12:66-81.
259. Wales RG (1986). Measurement of metabolic turnover in single mouse embryos. J Reprod Pertil 76:717-725.
260. Wales RG, Biggers JD (1968): The permeability of two- and eight-cell mouse embryos to L-malic acid. J Reprod Fertil 15:103-111.
261. Wales RG, Brinster RL (1968): The uptake of hexosesby pre-implantation mouse embryos in vitro. J Reprod Fertil 15:415-422.
262. Wales RG, Du ZF (1993): Contribution of the pentose phosphate pathway to glucose utilization by preimplantation sheep embryos. Reprod Fertil Dev 5:329-340.
263. Wales RG, Du ZF (1994): The metabolism of glutamine by the preimplantation sheep conceptus and its interaction with glucose. Reprod Fertil Dev 6:659-667.
264. Wales RG, Whittingham DG (1971): Decomposition of sodium pyruvate in culture media stored at 5 degrees C and its effects on the development of the preimplantation mouse embryo. J Reprod Fertil 24:126.
265. Wales RG, Whittingham DG, Hardy K, Craft IL (1987): Metabolism of glucose by human embryos. J Reprod Fertil 79:289-297.
266. Watson AJ, Watson PH, Warnes D, Walker SK, Armstrong DT, Seamark RF (1994): Preimplantation development of in vitro-matured and in vitro- fertilized ovine zygotes: Comparison between coculture on oviduct epithelial cell monolayers and culture under low oxygen atmosphere. Biol Reprod 50:715-724.
267. Waugh EE, Wales RG (1993): Oxidative utilization of glucose, acetate and lactate by early preimplantation sheep, mouse and cattle embryos. Reprod Fertil Dev 5:123-133.
268. Whitten WK (1956): Culture of tubal mouse ova. Nature 177:96.
269. Whitten WK (1957): Culture of tubal ova Nature 179:1081-1082.
270. Whitten WK (1971): Nutrient requirements for the culture of preimplantation embryos in vitro. In Raspe G (ed): "Advances in the Biosciences," Vol 6. New York: Pergamon Press, pp 129-141.
271. Whitten WK, Biggers JD (1968): Complete development in vitro of the pre-implantation stages of the mouse in a simple chemically defined medium. J Reprod Fertil 17:399-401.
272. Whittingham DG (1971): Culture of mouse ova. J Reprod Fertil (Suppl) 14:7-21.
273. Wiebold JL, Anderson GB (1985): Lethality of a tritiated amino acid in early mouse embryos. J Embryol Exp Morphol 88:209-217.
274. Wiley LM (1987): Development of the blastocyst: Role of cell polarity in cavitation and cell differentiation. In Bavister BD (ed): "The Mammalian Preimplantation Embryo: Regulation of Growth and Differentiation in Vitro." New York: Plenum Press, pp 65-93.
275. Winkler BS (1989): Retinal aerobic glycolysis revisited. Invest Ophthalmoi Vis Sci 30:1023.
276. Zarca A, Rubinstein S, Breitbart H (1988): Transport mechanism for calcium and phosphate in ram spermatozoa. Biochim Biophys Acta 944:351-358.
277. Zhang X, Armstrong DT (1990): Presence of amino acids and insulin in a chemically defined medium improves development of 8-cell rat embryos in vitro and subsequent implantation in vivo. Biol Reprod 42:662-668.