Unusual effect of CO on C2H2 reduction by V nitrogenase from Azotobacter vinelandii

Journal of the American Chemical Society

Volumn 118, Issue 1, 1996, Pages 279-280

  Cameron, Linda M ^a   Hales, Brian J ^a  

^a LOUISIANA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0030063724     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja953124y     Document Type: Article

References (22)

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18. 20 In these experiments, dithionite (20 mM) was used as a reductant. For the data indicated in the figure, the partial pressure of acetylene was 0.1 atm. Data collected at different partial pressures ranging from 0.1 to 0.002 atm showed no substrate dependencies, as expected for noncompetitive interaction The indicated partial pressure of CO was produced using a Pressure-Lok syringe (Precision Sampling, Baton Rouge, LA) to replace a volume of headspace gas over the reaction mixture with an equal volume of 100% CO (Scott Specialty Gases, Houston, TX) from a CO tank fitted with an Oxysorb cartridge (Messer Griesheim, Valley Forge. PA). Fits to eq 6 with the data were obtained with FFIT 2,2-S, a function-fitting program (A Soft Answer. Macquarie Centre. NSW, Australia).
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