Quantitation of the nucleophosmin/B23-translocation using imaging analysis

Cancer Letters

Volumn 100, Issue 1-2, 1996, Pages 191-197

  Chan, Pui K ^a   Qi, Yi ^a   Amley, Josie ^a   Koller, Charles A ^b  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

Actinomycin D; Anthracyclines; Immunofluorescence; Nucleophosmin; Nucleophosmin B23 translocation

Indexed keywords

ACLACINOMYCIN; ANTHRACYCLINE; DACTINOMYCIN; DAUNORUBICIN; NUCLEOPHOSMIN;

ARTICLE; BREAST CARCINOMA; CELL NUCLEUS; CELL STRAIN MCF 7; CONTROLLED STUDY; DOSE TIME EFFECT RELATION; HUMAN; HUMAN CELL; IMAGE ANALYSIS; LEUKEMIA CELL; NUCLEOLUS; PRIORITY JOURNAL; PROTEIN TRANSPORT; QUANTITATIVE ASSAY;

EID: 0030063697     PISSN: 03043835     EISSN: None     Source Type: Journal    
DOI: 10.1016/0304-3835(95)04100-1     Document Type: Article

References (14)

1. Chan, W.Y., Liu, Q.R., Borjigin, J., Busch, H., Rennert, O.R., Tease, L.A. and Chan, P.K. (1989) Characterization of the cDNA encoding human nucleophosmin and studies of its role in normal and abnormal growth. Biochemistry, 28, 1033-1039.
2. Yung, B.Y.M., Busch, R.K., Busch, H., Mauger, A.B. and Chan, P.K. (1985) Effects of actinomycin D analogs on nucleolar phosphoprotein B23 (37 kD/pI 5.1). Biochem. Pharm., 34, 4059-4063.
3. Yung, B.Y.M., Bor, M.S. and Chan, P.K. (1990) Short exposure of actinomycin D induced 'reversible' translocation of protein B23, inhibition of cell growth and RNA synthesis. Cancer Res., 50, 5987-5991.
4. Finch, R. A. and Chan, P.K. (1992) Studies of actinomycin D induced B23-translocation in P388D1 cells implanted in DBA/2 mice. Oncology, 49, 223-226.
5. Yung, B.Y.M., Busch, H. and Chan, P.K. (1986) Effects of luzo-peptins on protein B23 translocation and rRNA synthesis. Cancer Res., 46, 922-925.
6. Chan, P.K., Aldrich, M.B. and Yung, B.Y.M. (1987) Studies of nucleolar protein B23 translocation after doxorubicin treatment in murine tumor cells. Cancer Res., 47, 3798-3801.
7. Sweet, P., Chan, P.K. and Slater, L.M. (1989) Cyclosporin A and verapamil enhancement of daunomycin produced nucleolar protein B 23 translocation in daunomycin resistant and sensitive tumor cells. Cancer Res., 49, 677-680.
8. Chan, P.K. (1992) Characterization and cellular localization of nucleophosmin/B23 in HeLa cells treated with selected cytotoxic agents (studies of B23-translocation mechanism). Exp. Cell Res., 203, 174-181.
9. Yung, B.Y.M., Busch, H. and Chan, P.K. (1985) Translocation of nucleolar phosphoprotein B23 (37 kDa/pI 5.1) induced by selective inhibitors of ribosome synthesis. Biochim. Biophys. Acta, 826, 167-173.
10. Finch, R.A., Revankar, G.R. and Chan, P.K. (1993) Nucleolar localization of nucleophosmin/B23 requires GTP. J. Biol. Chem., 268, 5823-5827.
11. Chan, P.K., Aldrich, M.B. and Chakrabarty, S. (1988) Assessment of tumor cell sensitivity to mitomycin C by 'B23 translocation' assay. Cancer Lett., 40, 143-149.
12. Giloh, H. and Dedat, J.W. (1982) Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate. Science, 217, 1252-1255.
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14. Chan, P.K., Aldrich, M. and Busch, H. (1985) Alterations in immunolocalization of the phosphoprotein B23 in HeLa cells during serum starvation. Exp. Cell Res., 161, 101-110.