Direct cloning of PCR products amplified with Pwo DNA polymerase

BioTechniques

Volumn 20, Issue 2, 1996, Pages 186-188

  Hinnisdaels, Stefan ^a,b   Del Favero, Jurgen ^a   Vauterin, Marc ^a  

^a FREE UNIVERSITY OF BRUSSELS   (Belgium)

^b NFWO   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DNA POLYMERASE;

ARTICLE; GENE AMPLIFICATION; LABORATORY TEST; MOLECULAR CLONING; NONHUMAN; PLANT; POLYMERASE CHAIN REACTION;

EID: 0030065142     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96202bm05     Document Type: Article

References (6)

1. Costa, G.L. and M.P. Weiner. 1994. Polishing with T4 and Pfu polymerase increases the efficiency of cloning of PCR fragments. Nucleic Acids Res. 22:2423.
2. Costa, G.L. and M.P. Weiner. 1994. Protocols for cloning and analysis of blunt-ended PCR-generated DNA fragments. PCR Methods Appl. 3:S95-S106.
3. Hu, G. 1993. DNA polymerase-catalyzed addition of non-templated extra nucleotides to the 3′ end of a DNA fragment. DNA Cell Biol. 12:763-770.
4. Innis, A.M., D.H. Gelfand, J.J. Sninsky and T.J. White. 1990. PCR Protocols. A Guide to Methods and Applications. Academic Press, San Diego.
5. Mead, D.A., N.K. Pey, C. Hernnstadt, R.A. Marcil and L.M. Smith. 1991. A universal method for the direct cloning of PCR amplified nucleic acid. Bio/Technology 9:657-663.
6. Sambrook, J., E.F. Fritseh and T. Maniatis. 1989. Molecular Cloning- A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.