Mutational analysis of capping protein function in Saccharomyces cerevisiae

Molecular Biology of the Cell

Volumn 7, Issue 1, 1996, Pages 1-15

  Sizonenko, Gennady I ^a   Karpova, Tatiana S ^a   Gattermeir, David J ^a   Cooper, John A ^a,b  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Box 8228 ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL PROTEIN; MUTANT PROTEIN;

ACTIN FILAMENT; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; FUNGAL GENETICS; FUNGUS GROWTH; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

EID: 0030064644     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.7.1.1     Document Type: Article

References (34)

1. Adams, A.E., Cooper, J.A., and Drubin, D.G. (1993). Unexpected combinations of null mutations in genes encoding the actin cytoskeleton are lethal in yeast. Mol. Biol. Cell 4, 459-468.
2. Amatruda, J.F, Cannon, J.F., Tatchell, K., Hug, C., and Cooper, J.A. (1990). Disruption of the actin cytoskeleton in yeast capping protein mutants. Nature 344, 352-354.
3. Amatruda, J.F., and Cooper, J A. (1992). Purification, characterization and immunofluorescence localization of Saccharomyces cerevisiae capping protein. J. Cell Biol. 117, 1067-1076.
4. Amatruda, J.F., Gattermeir, D.J., Karpova, T.S., and Cooper, J A. (1992). Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution and polarized secretion in yeast J. Cell Biol. 119, 1151-1162.
5. Caldwell, J.E., Heiss, S.G., Mermall, V, and Cooper, J.A. (1989). Effects of CapZ, an actin capping protein of muscle, on the polymerization of actin. Biochemistry 28, 8506-8514.
6. 2-terminal domains of the α1 and β-subunits are not required for actin capping, and α1β and α2β heterodimers bind differentially to actin. J. Biol. Chem. 269, 6992-6998.
7. Christianson, T.W., Sikorski, R.S., Dante, M., Shero, J.H., and Hieter, P. (1992). Multifunctional yeast high-copy-number shuttle vectors. Gene 110, 119-122.
8. Cooper, J.A., Amatruda, J.F., Hug, C., and Schafer, D.A. (1993). Capping proteins. In: Guidebook to the Cytoskeletal and Motor Proteins, ed. T. Kreis and R. Vale, New York: Oxford University Press, 34-35.
9. Deng, W.P., and Nickoloff, J.A. (1992). Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 200, 81-88.
10. Eddy, R.J., Sauterer, R.A., and Condeelis, J.S. (1993). Aginactin, an agonist-regulated F-actin capping activity is associated with an Hsc70 in Dictyostelium. J. Biol. Chem. 268, 23267-23274.
11. Egelman, E.H., and Orlova, A. (1995). New insights into actin filament dynamics. Curr. Opin. Struct. Biol. 5, 172-180.
12. Friederich, E., Vancompernolle, K., Huet, C., Goethals, M., Finidori, J., Vandekerckhove, J., and Louvard, D. (1992). An actin-binding site containing a conserved motif of charged amino acid residues is essential for the morphogenic effect of villin. Cell 70, 81-92.
13. Haus, U., Trommler, P., Fisher, P.R., Hartmann, H., Lottspeich, F., Noegel, A.A., and Schleicher, M. (1993). The heat shock cognate protein from Dictyostelium affects actin polymerization through interaction with the actin-binding protein cap32/34 EMBO J. 12, 3763-3771.
14. Heiss, S.G., and Cooper, J.A. (1991). Regulation of CapZ, an actin capping protein of chicken muscle, by anionic phospholipids. Biochemistry 30, 8753-8758.
15. Hug, C., Jay, P.Y., Reddy, I, McNally, J.G., Bridgman, P.C., Elson, E.L., and Cooper, J.A. (1995). Capping protein levels influence actin assembly and cell motility in Dictyostelium. Cell 81, 591-600.
16. Hug, C., Miller, T.M., Torres, M.A., Casella, J F., and Cooper, J A. (1992). Identification and characterization of an actin-binding site of CapZ. J. Cell Biol. 116, 923-931.
17. Kaiser, C., Michaelis, S., and Mitchell, A. (1994). Methods in Yeast Genetics, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
18. Karpova, T.S., Lepetit, M.M., and Cooper, J.A. (1993). Mutations that enhance the cap2 null mutant phenotype in Saccharomyces cerevisiae affect the actin cytoskeleton, morphogenesis, and pattern of growth. Genetics 135, 693-709.
19. Karpova, T.S., Tatchell, K., and Cooper, J.A. (1995). Actin filaments in yeast are unstable in the absence of capping protein or fimbrin. J. Cell Biol. 131 (in press).
20. Kunkel, T.A., Bebenek, K., and McClary, J. (1991). Efficient site-directed mutagenesis using uracil-containing DNA. Methods Enzymol. 204, 125-139.
21. Moon, A.L., Janmey, P.A., Louie, K.A., and Drubin, D. (1993). Cofilin is an essential component of the yeast cortical cytoskeleton. J. Cell Biol. 120, 421-435.
22. Ohya, Y., and Botstein, D. (1994). Diverse essential functions revealed by complementing yeast calmodulin mutants. Science 263, 963-966.
23. Pollard, T.D., and Cooper, J.A. (1982). Methods to characterize actin filament networks. Methods Enzymol. 85, 211-233.
24. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
25. Sanger, F., Nicklen, S., and Coulson, A.R. (1977). DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
26. Schafer, D.A., Hug, C., and Cooper, J.A. (1995). Inhibition of CapZ during myofibrillogenesis alters assembly of actin filaments. J. Cell Biol. 128, 61-70.
27. Schafer, D.A., Korshunova, Y.O., Schroer, T.A., and Cooper, J.A. (1994). Differential localization and sequence analysis of capping protein β-subunit isoforms of vertebrates. J. Cell Biol. 127, 453-465.
28. Schafer, D.A., Mooseker, M.S., and Cooper, J.A. (1992). Localization of capping protein in chicken epithelial cells by immunofluorescence and biochemical fractionation. J. Cell Biol. 118, 335-346.
29. Schuler, G.D., Altschul, S.F., and Lipman, D.J. (1991). A workbench for multiple alignment construction and analysis. Proteins Struct Funct Genet 9, 180-190.
30. Sikorski, R.S., and Hieter, P. (1989). A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27.
31. Wada, K., Wada, Y., Ishibashi, F., Gojobori, T., and Ikemura, T. (1992). Codon usage tabulated from the GenBank genetic sequence data. Nucleic Acids Res. 20 (suppl), 2111-2118.
32. Waddle, J.A., Cooper, J.A., and Waterston, R.H. (1993). The α and β subunits of nematode actin capping protein function in yeast. Mol. Biol. Cell 4, 907-917.
33. Welch, M.D., Holtzman, D.A., and Drubin, D.G. (1994). The yeast actin cytoskeleton. Curr. Opin. Cell Biol. 6, 110-119.
34. Wertman, K.F., Drubin, D.G., and Botstein, D. (1992). Systematic mutational analysis of the yeast ACT1 gene. Genetics 132, 337-350.